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Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells

Mukund Zambre UGent, Nancy Terryn UGent, Janniek De Clercq UGent, Sylvie De Buck UGent, Willy Dillen UGent, Marc Van Montagu UGent, Dominique Van Der Straeten UGent and Geert Angenon UGent (2003) PLANTA. 216(4). p.580-586
abstract
Light conditions during Agrobacterium-based plant transformation, the most routinely used method in plant genetic engineering, differ widely and, to our knowledge, have not been studied systematically in relation to transformation efficiency. Here, light effects were examined in two already optimized transformation procedures: coculture of Agrobacterium tumefaciens with callus from two genotypes of the crop plant Phaseolus acutifolius (tepary bean) and coculture of root segments from two ecotypes of Arabidopsis thaliana. Except for the light conditions during coculture, all steps followed established procedures. Coculture was done either under continuous darkness, under a commonly used photoperiod of 16 h light/8 h darkness or under continuous light. beta-glucuronidase (GUS) production due to the transient expression of an intron-containing uidA gene in the binary vector was used to evaluate T-DNA transfer. In all situations, uidA expression correlated highly and positively with the light period used during coculture; it was inhibited severely by darkness and enhanced more under continuous light than under a 16 h light/8 h dark photoperiod. The promotive effect of light was observed with Agrobacterium strains harboring either a nopaline-, an octopine- or an agropine/succinamopine-type nononcogenic helper Ti plasmid. The observed positive effect of light has obvious implications for developing and improving transient and stable transformation protocols, specifically those involving dark coculture conditions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SHOOT ORGANOGENESIS, EXPRESSION, IN-VITRO, REGENERATION, COMMON, PHASEOLUS-ACUTIFOLIUS, MEDIATED TRANSFORMATION, ARABIDOPSIS-THALIANA, T-DNA TRANSFER, THALIANA ROOT EXPLANTS, transformation, Phaseolus, light, coculture, Agrobacterium, Arabidopsis
journal title
PLANTA
Planta
volume
216
issue
4
pages
580 - 586
Web of Science type
Article
Web of Science id
000181345700005
JCR category
PLANT SCIENCES
JCR impact factor
3.053 (2003)
JCR rank
15/136 (2003)
JCR quartile
1 (2003)
ISSN
0032-0935
DOI
10.1007/s00425-002-0914-2
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
215363
handle
http://hdl.handle.net/1854/LU-215363
date created
2004-05-10 18:41:00
date last changed
2013-10-17 15:08:12
@article{215363,
  abstract     = {Light conditions during Agrobacterium-based plant transformation, the most routinely used method in plant genetic engineering, differ widely and, to our knowledge, have not been studied systematically in relation to transformation efficiency. Here, light effects were examined in two already optimized transformation procedures: coculture of Agrobacterium tumefaciens with callus from two genotypes of the crop plant Phaseolus acutifolius (tepary bean) and coculture of root segments from two ecotypes of Arabidopsis thaliana. Except for the light conditions during coculture, all steps followed established procedures. Coculture was done either under continuous darkness, under a commonly used photoperiod of 16 h light/8 h darkness or under continuous light. beta-glucuronidase (GUS) production due to the transient expression of an intron-containing uidA gene in the binary vector was used to evaluate T-DNA transfer. In all situations, uidA expression correlated highly and positively with the light period used during coculture; it was inhibited severely by darkness and enhanced more under continuous light than under a 16 h light/8 h dark photoperiod. The promotive effect of light was observed with Agrobacterium strains harboring either a nopaline-, an octopine- or an agropine/succinamopine-type nononcogenic helper Ti plasmid. The observed positive effect of light has obvious implications for developing and improving transient and stable transformation protocols, specifically those involving dark coculture conditions.},
  author       = {Zambre, Mukund and Terryn, Nancy and De Clercq, Janniek and De Buck, Sylvie and Dillen, Willy and Van Montagu, Marc and Van Der Straeten, Dominique and Angenon, Geert},
  issn         = {0032-0935},
  journal      = {PLANTA},
  keyword      = {SHOOT ORGANOGENESIS,EXPRESSION,IN-VITRO,REGENERATION,COMMON,PHASEOLUS-ACUTIFOLIUS,MEDIATED TRANSFORMATION,ARABIDOPSIS-THALIANA,T-DNA TRANSFER,THALIANA ROOT EXPLANTS,transformation,Phaseolus,light,coculture,Agrobacterium,Arabidopsis},
  language     = {eng},
  number       = {4},
  pages        = {580--586},
  title        = {Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells},
  url          = {http://dx.doi.org/10.1007/s00425-002-0914-2},
  volume       = {216},
  year         = {2003},
}

Chicago
Zambre, Mukund, Nancy Terryn, Janniek De Clercq, Sylvie De Buck, Willy Dillen, Marc Van Montagu, Dominique Van Der Straeten, and Geert Angenon. 2003. “Light Strongly Promotes Gene Transfer from Agrobacterium Tumefaciens to Plant Cells.” Planta 216 (4): 580–586.
APA
Zambre, M., Terryn, N., De Clercq, J., De Buck, S., Dillen, W., Van Montagu, M., Van Der Straeten, D., et al. (2003). Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells. PLANTA, 216(4), 580–586.
Vancouver
1.
Zambre M, Terryn N, De Clercq J, De Buck S, Dillen W, Van Montagu M, et al. Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells. PLANTA. 2003;216(4):580–6.
MLA
Zambre, Mukund, Nancy Terryn, Janniek De Clercq, et al. “Light Strongly Promotes Gene Transfer from Agrobacterium Tumefaciens to Plant Cells.” PLANTA 216.4 (2003): 580–586. Print.