Anchored PCR method as alternative to detect and identify GMO's
- Author
- Ilse Theuns, Sylvie De Buck (UGent) , Anna Depicker (UGent) , Dirk Van Gaver, André Huyghebaert (UGent) , Erik Van Bockstaele (UGent) and Marc De Loose (UGent)
- Organization
- Abstract
- More and more genetically engineeredproducts are being developed and are coming to the market. Within the EU labelling ofgenetically modifiedfoods is mandatary. This demands a control mechanism to checkpositive and negative labels. The DMIF-GENproject (Development ofMethods to Identify Foods produced by Genetic ENgineering) will develop 6 methodsfor identification of GMO’s and validate these methods by intercomparison testing. Encoded samples will be distributed and analysed by the partners. Besides this, R&D on simplified detection assays and alternative techniques like direct hybridisation will be performed. Since these methods are based on generation ofPCR amplifiedfragments, specific primers and probes are selected and applied. A disadvantage ofthe methods currently applied is that they do not offer the possibility to discriminate between different genetic Lines since they detect mostly conserved genetic dements in the transgenes such as promoters, terminators and antibiotic resistance genes. Line specific DNA fragments can onty be amplified when Information is supplied by the biotechnology companies. However, informalion is often confidential. Moreover, for control organisations it is important that the identification experiments can be carried out independentlyfrom the companies. Therefore, as an alternativeforthe specific PCR detection system, we propose to evaluate an anchored PCR-AFLP technique. This technique should allow to generale GMO specificfragments on the basis ofgeneral available informalion on the transgene present
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-278846
- MLA
- Theuns, Ilse, et al. “Anchored PCR Method as Alternative to Detect and Identify GMO’s.” MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT, no. 63/64b, 1998, pp. 1539–42.
- APA
- Theuns, I., De Buck, S., Depicker, A., Van Gaver, D., Huyghebaert, A., Van Bockstaele, E., & De Loose, M. (1998). Anchored PCR method as alternative to detect and identify GMO’s. MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT, (63/64b), 1539–1542.
- Chicago author-date
- Theuns, Ilse, Sylvie De Buck, Anna Depicker, Dirk Van Gaver, André Huyghebaert, Erik Van Bockstaele, and Marc De Loose. 1998. “Anchored PCR Method as Alternative to Detect and Identify GMO’s.” MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT, no. 63/64b: 1539–42.
- Chicago author-date (all authors)
- Theuns, Ilse, Sylvie De Buck, Anna Depicker, Dirk Van Gaver, André Huyghebaert, Erik Van Bockstaele, and Marc De Loose. 1998. “Anchored PCR Method as Alternative to Detect and Identify GMO’s.” MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT (63/64b): 1539–1542.
- Vancouver
- 1.Theuns I, De Buck S, Depicker A, Van Gaver D, Huyghebaert A, Van Bockstaele E, et al. Anchored PCR method as alternative to detect and identify GMO’s. MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT. 1998;(63/64b):1539–42.
- IEEE
- [1]I. Theuns et al., “Anchored PCR method as alternative to detect and identify GMO’s,” MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT, no. 63/64b, pp. 1539–1542, 1998.
@article{278846,
abstract = {{More and more genetically engineeredproducts are being developed and are coming to the
market. Within the EU labelling ofgenetically modifiedfoods is mandatary. This demands a
control mechanism to checkpositive and negative labels. The DMIF-GENproject (Development
ofMethods to Identify Foods produced by Genetic ENgineering) will develop 6 methodsfor
identification of GMO’s and validate these methods by intercomparison testing. Encoded samples
will be distributed and analysed by the partners. Besides this, R&D on simplified detection assays
and alternative techniques like direct hybridisation will be performed. Since these methods are
based on generation ofPCR amplifiedfragments, specific primers and probes are selected and
applied. A disadvantage ofthe methods currently applied is that they do not offer the possibility to
discriminate between different genetic Lines since they detect mostly conserved genetic dements in
the transgenes such as promoters, terminators and antibiotic resistance genes. Line specific DNA
fragments can onty be amplified when Information is supplied by the biotechnology companies.
However, informalion is often confidential. Moreover, for control organisations it is important
that the identification experiments can be carried out independentlyfrom the companies.
Therefore, as an alternativeforthe specific PCR detection system, we propose to evaluate an
anchored PCR-AFLP technique. This technique should allow to generale GMO specificfragments
on the basis ofgeneral available informalion on the transgene present}},
author = {{Theuns, Ilse and De Buck, Sylvie and Depicker, Anna and Van Gaver, Dirk and Huyghebaert, André and Van Bockstaele, Erik and De Loose, Marc}},
issn = {{0368-9697}},
journal = {{MEDEDELINGEN VAN DE FACULTEIT LANDBOUWWETENSCHAPPEN - RIJKSUNIVERSITEIT GENT}},
language = {{dut}},
number = {{63/64b}},
pages = {{1539--1542}},
title = {{Anchored PCR method as alternative to detect and identify GMO's}},
year = {{1998}},
}