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The influence of chronic morphine on the canine cerebral 5-HT2A receptor availability measured with SPECT

Antita Adriaens (UGent) , Ingeborgh Polis (UGent) , Simon Vermeire (UGent) , Eva Vandermeulen (UGent) , Tim Waelbers (UGent) , Jos Eersels, André Dobbeleir, Luc Duchateau (UGent) , Sylvia Van Dorpe (UGent) , Bart De Spiegeleer (UGent) , et al.
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Abstract
A major drawback from opioid therapy is tolerance development. Drugs acting on the serotonergic (5-HT) system have been studied to prevent this problem. The study examines the effect of chronic opioids on cerebral 5-HT2A receptors. Brain 5-HT2A receptor availability was estimated in seven healthy five-year-old female neutered Beagle dogs pre and post chronic morphine treatment (oral sustained release morphine 20 mg twice daily for ten days) with 123I-5-I-R-91150 (a 5-HT2A selective radioligand) and Single Photon Emission Computed Tomography (SPECT). Before and on the last day of morphine treatment, SPECT scans were performed 90 minutes after 123I-5-I-R91150 injection. Dogs were premedicated with dexmedetomidine (375 µg m-2 body surface area, IM). Anesthesia was induced with propofol (2.80 ± 0.63 mg kg-1 IV) and maintained with isoflurane in oxygen. 5-HT2A receptor binding indices (BI) for the frontal, parietal, temporal and occipital cortex and the subcortical region were calculated by semiquantification with the cerebellum (devoid of 5-HT2A receptors) as a reference region. Data were analyzed by mixed-model with treatment as fixed effect and dog as random effect. Chronic morphine treatment significantly (p ≤ 0.05) lowered 5-HT2A BI’s in the right and left frontal cortex, the right and left temporal cortex, the right and left parietal cortex, and the subcortical region. The decreased cerebral 5-HT2A receptor availability following chronic morphine treatment suggests an interaction between the opioid and serotonergic system, the exact nature of this interaction remains to be elucidated.

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Chicago
Adriaens, Antita, Ingeborgh Polis, Simon Vermeire, Eva Vandermeulen, Tim Waelbers, Jos Eersels, André Dobbeleir, et al. 2012. “The Influence of Chronic Morphine on the Canine Cerebral 5-HT2A Receptor Availability Measured with SPECT.” In AVA Spring Meeting Davos 2012.
APA
Adriaens, Antita, Polis, I., Vermeire, S., Vandermeulen, E., Waelbers, T., Eersels, J., Dobbeleir, A., et al. (2012). The influence of chronic morphine on the canine cerebral 5-HT2A receptor availability measured with SPECT. AVA spring meeting Davos 2012. Presented at the AVA 2012 Spring Meeting.
Vancouver
1.
Adriaens A, Polis I, Vermeire S, Vandermeulen E, Waelbers T, Eersels J, et al. The influence of chronic morphine on the canine cerebral 5-HT2A receptor availability measured with SPECT. AVA spring meeting Davos 2012. 2012.
MLA
Adriaens, Antita, Ingeborgh Polis, Simon Vermeire, et al. “The Influence of Chronic Morphine on the Canine Cerebral 5-HT2A Receptor Availability Measured with SPECT.” AVA Spring Meeting Davos 2012. 2012. Print.
@inproceedings{2154782,
  abstract     = {A major drawback from opioid therapy is tolerance development. Drugs acting on the serotonergic (5-HT) system have been studied to prevent this problem. The study examines the effect of chronic opioids on cerebral 5-HT2A receptors.
Brain 5-HT2A receptor availability was estimated in seven healthy five-year-old female neutered Beagle dogs pre and post chronic morphine treatment (oral sustained release morphine 20 mg twice daily for ten days) with 123I-5-I-R-91150 (a 5-HT2A selective radioligand) and Single Photon Emission Computed Tomography (SPECT). Before and on the last day of morphine treatment, SPECT scans were performed 90 minutes after 123I-5-I-R91150 injection. Dogs were premedicated with dexmedetomidine (375 µg m-2 body surface area, IM). Anesthesia was induced with propofol (2.80 ± 0.63 mg kg-1 IV) and maintained with isoflurane in oxygen. 5-HT2A receptor binding indices (BI) for the frontal, parietal, temporal and occipital cortex and the subcortical region were calculated by semiquantification with the cerebellum (devoid of 5-HT2A receptors) as a reference region. Data were analyzed by mixed-model with treatment as fixed effect and dog as random effect.
Chronic morphine treatment significantly (p ≤ 0.05) lowered 5-HT2A BI’s in the right and left frontal cortex, the right and left temporal cortex, the right  and left parietal cortex, and the subcortical region.
The decreased cerebral 5-HT2A receptor availability following chronic morphine treatment suggests an interaction between the opioid and serotonergic system, the exact nature of this interaction remains to be elucidated.},
  author       = {Adriaens, Antita and Polis, Ingeborgh and Vermeire, Simon and Vandermeulen, Eva and Waelbers, Tim and Eersels, Jos and Dobbeleir, André and Duchateau, Luc and Van Dorpe, Sylvia and De Spiegeleer, Bart and Peremans, Kathelijne},
  booktitle    = {AVA spring meeting Davos 2012},
  language     = {eng},
  location     = {Davos, Switserland},
  title        = {The influence of chronic morphine on the canine cerebral 5-HT2A receptor availability measured with SPECT},
  year         = {2012},
}