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Screening for differential gene expression in Atropa belladonna leafy gall induced following Rhodococcus fascians infection

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Abstract
To better characterise at the molecular level the nature of plant responses to infection by Rhodococcus fascians PCR-based differential display patterns of Atropa belladonna leafy gall (LG) and non-infected plant tissues were compared. Six differentially expressed genes were identified and their altered expression was confirmed by RTPCR. Three of them corresponded to up-regulated genes which encode proteins involved in plant defence. The three remaining cDNA fragments which correspond to down-regulated genes in LG, encoded proteins with similarity to a multicystatin, a miraculin and a methallothionein-like protein, respectively. Upon elimination of the bacteria from infected plant tissue, the expression of up-regulated genes was maintained, whereas expression of down-regulated genes resumed suggesting a potential role of these up-regulated genes in plant growth and development.
Keywords
METALLOTHIONEIN-LIKE GENES, CYSTEINE PROTEINASE-INHIBITOR, TOBACCO, TOMATO, FRUIT, phytopathogenic bacteria, PR proteins, differential display

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Chicago
Nouar, El Hassan, Danny Vereecke, Koen Goethals, Mondher Jaziri, and Marie Baucher. 2003. “Screening for Differential Gene Expression in Atropa Belladonna Leafy Gall Induced Following Rhodococcus Fascians Infection.” European Journal of Plant Pathology 109 (4): 327–330.
APA
Nouar, E. H., Vereecke, D., Goethals, K., Jaziri, M., & Baucher, M. (2003). Screening for differential gene expression in Atropa belladonna leafy gall induced following Rhodococcus fascians infection. EUROPEAN JOURNAL OF PLANT PATHOLOGY, 109(4), 327–330.
Vancouver
1.
Nouar EH, Vereecke D, Goethals K, Jaziri M, Baucher M. Screening for differential gene expression in Atropa belladonna leafy gall induced following Rhodococcus fascians infection. EUROPEAN JOURNAL OF PLANT PATHOLOGY. 2003;109(4):327–30.
MLA
Nouar, El Hassan, Danny Vereecke, Koen Goethals, et al. “Screening for Differential Gene Expression in Atropa Belladonna Leafy Gall Induced Following Rhodococcus Fascians Infection.” EUROPEAN JOURNAL OF PLANT PATHOLOGY 109.4 (2003): 327–330. Print.
@article{214688,
  abstract     = {To better characterise at the molecular level the nature of plant responses to infection by Rhodococcus fascians PCR-based differential display patterns of Atropa belladonna leafy gall (LG) and non-infected plant tissues were compared. Six differentially expressed genes were identified and their altered expression was confirmed by RTPCR. Three of them corresponded to up-regulated genes which encode proteins involved in plant defence. The three remaining cDNA fragments which correspond to down-regulated genes in LG, encoded proteins with similarity to a multicystatin, a miraculin and a methallothionein-like protein, respectively. Upon elimination of the bacteria from infected plant tissue, the expression of up-regulated genes was maintained, whereas expression of down-regulated genes resumed suggesting a potential role of these up-regulated genes in plant growth and development.},
  author       = {Nouar, El Hassan and Vereecke, Danny and Goethals, Koen and Jaziri, Mondher and Baucher, Marie},
  issn         = {0929-1873},
  journal      = {EUROPEAN JOURNAL OF PLANT PATHOLOGY},
  keyword      = {METALLOTHIONEIN-LIKE GENES,CYSTEINE PROTEINASE-INHIBITOR,TOBACCO,TOMATO,FRUIT,phytopathogenic bacteria,PR proteins,differential display},
  language     = {eng},
  number       = {4},
  pages        = {327--330},
  title        = {Screening for differential gene expression in Atropa belladonna leafy gall induced following Rhodococcus fascians infection},
  url          = {http://dx.doi.org/10.1023/A:1023521626899},
  volume       = {109},
  year         = {2003},
}

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