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Different responses of tobacco antioxidant enzymes to light and chilling stress

(2003) JOURNAL OF PLANT PHYSIOLOGY. 160(5). p.509-515
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Abstract
The effect of elevated light treatment (25 degreesC, PPFD 360 mumol m(-2) sec(-1)) or chilling temperatures combined with elevated light (5 degreesC, PPFD 360 mumol m-2 sec-1) on the activity of six antioxidant enzymes, guaiacol peroxidases, and glutathione peroxidase (GPx, EC 1.11.1.9) protein accumulation were studied in tobacco Nicotiana tabacum cv. Petit Havana SR1. Both treatments caused no photo-oxidative damage, but chilling caused a transient wilting. The light treatment increased the activities of ascorbate peroxidase (APx, EC 1.11.1.11) and guaiacol peroxidases while catalase (EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and glutathione reductase (EC 1.6.4.2) were unchanged. In contrast, chilling treatment did not increase any of the antioxidant enzyme activities, but decreased catalase and to a lesser extent DHAR activities, Glutathione peroxidase protein levels increased sporadically under light treatment and constantly under chilling. Both chilling and light stress caused induction of glutathione synthesis and accumulation of oxidised glutathione, although the predominant part of the glutathione pool remained in the reduced form. Antioxidant enzymes from the chilling treated plants were measured at both 25 degreesC and 5 degreesC. Measurements at 5 degreesC revealed a 3-fold reduction in catalase activity, compared with that measured at 25degreesC, indicating that the overall reduction in catalase after four days of chilling was approximately 10-fold. The overall reduction in activity for the other antioxidant enzymes after four days of chilling was 2-fold for GR and APx, 1.5 fold for MDHAR, 3.5-fold for DHAR. The activity of SOD was the same at 25 and 5 degreesC. These results indicate that catalase and DHAR are most strongly affected by the chilling treatment and may be the rate-limiting factor of the antioxidant system at low temperatures.
Keywords
ENHANCED PHOTOOXIDATION, GLUTATHIONE-PEROXIDASE, ASCORBATE PEROXIDASE, MAIZE SEEDLINGS, OXIDATIVE STRESS, TRANSGENIC TOBACCO, SUPEROXIDE-DISMUTASE, light stress, glutathione peroxidase, antioxidant enzymes, chilling stress, ACTIVE OXYGEN, VISIBLE-LIGHT, PLANTS

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MLA
Gechev, Tsanko, Hilde Willekens, Marc Van Montagu, et al. “Different Responses of Tobacco Antioxidant Enzymes to Light and Chilling Stress.” JOURNAL OF PLANT PHYSIOLOGY 160.5 (2003): 509–515. Print.
APA
Gechev, Tsanko, Willekens, H., Van Montagu, M., Inzé, D., Van Camp, W., Toneva, V., & Minkov, I. (2003). Different responses of tobacco antioxidant enzymes to light and chilling stress. JOURNAL OF PLANT PHYSIOLOGY, 160(5), 509–515.
Chicago author-date
Gechev, Tsanko, Hilde Willekens, Marc Van Montagu, Dirk Inzé, Wim Van Camp, Valentina Toneva, and Ivan Minkov. 2003. “Different Responses of Tobacco Antioxidant Enzymes to Light and Chilling Stress.” Journal of Plant Physiology 160 (5): 509–515.
Chicago author-date (all authors)
Gechev, Tsanko, Hilde Willekens, Marc Van Montagu, Dirk Inzé, Wim Van Camp, Valentina Toneva, and Ivan Minkov. 2003. “Different Responses of Tobacco Antioxidant Enzymes to Light and Chilling Stress.” Journal of Plant Physiology 160 (5): 509–515.
Vancouver
1.
Gechev T, Willekens H, Van Montagu M, Inzé D, Van Camp W, Toneva V, et al. Different responses of tobacco antioxidant enzymes to light and chilling stress. JOURNAL OF PLANT PHYSIOLOGY. 2003;160(5):509–15.
IEEE
[1]
T. Gechev et al., “Different responses of tobacco antioxidant enzymes to light and chilling stress,” JOURNAL OF PLANT PHYSIOLOGY, vol. 160, no. 5, pp. 509–515, 2003.
@article{214692,
  abstract     = {The effect of elevated light treatment (25 degreesC, PPFD 360 mumol m(-2) sec(-1)) or chilling temperatures combined with elevated light (5 degreesC, PPFD 360 mumol m-2 sec-1) on the activity of six antioxidant enzymes, guaiacol peroxidases, and glutathione peroxidase (GPx, EC 1.11.1.9) protein accumulation were studied in tobacco Nicotiana tabacum cv. Petit Havana SR1. Both treatments caused no photo-oxidative damage, but chilling caused a transient wilting. The light treatment increased the activities of ascorbate peroxidase (APx, EC 1.11.1.11) and guaiacol peroxidases while catalase (EC 1.11.1.6), superoxide dismutase (SOD, EC 1.15.1.1), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and glutathione reductase (EC 1.6.4.2) were unchanged. In contrast, chilling treatment did not increase any of the antioxidant enzyme activities, but decreased catalase and to a lesser extent DHAR activities, Glutathione peroxidase protein levels increased sporadically under light treatment and constantly under chilling. Both chilling and light stress caused induction of glutathione synthesis and accumulation of oxidised glutathione, although the predominant part of the glutathione pool remained in the reduced form. Antioxidant enzymes from the chilling treated plants were measured at both 25 degreesC and 5 degreesC. Measurements at 5 degreesC revealed a 3-fold reduction in catalase activity, compared with that measured at 25degreesC, indicating that the overall reduction in catalase after four days of chilling was approximately 10-fold. The overall reduction in activity for the other antioxidant enzymes after four days of chilling was 2-fold for GR and APx, 1.5 fold for MDHAR, 3.5-fold for DHAR. The activity of SOD was the same at 25 and 5 degreesC. These results indicate that catalase and DHAR are most strongly affected by the chilling treatment and may be the rate-limiting factor of the antioxidant system at low temperatures.},
  author       = {Gechev, Tsanko and Willekens, Hilde and Van Montagu, Marc and Inzé, Dirk and Van Camp, Wim and Toneva, Valentina and Minkov, Ivan},
  issn         = {0176-1617},
  journal      = {JOURNAL OF PLANT PHYSIOLOGY},
  keywords     = {ENHANCED PHOTOOXIDATION,GLUTATHIONE-PEROXIDASE,ASCORBATE PEROXIDASE,MAIZE SEEDLINGS,OXIDATIVE STRESS,TRANSGENIC TOBACCO,SUPEROXIDE-DISMUTASE,light stress,glutathione peroxidase,antioxidant enzymes,chilling stress,ACTIVE OXYGEN,VISIBLE-LIGHT,PLANTS},
  language     = {eng},
  number       = {5},
  pages        = {509--515},
  title        = {Different responses of tobacco antioxidant enzymes to light and chilling stress},
  url          = {http://dx.doi.org/10.1078/0176-1617-00753},
  volume       = {160},
  year         = {2003},
}

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