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Distribution of the rDNA and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana

Serge Bauwens UGent, Patric Van Oostveldt UGent, Gilbert Engler UGent and Marc Van Montagu UGent (1991) CHROMOSOMA. 101(1). p.41-48
abstract
The distribution of the ribosomal RNA (rRNA) genes and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana was studied for the first time through non-isotopic in situ hybridization and luminescence digital imaging microscopy. Each of the three classes of highly repetitive DNA exhibited a characteristic hybridization pattern, and one class was seen to be primarily localized on two chromocentres, which would allow it to distinguish a particular chromosome. The rDNA was consistently localized on the two largest chromocentres and on one or two smaller chromocentres. A limited number of nuclei exhibited more than four labelled chromocentres, indicative of either polypoidy or differential amplification of the rDNA. In nuclei where the nucleolus could be clearly observed, the nucleolar associated chromocentres (NACs) were seen to be labelled by the ribosomal DNA (rDNA) probe.
Please use this url to cite or link to this publication:
author
organization
alternative title
Distribution of the rDNA and 3 classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana
year
type
journalArticle (original)
publication status
published
subject
keyword
DROSOPHILA-MELANOGASTER, OPTICAL MICROSCOPY, SPATIAL-ORGANIZATION, FLUORESCENCE INSITU HYBRIDIZATION, 3-DIMENSIONAL ORGANIZATION, CHROMOSOME DOMAINS, SEQUENCE, POSITION, SITES
journal title
CHROMOSOMA
Chromosoma
volume
101
issue
1
pages
41 - 48
Web of Science type
Article
ISSN
0009-5915
DOI
10.1007/BF00360685
language
English
UGent publication?
yes
classification
A1
id
221460
handle
http://hdl.handle.net/1854/LU-221460
date created
2004-05-24 13:24:00
date last changed
2016-12-19 15:38:50
@article{221460,
  abstract     = {The distribution of the ribosomal RNA (rRNA) genes and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana was studied for the first time through non-isotopic in situ hybridization and luminescence digital imaging microscopy. Each of the three classes of highly repetitive DNA exhibited a characteristic hybridization pattern, and one class was seen to be primarily localized on two chromocentres, which would allow it to distinguish a particular chromosome. The rDNA was consistently localized on the two largest chromocentres and on one or two smaller chromocentres. A limited number of nuclei exhibited more than four labelled chromocentres, indicative of either polypoidy or differential amplification of the rDNA. In nuclei where the nucleolus could be clearly observed, the nucleolar associated chromocentres (NACs) were seen to be labelled by the ribosomal DNA (rDNA) probe.},
  author       = {Bauwens, Serge and Van Oostveldt, Patric and Engler, Gilbert and Van Montagu, Marc},
  issn         = {0009-5915},
  journal      = {CHROMOSOMA},
  keyword      = {DROSOPHILA-MELANOGASTER,OPTICAL MICROSCOPY,SPATIAL-ORGANIZATION,FLUORESCENCE INSITU HYBRIDIZATION,3-DIMENSIONAL ORGANIZATION,CHROMOSOME DOMAINS,SEQUENCE,POSITION,SITES},
  language     = {eng},
  number       = {1},
  pages        = {41--48},
  title        = {Distribution of the rDNA and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1007/BF00360685},
  volume       = {101},
  year         = {1991},
}

Chicago
Bauwens, Serge, Patric Van Oostveldt, Gilbert Engler, and Marc Van Montagu. 1991. “Distribution of the rDNA and Three Classes of Highly Repetitive DNA in the Chromatin of Interphase Nuclei of Arabidopsis Thaliana.” Chromosoma 101 (1): 41–48.
APA
Bauwens, Serge, Van Oostveldt, P., Engler, G., & Van Montagu, M. (1991). Distribution of the rDNA and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana. CHROMOSOMA, 101(1), 41–48.
Vancouver
1.
Bauwens S, Van Oostveldt P, Engler G, Van Montagu M. Distribution of the rDNA and three classes of highly repetitive DNA in the chromatin of interphase nuclei of Arabidopsis thaliana. CHROMOSOMA. 1991;101(1):41–8.
MLA
Bauwens, Serge, Patric Van Oostveldt, Gilbert Engler, et al. “Distribution of the rDNA and Three Classes of Highly Repetitive DNA in the Chromatin of Interphase Nuclei of Arabidopsis Thaliana.” CHROMOSOMA 101.1 (1991): 41–48. Print.