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Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma

(1992) JOURNAL OF EXPERIMENTAL MEDICINE. 176(4). p.1015-1024
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Abstract
We investigated the biological role of the human tumor necrosis factor p75 (hTNF-R75), making use of the species specificity of TNF responses in murine (m) T cell lines. Several TNF-mediated activities on mouse T cells, such as cytokine induction or proliferation, showed a 100-500-fold difference in specific biological activity between mTNF and hTNF. After transfection of hTNF-R75 cDNA in a rat/mouse T cell hybridoma (PC60), however, the 100-fold lower specific biological activity of hTNF was converted to the same specific biological activity as mTNF. The TNF-mediated induction of granulocyte./macrophage colony-stimulating factor was strongly synergized by the addition of interleukin 1. ln the presence of the latter cytokine, ligand-competing monoclonal antibodies against hTNF-R75 (utr-1, utr-2, utr-3) were agonistic on transfected PC60 cells. This agonistic activity was further enhanced by crosslinking with sheep anti-murine immunoglobulin antibodies. These data provide direct evidence for a functional role of TNF-R75, without ligand-dependent TNF-R55 involvement, in the induction of cytokine secretion in T cells.
Keywords
EXPRESSION, MOLECULAR-CLONING, PROLIFERATION, INTERLEUKIN-1, GROWTH-FACTOR, MONOCLONAL-ANTIBODIES, FACTOR TNF RECEPTOR, CYTOLYTIC ACTIVITY, FACTOR-ALPHA-CACHECTIN, GM-CSF

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Citation

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Chicago
Vandenabeele, Peter, Wim Declercq, Dominique Vercammen, Marc Van De Craen, Johan Grooten, Hansruedi Loetscher, Manfred Brockhaus, Werner Lesslauer, and Walter Fiers. 1992. “Functional Characterization of the Human Tumor Necrosis Factor Receptor P75 in a Transfected Rat/mouse T Cell Hybridoma.” Journal of Experimental Medicine 176 (4): 1015–1024.
APA
Vandenabeele, P., Declercq, W., Vercammen, D., Van De Craen, M., Grooten, J., Loetscher, H., Brockhaus, M., et al. (1992). Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma. JOURNAL OF EXPERIMENTAL MEDICINE, 176(4), 1015–1024.
Vancouver
1.
Vandenabeele P, Declercq W, Vercammen D, Van De Craen M, Grooten J, Loetscher H, et al. Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma. JOURNAL OF EXPERIMENTAL MEDICINE. 1992;176(4):1015–24.
MLA
Vandenabeele, Peter, Wim Declercq, Dominique Vercammen, et al. “Functional Characterization of the Human Tumor Necrosis Factor Receptor P75 in a Transfected Rat/mouse T Cell Hybridoma.” JOURNAL OF EXPERIMENTAL MEDICINE 176.4 (1992): 1015–1024. Print.
@article{232091,
  abstract     = {We investigated the biological role of the human tumor necrosis factor p75 (hTNF-R75), making use of the species specificity of TNF responses in murine (m) T cell lines. Several TNF-mediated activities on mouse T cells, such as cytokine induction or proliferation, showed a 100-500-fold difference in specific biological activity between mTNF and hTNF. After transfection of hTNF-R75 cDNA in a rat/mouse T cell hybridoma (PC60), however, the 100-fold lower specific biological activity of hTNF was converted to the same specific biological activity as mTNF. The TNF-mediated induction of granulocyte./macrophage colony-stimulating factor was strongly synergized by the addition of interleukin 1. ln the presence of the latter cytokine, ligand-competing monoclonal antibodies against hTNF-R75 (utr-1, utr-2, utr-3) were agonistic on transfected PC60 cells. This agonistic activity was further enhanced by crosslinking with sheep anti-murine immunoglobulin antibodies. These data provide direct evidence for a functional role of TNF-R75, without ligand-dependent TNF-R55 involvement, in the induction of cytokine secretion in T cells.},
  author       = {Vandenabeele, Peter and Declercq, Wim and Vercammen, Dominique and Van De Craen, Marc and Grooten, Johan and Loetscher, Hansruedi and Brockhaus, Manfred and Lesslauer, Werner and Fiers, Walter},
  issn         = {0022-1007},
  journal      = {JOURNAL OF EXPERIMENTAL MEDICINE},
  language     = {eng},
  number       = {4},
  pages        = {1015--1024},
  title        = {Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma},
  url          = {http://dx.doi.org/10.1084/jem.176.4.1015},
  volume       = {176},
  year         = {1992},
}

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