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Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin

Davy Vanden Broeck UGent, Albert R Lagrou and Marc JS De Wolf (2007) ACTA BIOCHIMICA POLONICA. 54(4). p.757-767
abstract
The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y neuroblastoma cells and Madin-Darby canine kidney cells. Suppression of the clathrin-mediated endocytic pathway by classical biochemical procedures, like intracellular acidification and potassium depletion, inhibited cholera toxin uptake by up to about 50% as well as its ability to raise intracellular levels of cAMP. Also prior exposure of these cell types to the cationic amphiphilic drug chlorpromazine reduced the functional uptake of cholera toxin, even to a greater extent. These effects were dose- and cell type-dependent, suggesting an involvement of clathrin-mediated endocytosis in the functional uptake of cholera toxin. For a more straightforward approach to study the role of the clathrin-mediated uptake in the internalization of cholera toxin, a Caco-2(eps-) cell line was exploited. These Caco2(eps-) cells constitutively suppress the expression of epsin, an essential accessory protein of clathrin-mediated endocytosis, thereby selectively blocking this internalization route. CT uptake was found to be reduced by over 60% in Caco-2(eps-) paralleled by a diminished ability of CT to raise the level of cAMP. The data presented suggest that the clathrin-mediated uptake route fulfils an important role in the functional internalization of cholera toxin in several cell types.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
internalization, chlorpromazine, siRNA, clathrin-mediated endocytosis, cholera toxin, COATED PIT FORMATION, PLASMA-MEMBRANE, CAVEOLAE, INTERNALIZATION, MICRODOMAINS, CHOLESTEROL, DEPLETION, BLOCKS, CYCLODEXTRIN, TRAFFICKING
journal title
ACTA BIOCHIMICA POLONICA
Acta Biochim. Pol.
volume
54
issue
4
pages
757 - 767
Web of Science type
Article
Web of Science id
000252047200010
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
1.261 (2007)
JCR rank
218/260 (2007)
JCR quartile
4 (2007)
ISSN
0001-527X
language
English
UGent publication?
no
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2151457
handle
http://hdl.handle.net/1854/LU-2151457
date created
2012-06-13 15:01:44
date last changed
2016-12-19 15:44:35
@article{2151457,
  abstract     = {The involvement of the clathrin-mediated endocytic internalization route in the uptake of cholera toxin (CT) was investigated using different cell lines, including the human intestinal Caco-2 and T84 cell lines, green monkey Vero cells, SH-SY5Y neuroblastoma cells and Madin-Darby canine kidney cells. Suppression of the clathrin-mediated endocytic pathway by classical biochemical procedures, like intracellular acidification and potassium depletion, inhibited cholera toxin uptake by up to about 50\% as well as its ability to raise intracellular levels of cAMP. Also prior exposure of these cell types to the cationic amphiphilic drug chlorpromazine reduced the functional uptake of cholera toxin, even to a greater extent. These effects were dose- and cell type-dependent, suggesting an involvement of clathrin-mediated endocytosis in the functional uptake of cholera toxin. For a more straightforward approach to study the role of the clathrin-mediated uptake in the internalization of cholera toxin, a Caco-2(eps-) cell line was exploited. These Caco2(eps-) cells constitutively suppress the expression of epsin, an essential accessory protein of clathrin-mediated endocytosis, thereby selectively blocking this internalization route. CT uptake was found to be reduced by over 60\% in Caco-2(eps-) paralleled by a diminished ability of CT to raise the level of cAMP. The data presented suggest that the clathrin-mediated uptake route fulfils an important role in the functional internalization of cholera toxin in several cell types.},
  author       = {Vanden Broeck, Davy and Lagrou, Albert R and De Wolf, Marc JS},
  issn         = {0001-527X},
  journal      = {ACTA BIOCHIMICA POLONICA},
  keyword      = {internalization,chlorpromazine,siRNA,clathrin-mediated endocytosis,cholera toxin,COATED PIT FORMATION,PLASMA-MEMBRANE,CAVEOLAE,INTERNALIZATION,MICRODOMAINS,CHOLESTEROL,DEPLETION,BLOCKS,CYCLODEXTRIN,TRAFFICKING},
  language     = {eng},
  number       = {4},
  pages        = {757--767},
  title        = {Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin},
  volume       = {54},
  year         = {2007},
}

Chicago
Vanden Broeck, Davy, Albert R Lagrou, and Marc JS De Wolf. 2007. “Distinct Role of Clathrin-mediated Endocytosis in the Functional Uptake of Cholera Toxin.” Acta Biochimica Polonica 54 (4): 757–767.
APA
Vanden Broeck, D., Lagrou, A. R., & De Wolf, M. J. (2007). Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin. ACTA BIOCHIMICA POLONICA, 54(4), 757–767.
Vancouver
1.
Vanden Broeck D, Lagrou AR, De Wolf MJ. Distinct role of clathrin-mediated endocytosis in the functional uptake of cholera toxin. ACTA BIOCHIMICA POLONICA. 2007;54(4):757–67.
MLA
Vanden Broeck, Davy, Albert R Lagrou, and Marc JS De Wolf. “Distinct Role of Clathrin-mediated Endocytosis in the Functional Uptake of Cholera Toxin.” ACTA BIOCHIMICA POLONICA 54.4 (2007): 757–767. Print.