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Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation

(2003) JOURNAL OF CELL SCIENCE. 116(20). p.4249-4259
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Abstract
Previously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.
Keywords
CLONING, IDENTIFICATION, XYLOGLUCAN, PROMOTER, EXPRESSION, BINDING PROTEIN, REGULATORY ELEMENTS, TRANSCRIPTION FACTORS, E2F SITES, ARABIDOPSIS-THALIANA, nitrogen assimilation, microarray, E2F, Arabidopsis thaliana, cell cycle

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Chicago
Vlieghe, Kobe, Marnik Vuylsteke, Kobe Florquin, Stephane Rombauts, Sara Maes, Sandra Ormenese, Paul Van Hummelen, Yves Van de Peer, Dirk Inzé, and Lieven De Veylder. 2003. “Microarray Analysis of E2Fa-DPa-overexpressing Plants Uncovers a Cross-talking Genetic Network Between DNA Replication and Nitrogen Assimilation.” Journal of Cell Science 116 (20): 4249–4259.
APA
Vlieghe, Kobe, Vuylsteke, M., Florquin, K., Rombauts, S., Maes, S., Ormenese, S., Van Hummelen, P., et al. (2003). Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. JOURNAL OF CELL SCIENCE, 116(20), 4249–4259.
Vancouver
1.
Vlieghe K, Vuylsteke M, Florquin K, Rombauts S, Maes S, Ormenese S, et al. Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation. JOURNAL OF CELL SCIENCE. 2003;116(20):4249–59.
MLA
Vlieghe, Kobe, Marnik Vuylsteke, Kobe Florquin, et al. “Microarray Analysis of E2Fa-DPa-overexpressing Plants Uncovers a Cross-talking Genetic Network Between DNA Replication and Nitrogen Assimilation.” JOURNAL OF CELL SCIENCE 116.20 (2003): 4249–4259. Print.
@article{215354,
  abstract     = {Previously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.},
  author       = {Vlieghe, Kobe and Vuylsteke, Marnik and Florquin, Kobe and Rombauts, Stephane and Maes, Sara and Ormenese, Sandra and Van Hummelen, Paul and Van de Peer, Yves and Inz{\'e}, Dirk and De Veylder, Lieven},
  issn         = {0021-9533},
  journal      = {JOURNAL OF CELL SCIENCE},
  keyword      = {CLONING,IDENTIFICATION,XYLOGLUCAN,PROMOTER,EXPRESSION,BINDING PROTEIN,REGULATORY ELEMENTS,TRANSCRIPTION FACTORS,E2F SITES,ARABIDOPSIS-THALIANA,nitrogen assimilation,microarray,E2F,Arabidopsis thaliana,cell cycle},
  language     = {eng},
  number       = {20},
  pages        = {4249--4259},
  title        = {Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation},
  url          = {http://dx.doi.org/10.1242/jcs.00715},
  volume       = {116},
  year         = {2003},
}

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