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Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations

(2003) NUCLEIC ACIDS RESEARCH. 31(15). p.4401-4409
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Abstract
Frameshift mutations generally result in loss-of-function changes since they drastically alter the protein sequence downstream of the frameshift site, besides creating premature stop codons. Here we present data suggesting that frameshift mutations in the C-terminal domain of specific ancestral MADS-box genes may have contributed to the structural and functional divergence of the MADS-box gene family. We have identified putative frameshift mutations in the conserved C-terminal motifs of the B-function DEF/AP3 subfamily, the A-function SQUA/AP1 subfamily and the E-function AGL2 subfamily, which are all involved in the specification of organ identity during flower development. The newly evolved C-terminal motifs are highly conserved, suggesting a de novo generation of functionality. Interestingly, since the new C-terminal motifs in the A- and B-function subfamilies are only found in higher eudicotyledonous flowering plants, the emergence of these two C-terminal changes coincides with the origin of a highly standardized floral structure. We speculate that the frameshift mutations described here are examples of co-evolution of the different components of a single transcription factor complex. 3' terminal frameshift mutations might provide an important but so far unrecognized mechanism to generate novel functional C-terminal motifs instrumental to the functional diversification of transcription factor families.
Keywords
FLOWER DEVELOPMENT, ANTIRRHINUM-MAJUS, HOMEOTIC GENES, TRANSCRIPTION FACTORS, ORGAN IDENTITY, ARABIDOPSIS, DOMAIN, PROTEINS, FAMILY, APETALA3

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MLA
Vandenbussche, Michiel, Günter Theißen, Yves Van de Peer, et al. “Structural Diversification and Neo-functionalization During Floral MADS-box Gene Evolution by C-terminal Frameshift Mutations.” NUCLEIC ACIDS RESEARCH 31.15 (2003): 4401–4409. Print.
APA
Vandenbussche, Michiel, Theißen, G., Van de Peer, Y., & Gerats, T. (2003). Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations. NUCLEIC ACIDS RESEARCH, 31(15), 4401–4409.
Chicago author-date
Vandenbussche, Michiel, Günter Theißen, Yves Van de Peer, and Tom Gerats. 2003. “Structural Diversification and Neo-functionalization During Floral MADS-box Gene Evolution by C-terminal Frameshift Mutations.” Nucleic Acids Research 31 (15): 4401–4409.
Chicago author-date (all authors)
Vandenbussche, Michiel, Günter Theißen, Yves Van de Peer, and Tom Gerats. 2003. “Structural Diversification and Neo-functionalization During Floral MADS-box Gene Evolution by C-terminal Frameshift Mutations.” Nucleic Acids Research 31 (15): 4401–4409.
Vancouver
1.
Vandenbussche M, Theißen G, Van de Peer Y, Gerats T. Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations. NUCLEIC ACIDS RESEARCH. 2003;31(15):4401–9.
IEEE
[1]
M. Vandenbussche, G. Theißen, Y. Van de Peer, and T. Gerats, “Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations,” NUCLEIC ACIDS RESEARCH, vol. 31, no. 15, pp. 4401–4409, 2003.
@article{214715,
  abstract     = {{Frameshift mutations generally result in loss-of-function changes since they drastically alter the protein sequence downstream of the frameshift site, besides creating premature stop codons. Here we present data suggesting that frameshift mutations in the C-terminal domain of specific ancestral MADS-box genes may have contributed to the structural and functional divergence of the MADS-box gene family. We have identified putative frameshift mutations in the conserved C-terminal motifs of the B-function DEF/AP3 subfamily, the A-function SQUA/AP1 subfamily and the E-function AGL2 subfamily, which are all involved in the specification of organ identity during flower development. The newly evolved C-terminal motifs are highly conserved, suggesting a de novo generation of functionality. Interestingly, since the new C-terminal motifs in the A- and B-function subfamilies are only found in higher eudicotyledonous flowering plants, the emergence of these two C-terminal changes coincides with the origin of a highly standardized floral structure. We speculate that the frameshift mutations described here are examples of co-evolution of the different components of a single transcription factor complex. 3' terminal frameshift mutations might provide an important but so far unrecognized mechanism to generate novel functional C-terminal motifs instrumental to the functional diversification of transcription factor families.}},
  author       = {{Vandenbussche, Michiel and Theißen, Günter and Van de Peer, Yves and Gerats, Tom}},
  issn         = {{0305-1048}},
  journal      = {{NUCLEIC ACIDS RESEARCH}},
  keywords     = {{FLOWER DEVELOPMENT,ANTIRRHINUM-MAJUS,HOMEOTIC GENES,TRANSCRIPTION FACTORS,ORGAN IDENTITY,ARABIDOPSIS,DOMAIN,PROTEINS,FAMILY,APETALA3}},
  language     = {{eng}},
  number       = {{15}},
  pages        = {{4401--4409}},
  title        = {{Structural diversification and neo-functionalization during floral MADS-box gene evolution by C-terminal frameshift mutations}},
  url          = {{http://dx.doi.org/10.1093/nar/gkg642}},
  volume       = {{31}},
  year         = {{2003}},
}

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