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Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches: B, C, and D floral organ identity functions require SEPALLATA-like MADS box genes in petunia

(2003) PLANT CELL. 15(11). p.2680-2693
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Abstract
We have initiated a systematic functional analysis of the MAIDS box, intervening region, K domain, C domain-type MAIDS box gene family in petunia. The starting point for this has been a reverse-genetics approach, aiming to select for transposon insertions into any MAIDS box gene. We have developed and applied a family signature insertion screening protocol that is highly suited for this purpose, resulting in the isolation of 32 insertion mutants in 20 different MAIDS box genes. In addition, we identified three more MAIDS box gene insertion mutants using a candidate-gene approach. The defined insertion lines provide a sound foundation for a systematic functional analysis of the MAIDS box gene family in petunia. Here, we focus on the analysis of Floral Binding Protein2 (FBP2) and FBP5 genes that encode the E-function, which in Arabidopsis has been shown to be required for B and C floral organ identity functions. fbp2 mutants display sepaloid petals and ectopic inflorescences originating from the third floral whorl, whereas fbp5 mutants appear as wild type. In fbp2 fbp5 double mutants, reversion of floral organs to leaf-like organs is increased further. Strikingly, ovules are replaced by leaf-like structures in the carpel, indicating that in addition to the B- and C-functions, the D-function, which specifies ovule development, requires E-function activity. Finally, we compare our data with results obtained using cosuppression approaches and conclude that the latter might be less suited for assigning functions to individual members of the MAIDS box gene family.
Keywords
PLANTS, HOMEOTIC GENES, ARABIDOPSIS, SEED DEVELOPMENT, OVULE DEVELOPMENT, MERISTEM IDENTITY, ANTIRRHINUM-MAJUS, PROTEIN-PROTEIN INTERACTIONS, TRANSCRIPTION FACTOR FAMILY, FLOWER DEVELOPMENT

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MLA
Vandenbussche, Michiel, Johannes Zethof, Erik Souer, et al. “Toward the Analysis of the Petunia MADS Box Gene Family by Reverse and Forward Transposon Insertion Mutagenesis Approaches: B, C, and D Floral Organ Identity Functions Require SEPALLATA-like MADS Box Genes in Petunia.” PLANT CELL 15.11 (2003): 2680–2693. Print.
APA
Vandenbussche, Michiel, Zethof, J., Souer, E., Koes, R., Tornielli, G. B., Pezzotti, M., Ferrario, S., et al. (2003). Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches: B, C, and D floral organ identity functions require SEPALLATA-like MADS box genes in petunia. PLANT CELL, 15(11), 2680–2693.
Chicago author-date
Vandenbussche, Michiel, Johannes Zethof, Erik Souer, Ronald Koes, Giovanni B Tornielli, Mario Pezzotti, Silvia Ferrario, Gerco C Angenent, and Tom Gerats. 2003. “Toward the Analysis of the Petunia MADS Box Gene Family by Reverse and Forward Transposon Insertion Mutagenesis Approaches: B, C, and D Floral Organ Identity Functions Require SEPALLATA-like MADS Box Genes in Petunia.” Plant Cell 15 (11): 2680–2693.
Chicago author-date (all authors)
Vandenbussche, Michiel, Johannes Zethof, Erik Souer, Ronald Koes, Giovanni B Tornielli, Mario Pezzotti, Silvia Ferrario, Gerco C Angenent, and Tom Gerats. 2003. “Toward the Analysis of the Petunia MADS Box Gene Family by Reverse and Forward Transposon Insertion Mutagenesis Approaches: B, C, and D Floral Organ Identity Functions Require SEPALLATA-like MADS Box Genes in Petunia.” Plant Cell 15 (11): 2680–2693.
Vancouver
1.
Vandenbussche M, Zethof J, Souer E, Koes R, Tornielli GB, Pezzotti M, et al. Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches: B, C, and D floral organ identity functions require SEPALLATA-like MADS box genes in petunia. PLANT CELL. 2003;15(11):2680–93.
IEEE
[1]
M. Vandenbussche et al., “Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches: B, C, and D floral organ identity functions require SEPALLATA-like MADS box genes in petunia,” PLANT CELL, vol. 15, no. 11, pp. 2680–2693, 2003.
@article{214705,
  abstract     = {We have initiated a systematic functional analysis of the MAIDS box, intervening region, K domain, C domain-type MAIDS box gene family in petunia. The starting point for this has been a reverse-genetics approach, aiming to select for transposon insertions into any MAIDS box gene. We have developed and applied a family signature insertion screening protocol that is highly suited for this purpose, resulting in the isolation of 32 insertion mutants in 20 different MAIDS box genes. In addition, we identified three more MAIDS box gene insertion mutants using a candidate-gene approach. The defined insertion lines provide a sound foundation for a systematic functional analysis of the MAIDS box gene family in petunia. Here, we focus on the analysis of Floral Binding Protein2 (FBP2) and FBP5 genes that encode the E-function, which in Arabidopsis has been shown to be required for B and C floral organ identity functions. fbp2 mutants display sepaloid petals and ectopic inflorescences originating from the third floral whorl, whereas fbp5 mutants appear as wild type. In fbp2 fbp5 double mutants, reversion of floral organs to leaf-like organs is increased further. Strikingly, ovules are replaced by leaf-like structures in the carpel, indicating that in addition to the B- and C-functions, the D-function, which specifies ovule development, requires E-function activity. Finally, we compare our data with results obtained using cosuppression approaches and conclude that the latter might be less suited for assigning functions to individual members of the MAIDS box gene family.},
  author       = {Vandenbussche, Michiel and Zethof, Johannes and Souer, Erik and Koes, Ronald and Tornielli, Giovanni B and Pezzotti, Mario and Ferrario, Silvia and Angenent, Gerco C and Gerats, Tom},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keywords     = {PLANTS,HOMEOTIC GENES,ARABIDOPSIS,SEED DEVELOPMENT,OVULE DEVELOPMENT,MERISTEM IDENTITY,ANTIRRHINUM-MAJUS,PROTEIN-PROTEIN INTERACTIONS,TRANSCRIPTION FACTOR FAMILY,FLOWER DEVELOPMENT},
  language     = {eng},
  number       = {11},
  pages        = {2680--2693},
  title        = {Toward the analysis of the petunia MADS box gene family by reverse and forward transposon insertion mutagenesis approaches: B, C, and D floral organ identity functions require SEPALLATA-like MADS box genes in petunia},
  url          = {http://dx.doi.org/10.1105/tpc.017376},
  volume       = {15},
  year         = {2003},
}

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