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Chemically induced polyploidization in Spathiphyllum wallisii Regel through somatic embryogenesis

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Abstract
Polyploid Spathiphyllum wallisii Regel 'Speedy' plants were obtained from somatic embryos induced on anther filaments exposed to mitosis inhibitors. Primary embryos yielded less polyploid plantlets than secondary embryos. Colchicine could be efficiently replaced by oryzalin or trifluralin (10 muM), resulting in an average yield of 5% polyploids. The mitosis inhibitors were directly added to the induction medium. Morphological differences between diploid and tetraploid S. wallisii hybrids were observed. Throughout our experiments flow cytometry was applied as an unambiguous screening tool. Ploidy breeding within the genus Spathiphyllum holds promises towards the development of tetraploid hybrids with an altered morphology, or triploids with a reduced fertility.
Keywords
in vitro, Araceae, tetraploid, oryzalin, colchicine, IN-VITRO, FLOW CYTOMETRIC ANALYSIS, NUCLEAR-DNA CONTENT, somatic embryo, trifluralin, INDUCTION, INVITRO, CULTURE, TETRAPLOIDY, COLCHICINE, HYBRIDS, ANGIOSPERMS

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Chicago
Eeckhaut, Tom, Stefaan Werbrouck, Leen WH Leus, Erik Van Bockstaele, and Pierre Debergh. 2004. “Chemically Induced Polyploidization in Spathiphyllum Wallisii Regel Through Somatic Embryogenesis.” Plant Cell Tissue and Organ Culture 78 (3): 241–246.
APA
Eeckhaut, T., Werbrouck, S., Leus, L. W., Van Bockstaele, E., & Debergh, P. (2004). Chemically induced polyploidization in Spathiphyllum wallisii Regel through somatic embryogenesis. PLANT CELL TISSUE AND ORGAN CULTURE, 78(3), 241–246.
Vancouver
1.
Eeckhaut T, Werbrouck S, Leus LW, Van Bockstaele E, Debergh P. Chemically induced polyploidization in Spathiphyllum wallisii Regel through somatic embryogenesis. PLANT CELL TISSUE AND ORGAN CULTURE. 2004;78(3):241–6.
MLA
Eeckhaut, Tom, Stefaan Werbrouck, Leen WH Leus, et al. “Chemically Induced Polyploidization in Spathiphyllum Wallisii Regel Through Somatic Embryogenesis.” PLANT CELL TISSUE AND ORGAN CULTURE 78.3 (2004): 241–246. Print.
@article{285698,
  abstract     = {Polyploid Spathiphyllum wallisii Regel 'Speedy' plants were obtained from somatic embryos induced on anther filaments exposed to mitosis inhibitors. Primary embryos yielded less polyploid plantlets than secondary embryos. Colchicine could be efficiently replaced by oryzalin or trifluralin (10 muM), resulting in an average yield of 5\% polyploids. The mitosis inhibitors were directly added to the induction medium. Morphological differences between diploid and tetraploid S. wallisii hybrids were observed. Throughout our experiments flow cytometry was applied as an unambiguous screening tool. Ploidy breeding within the genus Spathiphyllum holds promises towards the development of tetraploid hybrids with an altered morphology, or triploids with a reduced fertility.},
  author       = {Eeckhaut, Tom and Werbrouck, Stefaan and Leus, Leen WH and Van Bockstaele, Erik and Debergh, Pierre},
  issn         = {0167-6857},
  journal      = {PLANT CELL TISSUE AND ORGAN CULTURE},
  keyword      = {in vitro,Araceae,tetraploid,oryzalin,colchicine,IN-VITRO,FLOW CYTOMETRIC ANALYSIS,NUCLEAR-DNA CONTENT,somatic embryo,trifluralin,INDUCTION,INVITRO,CULTURE,TETRAPLOIDY,COLCHICINE,HYBRIDS,ANGIOSPERMS},
  language     = {eng},
  number       = {3},
  pages        = {241--246},
  title        = {Chemically induced polyploidization in Spathiphyllum wallisii Regel through somatic embryogenesis},
  url          = {http://dx.doi.org/10.1023/B:TICU.0000025659.19232.04},
  volume       = {78},
  year         = {2004},
}

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