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Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia franciscana

(1994) ANALYTICAL CHEMISTRY. 66(8). p.1330-1333
Author
Organization
Abstract
Two procedures are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia, The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1 M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254 nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7 +/- 3.5% versus 63.0 +/- 12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity.
Keywords
PERFORMANCE LIQUID-CHROMATOGRAPHY, DEHYDROASCORBIC ACID, ADENINE-NUCLEOTIDES, RAINBOW-TROUT, SULFATE, TISSUE, METABOLITE, RELEASE, HPLC

Citation

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Chicago
Nelis, HJ, G Merchie, Patrick Lavens, Patrick Sorgeloos, and Andreas De Leenheer. 1994. “Solid-phase Extraction of Ascorbic Acid 2-sulfate from Cysts of the Brine Shrimp Artemia Franciscana.” Analytical Chemistry 66 (8): 1330–1333.
APA
Nelis, HJ, Merchie, G., Lavens, P., Sorgeloos, P., & De Leenheer, A. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia franciscana. ANALYTICAL CHEMISTRY, 66(8), 1330–1333.
Vancouver
1.
Nelis H, Merchie G, Lavens P, Sorgeloos P, De Leenheer A. Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia franciscana. ANALYTICAL CHEMISTRY. 1994;66(8):1330–3.
MLA
Nelis, HJ, G Merchie, Patrick Lavens, et al. “Solid-phase Extraction of Ascorbic Acid 2-sulfate from Cysts of the Brine Shrimp Artemia Franciscana.” ANALYTICAL CHEMISTRY 66.8 (1994): 1330–1333. Print.
@article{257122,
  abstract     = {Two procedures are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia, The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1 M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254 nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7 +/- 3.5\% versus 63.0 +/- 12.7\%), reproducibility (CVs of 3.6-7.7\% versus 6.3-18.7\%), and simplicity.},
  author       = {Nelis, HJ and Merchie, G and Lavens, Patrick and Sorgeloos, Patrick and De Leenheer, Andreas},
  issn         = {0003-2700},
  journal      = {ANALYTICAL CHEMISTRY},
  keyword      = {PERFORMANCE LIQUID-CHROMATOGRAPHY,DEHYDROASCORBIC ACID,ADENINE-NUCLEOTIDES,RAINBOW-TROUT,SULFATE,TISSUE,METABOLITE,RELEASE,HPLC},
  language     = {eng},
  number       = {8},
  pages        = {1330--1333},
  title        = {Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia franciscana},
  url          = {http://dx.doi.org/10.1021/ac00080a019},
  volume       = {66},
  year         = {1994},
}

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