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Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid

Author
Organization
Abstract
Objective: Our objective was to develop a new reliable method for cytogenetic analysis of the chromosome set in second polar bodies (PBs) from one-cell-stage mouse embryos. Setting: The study took place at the Reproductive Biology and Experimental Cytogenetics Laboratories. Methods: Oocytes from F1 hybrid and T6/T6 mice were fertilized in vitro and artificially activated with ethanol. Zygotes, parthenogenetic embryos, and isolated second PBs were treated with 10 muM okadaic acid (OA) for 1-2 hr, further cultured in plain medium, and fixed. Chromosomal preparations were made and C-banded, and the number of chromosomes in second PBs and embryos was counted. Results: OA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei. Countable chromosome plates were obtained in 92-93% of second PBs treated 4-4.5 hr after activation. The T6 marker chromosome could easily be recognized in second PBs from T6/T6 mice. A haploid set of chromosomes was obtained in 18 of 19 isolated second PBs treated with OA 4-5 hr after activation. Conclusion: Treatment of second PBs with OA allows visualization of the PB chromosomes. Cytogenetic analysis of the second PB and the corresponding oocyte constitutes a new approach for the study of meiotic nondisjunction in experimental cytogenetics. The chromosomal study of isolated second PBs seems to be promising for clinical preimplantation cytogenetics.
Keywords
MOUSE, SECOND POLAR BODY, OKADAIC ACID, CYTOGENETICS, MEIOTIC NONDISJUNCTION

Citation

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MLA
Dyban, AP, Petra De Sutter, D Dozortsev, et al. “Visualization of Second Polar Body Chromosomes in Fertilized and Artificially Activated Mouse Oocytes Treated with Okadaic Acid.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 9.6 (1992): 572–579. Print.
APA
Dyban, A., De Sutter, P., Dozortsev, D., & Verlinsky, Y. (1992). Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 9(6), 572–579.
Chicago author-date
Dyban, AP, Petra De Sutter, D Dozortsev, and Y Verlinsky. 1992. “Visualization of Second Polar Body Chromosomes in Fertilized and Artificially Activated Mouse Oocytes Treated with Okadaic Acid.” Journal of Assisted Reproduction and Genetics 9 (6): 572–579.
Chicago author-date (all authors)
Dyban, AP, Petra De Sutter, D Dozortsev, and Y Verlinsky. 1992. “Visualization of Second Polar Body Chromosomes in Fertilized and Artificially Activated Mouse Oocytes Treated with Okadaic Acid.” Journal of Assisted Reproduction and Genetics 9 (6): 572–579.
Vancouver
1.
Dyban A, De Sutter P, Dozortsev D, Verlinsky Y. Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 1992;9(6):572–9.
IEEE
[1]
A. Dyban, P. De Sutter, D. Dozortsev, and Y. Verlinsky, “Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid,” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, vol. 9, no. 6, pp. 572–579, 1992.
@article{224090,
  abstract     = {Objective: Our objective was to develop a new reliable method for cytogenetic analysis of the chromosome set in second polar bodies (PBs) from one-cell-stage mouse embryos.
Setting: The study took place at the Reproductive Biology and Experimental Cytogenetics Laboratories.
Methods: Oocytes from F1 hybrid and T6/T6 mice were fertilized in vitro and artificially activated with ethanol. Zygotes, parthenogenetic embryos, and isolated second PBs were treated with 10 muM okadaic acid (OA) for 1-2 hr, further cultured in plain medium, and fixed. Chromosomal preparations were made and C-banded, and the number of chromosomes in second PBs and embryos was counted.
Results: OA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei. Countable chromosome plates were obtained in 92-93% of second PBs treated 4-4.5 hr after activation. The T6 marker chromosome could easily be recognized in second PBs from T6/T6 mice. A haploid set of chromosomes was obtained in 18 of 19 isolated second PBs treated with OA 4-5 hr after activation.
Conclusion: Treatment of second PBs with OA allows visualization of the PB chromosomes. Cytogenetic analysis of the second PB and the corresponding oocyte constitutes a new approach for the study of meiotic nondisjunction in experimental cytogenetics. The chromosomal study of isolated second PBs seems to be promising for clinical preimplantation cytogenetics.},
  author       = {Dyban, AP and De Sutter, Petra and Dozortsev, D and Verlinsky, Y},
  issn         = {1058-0468},
  journal      = {JOURNAL OF ASSISTED REPRODUCTION AND GENETICS},
  keywords     = {MOUSE,SECOND POLAR BODY,OKADAIC ACID,CYTOGENETICS,MEIOTIC NONDISJUNCTION},
  language     = {eng},
  number       = {6},
  pages        = {572--579},
  title        = {Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid},
  url          = {http://dx.doi.org/10.1007/BF01204256},
  volume       = {9},
  year         = {1992},
}

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