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Gene expression associated with water-stress adaptation of rice cells and identification of two genes as hsp 70 and ubiquitin

(1991) PHYSIOLOGIA PLANTARUM. 82(3). p.449-457
Author
Organization
Abstract
We have isolated polyethylene glycol (PEG)-adapted rice cells that can proliferate in a medium with 20% PEG as well as in a medium with 1% NaCl. The adapted-cells overproduce a set of proteins whose roles may be associated with water-stress adaptation. To isolate genes encoding these proteins, we differentially screened a cDNA library and obtained 5 cDNA clones which showed preferential hybridization to mRNA of adapted cells. The present paper describes the pattern of expression and the sequence analysis of these 5 genes. Sequence analysis of partial cDNAs indicates that two genes encode the 70 kDa heat shock protein and the ubiquitin but the identities of the other 3 are not known. The expression of all 5 genes fluctuates slightly during the growth cycle of the PEG-adapted cells grown in the control or in the PEG-containing medium. In contrast, gene expression in the parental cells fluctuates to a much greater extent and always begins with an enhanced expression during the first day after subculture. Sub-lethal concentrations of PEG or NaCl have no immediate effect on gene expression in parental cells but NaCl may have a long term effect in enhancing the expression of two genes after 5 days. Abscisic acid (ABA) has no effect on the expression of 4 genes but suppresses the expression of one gene. The roles of these genes in water-stress adaptation of plant cells are discussed.
Keywords
NACL, RNA, CARROT, INVITRO, ORYZA-SATIVA, POLYETHYLENE GLYCOL, RICE, VARIANT, WATER-STRESS, CULTURED TOBACCO CELLS, MAJOR HEAT-SHOCK, ABSCISIC-ACID, HIGHER-PLANTS, PROTEINS

Citation

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Chicago
Borkird, Chumpel, Chris Simoens, Raimundo Villarroel-Mandiola, and Marc Van Montagu. 1991. “Gene Expression Associated with Water-stress Adaptation of Rice Cells and Identification of Two Genes as Hsp 70 and Ubiquitin.” Physiologia Plantarum 82 (3): 449–457.
APA
Borkird, Chumpel, Simoens, C., Villarroel-Mandiola, R., & Van Montagu, M. (1991). Gene expression associated with water-stress adaptation of rice cells and identification of two genes as hsp 70 and ubiquitin. PHYSIOLOGIA PLANTARUM, 82(3), 449–457.
Vancouver
1.
Borkird C, Simoens C, Villarroel-Mandiola R, Van Montagu M. Gene expression associated with water-stress adaptation of rice cells and identification of two genes as hsp 70 and ubiquitin. PHYSIOLOGIA PLANTARUM. 1991;82(3):449–57.
MLA
Borkird, Chumpel, Chris Simoens, Raimundo Villarroel-Mandiola, et al. “Gene Expression Associated with Water-stress Adaptation of Rice Cells and Identification of Two Genes as Hsp 70 and Ubiquitin.” PHYSIOLOGIA PLANTARUM 82.3 (1991): 449–457. Print.
@article{221470,
  abstract     = {We have isolated polyethylene glycol (PEG)-adapted rice cells that can proliferate in a medium with 20% PEG as well as in a medium with 1% NaCl. The adapted-cells overproduce a set of proteins whose roles may be associated with water-stress adaptation. To isolate genes encoding these proteins, we differentially screened a cDNA library and obtained 5 cDNA clones which showed preferential hybridization to mRNA of adapted cells. The present paper describes the pattern of expression and the sequence analysis of these 5 genes. Sequence analysis of partial cDNAs indicates that two genes encode the 70 kDa heat shock protein and the ubiquitin but the identities of the other 3 are not known. The expression of all 5 genes fluctuates slightly during the growth cycle of the PEG-adapted cells grown in the control or in the PEG-containing medium. In contrast, gene expression in the parental cells fluctuates to a much greater extent and always begins with an enhanced expression during the first day after subculture. Sub-lethal concentrations of PEG or NaCl have no immediate effect on gene expression in parental cells but NaCl may have a long term effect in enhancing the expression of two genes after 5 days. Abscisic acid (ABA) has no effect on the expression of 4 genes but suppresses the expression of one gene. The roles of these genes in water-stress adaptation of plant cells are discussed.},
  author       = {Borkird, Chumpel and Simoens, Chris and Villarroel-Mandiola, Raimundo and Van Montagu, Marc},
  issn         = {0031-9317},
  journal      = {PHYSIOLOGIA PLANTARUM},
  keywords     = {NACL,RNA,CARROT,INVITRO,ORYZA-SATIVA,POLYETHYLENE GLYCOL,RICE,VARIANT,WATER-STRESS,CULTURED TOBACCO CELLS,MAJOR HEAT-SHOCK,ABSCISIC-ACID,HIGHER-PLANTS,PROTEINS},
  language     = {eng},
  number       = {3},
  pages        = {449--457},
  title        = {Gene expression associated with water-stress adaptation of rice cells and identification of two genes as hsp 70 and ubiquitin},
  url          = {http://dx.doi.org/10.1111/j.1399-3054.1991.tb02931.x},
  volume       = {82},
  year         = {1991},
}

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