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Different types of peroxisomes in human duodenal epithelium

Frank Roels UGent, Marc Espeel UGent, M Pauwels, Dirk De Craemer UGent, H Egberts and P Van der Spek (1991) GUT. 32(8). p.858-865
abstract
Peroxisomes are ubiquitous organelles containing enzyme sequences for beta-oxidation of fatty acids, synthesis of bile acids, and ether phospholipids. In the inherited peroxisomal diseases one or more enzymes are deficient in hepatic, renal, and fibroblast peroxisomes. We have examined peroxisomes by light and electron microscopy in 29 duodenal biopsy specimens (21 with normal mucosa) after staining for catalase activity, a marker enzyme. Peroxisomes were most numerous in the apices of the nucleus and at the villus base. Two types were distinguished: rounded to oval forms with a median lesser diameter of 0.23-0.31-mu-m, and tubular, vermiform organelles 0.1-mu-m thick and up to 3-mu-m long. Both types coexist in most patients. Tilting of sections and examination of semithin sections at 120 kV did not show connections between individual organelles. By morphometry, volume density was at least 0.45-0.62% of cellular volume, compared to 1.05% in human liver. In contrast, in four out of five individuals surface density of the peroxisomal membrane was 1.4-2.3 times higher than in control livers; this is expected to favour the exchange of metabolites. We suggest that intestinal peroxisomes contribute substantially to the breakdown of very long chain fatty acids.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MUCOSA, MOUSE, ENZYMES, FATTY-ACIDS, NEONATAL-ONSET, ABSORPTIVE CELLS, ZELLWEGER SYNDROME, HEPATIC PEROXISOMES, BETA-OXIDATION, SMALL-INTESTINE
journal title
GUT
Gut
volume
32
issue
8
pages
858 - 865
Web of Science type
Article
ISSN
0017-5749
DOI
10.1136/gut.32.8.858
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
224479
handle
http://hdl.handle.net/1854/LU-224479
date created
2004-05-24 13:24:00
date last changed
2011-08-25 10:20:06
@article{224479,
  abstract     = {Peroxisomes are ubiquitous organelles containing enzyme sequences for beta-oxidation of fatty acids, synthesis of bile acids, and ether phospholipids. In the inherited peroxisomal diseases one or more enzymes are deficient in hepatic, renal, and fibroblast peroxisomes. We have examined peroxisomes by light and electron microscopy in 29 duodenal biopsy specimens (21 with normal mucosa) after staining for catalase activity, a marker enzyme. Peroxisomes were most numerous in the apices of the nucleus and at the villus base. Two types were distinguished: rounded to oval forms with a median lesser diameter of 0.23-0.31-mu-m, and tubular, vermiform organelles 0.1-mu-m thick and up to 3-mu-m long. Both types coexist in most patients. Tilting of sections and examination of semithin sections at 120 kV did not show connections between individual organelles. By morphometry, volume density was at least 0.45-0.62\% of cellular volume, compared to 1.05\% in human liver. In contrast, in four out of five individuals surface density of the peroxisomal membrane was 1.4-2.3 times higher than in control livers; this is expected to favour the exchange of metabolites. We suggest that intestinal peroxisomes contribute substantially to the breakdown of very long chain fatty acids.},
  author       = {Roels, Frank and Espeel, Marc and Pauwels, M and De Craemer, Dirk and Egberts, H and Van der Spek, P},
  issn         = {0017-5749},
  journal      = {GUT},
  keyword      = {MUCOSA,MOUSE,ENZYMES,FATTY-ACIDS,NEONATAL-ONSET,ABSORPTIVE CELLS,ZELLWEGER SYNDROME,HEPATIC PEROXISOMES,BETA-OXIDATION,SMALL-INTESTINE},
  language     = {eng},
  number       = {8},
  pages        = {858--865},
  title        = {Different types of peroxisomes in human duodenal epithelium},
  url          = {http://dx.doi.org/10.1136/gut.32.8.858},
  volume       = {32},
  year         = {1991},
}

Chicago
Roels, Frank, Marc Espeel, M Pauwels, Dirk De Craemer, H Egberts, and P Van der Spek. 1991. “Different Types of Peroxisomes in Human Duodenal Epithelium.” GUT 32 (8): 858–865.
APA
Roels, F., Espeel, M., Pauwels, M., De Craemer, D., Egberts, H., & Van der Spek, P. (1991). Different types of peroxisomes in human duodenal epithelium. GUT, 32(8), 858–865.
Vancouver
1.
Roels F, Espeel M, Pauwels M, De Craemer D, Egberts H, Van der Spek P. Different types of peroxisomes in human duodenal epithelium. GUT. 1991;32(8):858–65.
MLA
Roels, Frank, Marc Espeel, M Pauwels, et al. “Different Types of Peroxisomes in Human Duodenal Epithelium.” GUT 32.8 (1991): 858–865. Print.