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Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation

Kobe Vlieghe UGent, Marnik Vuylsteke UGent, Kobe Florquin UGent, Stephane Rombauts UGent, Sara Maes UGent, Sandra Ormenese, Paul Van Hummelen, Yves Van de Peer UGent, Dirk Inzé UGent and Lieven De Veylder UGent (2003) JOURNAL OF CELL SCIENCE. 116(20). p.4249-4259
abstract
Previously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CLONING, IDENTIFICATION, XYLOGLUCAN, PROMOTER, EXPRESSION, BINDING PROTEIN, REGULATORY ELEMENTS, TRANSCRIPTION FACTORS, E2F SITES, ARABIDOPSIS-THALIANA, nitrogen assimilation, microarray, E2F, Arabidopsis thaliana, cell cycle
journal title
JOURNAL OF CELL SCIENCE
J. Cell Sci.
volume
116
issue
20
pages
4249 - 4259
Web of Science type
Article
Web of Science id
000187395200019
JCR category
CELL BIOLOGY
JCR impact factor
7.25 (2003)
JCR rank
21/154 (2003)
JCR quartile
1 (2003)
ISSN
0021-9533
DOI
10.1242/jcs.00715
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
215354
handle
http://hdl.handle.net/1854/LU-215354
date created
2004-05-10 18:38:00
date last changed
2016-12-19 15:40:22
@article{215354,
  abstract     = {Previously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.},
  author       = {Vlieghe, Kobe and Vuylsteke, Marnik and Florquin, Kobe and Rombauts, Stephane and Maes, Sara and Ormenese, Sandra and Van Hummelen, Paul and Van de Peer, Yves and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0021-9533},
  journal      = {JOURNAL OF CELL SCIENCE},
  keyword      = {CLONING,IDENTIFICATION,XYLOGLUCAN,PROMOTER,EXPRESSION,BINDING PROTEIN,REGULATORY ELEMENTS,TRANSCRIPTION FACTORS,E2F SITES,ARABIDOPSIS-THALIANA,nitrogen assimilation,microarray,E2F,Arabidopsis thaliana,cell cycle},
  language     = {eng},
  number       = {20},
  pages        = {4249--4259},
  title        = {Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation},
  url          = {http://dx.doi.org/10.1242/jcs.00715},
  volume       = {116},
  year         = {2003},
}

Chicago
Vlieghe, Kobe, Marnik Vuylsteke, Kobe Florquin, Stephane Rombauts, Sara Maes, Sandra Ormenese, Paul Van Hummelen, Yves Van de Peer, Dirk Inzé, and Lieven De Veylder. 2003. “Microarray Analysis of E2Fa-DPa-overexpressing Plants Uncovers a Cross-talking Genetic Network Between DNA Replication and Nitrogen Assimilation.” Journal of Cell Science 116 (20): 4249–4259.
APA
Vlieghe, Kobe, Vuylsteke, M., Florquin, K., Rombauts, S., Maes, S., Ormenese, S., Van Hummelen, P., et al. (2003). Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. JOURNAL OF CELL SCIENCE, 116(20), 4249–4259.
Vancouver
1.
Vlieghe K, Vuylsteke M, Florquin K, Rombauts S, Maes S, Ormenese S, et al. Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. JOURNAL OF CELL SCIENCE. 2003;116(20):4249–59.
MLA
Vlieghe, Kobe, Marnik Vuylsteke, Kobe Florquin, et al. “Microarray Analysis of E2Fa-DPa-overexpressing Plants Uncovers a Cross-talking Genetic Network Between DNA Replication and Nitrogen Assimilation.” JOURNAL OF CELL SCIENCE 116.20 (2003): 4249–4259. Print.