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Differentiation of campylobacters and Campylobacter-like organisms by numerical analysis of one-dimensional electrophoretic protein patterns

Author
Organization
Abstract
The suitability of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and computer-assisted comparison of the protein profiles for differentiation, identification and typing of campylobacters was evaluated. One hundred and fifty reference strains representing all Campylobacter, Helicobacter and "Flexi-spira" species were included in a single electrophoretic study and the influence of growth medium, growth conditions and laboratory-specific manipulations was investigated for several strains. The results indicate that this technique is very useful in the study of campylobacters, as (i) all species are clearly differentiated in one or more electrophoretic clusters or subclusters, (ii) the identification does not depend on the factors cited above and (iii) within several species, different electrophoretic types can be distinguished.
Keywords
CAMPYLOBACTER-LIKE ORGANISMS, CAMPYLOBACTER, TAXONOMY, EPIDEMIOLOGY, ELECTROPHORESIS OF PROTEINS, NUMERICAL ANALYSIS, POLYACRYLAMIDE-GEL ELECTROPHORESIS, CATALASE-NEGATIVE CAMPYLOBACTERS, FATTY-ACID COMPOSITION, THERMOPHILIC CAMPYLOBACTERS, BACTEROIDES-GRACILIS, DNA HOMOLOGY, SP-NOV, BACTERIUM, IDENTIFICATION, ABORTION

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Chicago
Vandamme, Peter, Bruno Pot, and Karel Kersters. 1991. “Differentiation of Campylobacters and Campylobacter-like Organisms by Numerical Analysis of One-dimensional Electrophoretic Protein Patterns.” Systematic and Applied Microbiology 14 (1): 57–66.
APA
Vandamme, P., Pot, B., & Kersters, K. (1991). Differentiation of campylobacters and Campylobacter-like organisms by numerical analysis of one-dimensional electrophoretic protein patterns. SYSTEMATIC AND APPLIED MICROBIOLOGY, 14(1), 57–66.
Vancouver
1.
Vandamme P, Pot B, Kersters K. Differentiation of campylobacters and Campylobacter-like organisms by numerical analysis of one-dimensional electrophoretic protein patterns. SYSTEMATIC AND APPLIED MICROBIOLOGY. 1991;14(1):57–66.
MLA
Vandamme, Peter, Bruno Pot, and Karel Kersters. “Differentiation of Campylobacters and Campylobacter-like Organisms by Numerical Analysis of One-dimensional Electrophoretic Protein Patterns.” SYSTEMATIC AND APPLIED MICROBIOLOGY 14.1 (1991): 57–66. Print.
@article{222184,
  abstract     = {The suitability of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and computer-assisted comparison of the protein profiles for differentiation, identification and typing of campylobacters was evaluated. One hundred and fifty reference strains representing all Campylobacter, Helicobacter and {\textacutedbl}Flexi-spira{\textacutedbl} species were included in a single electrophoretic study and the influence of growth medium, growth conditions and laboratory-specific manipulations was investigated for several strains. The results indicate that this technique is very useful in the study of campylobacters, as (i) all species are clearly differentiated in one or more electrophoretic clusters or subclusters, (ii) the identification does not depend on the factors cited above and (iii) within several species, different electrophoretic types can be distinguished.},
  author       = {Vandamme, Peter and Pot, Bruno and Kersters, Karel},
  issn         = {0723-2020},
  journal      = {SYSTEMATIC AND APPLIED MICROBIOLOGY},
  keyword      = {CAMPYLOBACTER-LIKE ORGANISMS,CAMPYLOBACTER,TAXONOMY,EPIDEMIOLOGY,ELECTROPHORESIS OF PROTEINS,NUMERICAL ANALYSIS,POLYACRYLAMIDE-GEL ELECTROPHORESIS,CATALASE-NEGATIVE CAMPYLOBACTERS,FATTY-ACID COMPOSITION,THERMOPHILIC CAMPYLOBACTERS,BACTEROIDES-GRACILIS,DNA HOMOLOGY,SP-NOV,BACTERIUM,IDENTIFICATION,ABORTION},
  language     = {eng},
  number       = {1},
  pages        = {57--66},
  title        = {Differentiation of campylobacters and Campylobacter-like organisms by numerical analysis of one-dimensional electrophoretic protein patterns},
  volume       = {14},
  year         = {1991},
}