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How does Trypanosoma equiperdum fit into the Trypanozoon group? : a cluster analysis by RAPD and multiplex-endonuclease genotyping approach

(2003) PARASITOLOGY. 126(5). p.425-431
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Abstract
The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and nagana. Previous molecular attempts to differentiate these species were not successful for T. evansi and T. equiperdum; only T. b. brucei could be differentiated to a certain extent. In this study we analysed 10 T. equiperdum, 8 T. evansi and 4 T. b. brucei using Random Amplified Polymorphic DNA (RAPD) and multiplex-endonuclease fingerprinting, a modified AFLP technique. The results obtained confirm the homogeneity of the T. evansi group tested. The T. b. brucei clustered out in a heterogenous group. For T. equiperdum the situation is more complex: 8 out of 10 T. equiperdum clustered together with the T. evansi group, while 2 T. equiperdum strains were more related to T. b. brucei. Hence, 2 hypotheses can be formulated: (1) only 2 T. equiperdum strains are genuine T. equiperdum causing dourine; all other T. equiperdum strains actually are T. evansi causing surra or (2) T. equiperdum does not exist at all. In that case, the different clinical outcome of horse infections with T. evansi or T. b. brucei is primarily related to the host immune response.
Keywords
Trypanosoma equiperdum, characterization, RAPD, multiplex-endonuclease fingerprinting, AFLP, KINETOPLAST DNA, EVANSI, EXPRESSION, IDENTIFICATION, STRAINS, PROBES, CHINA, GENES

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Chicago
Claes, F, EC Agbo, Magdalena Radwanska, MFW Te Pas, T Baltz, DT De Waal, Bruno Goddeeris, E Classen, and P Buscher. 2003. “How Does Trypanosoma Equiperdum Fit into the Trypanozoon Group? : a Cluster Analysis by RAPD and Multiplex-endonuclease Genotyping Approach.” Parasitology 126 (5): 425–431.
APA
Claes, F., Agbo, E., Radwanska, M., Te Pas, M., Baltz, T., De Waal, D., Goddeeris, B., et al. (2003). How does Trypanosoma equiperdum fit into the Trypanozoon group? : a cluster analysis by RAPD and multiplex-endonuclease genotyping approach. PARASITOLOGY, 126(5), 425–431.
Vancouver
1.
Claes F, Agbo E, Radwanska M, Te Pas M, Baltz T, De Waal D, et al. How does Trypanosoma equiperdum fit into the Trypanozoon group? : a cluster analysis by RAPD and multiplex-endonuclease genotyping approach. PARASITOLOGY. 2003;126(5):425–31.
MLA
Claes, F et al. “How Does Trypanosoma Equiperdum Fit into the Trypanozoon Group? : a Cluster Analysis by RAPD and Multiplex-endonuclease Genotyping Approach.” PARASITOLOGY 126.5 (2003): 425–431. Print.
@article{214380,
  abstract     = {The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and nagana. Previous molecular attempts to differentiate these species were not successful for T. evansi and T. equiperdum; only T. b. brucei could be differentiated to a certain extent. In this study we analysed 10 T. equiperdum, 8 T. evansi and 4 T. b. brucei using Random Amplified Polymorphic DNA (RAPD) and multiplex-endonuclease fingerprinting, a modified AFLP technique. The results obtained confirm the homogeneity of the T. evansi group tested. The T. b. brucei clustered out in a heterogenous group. For T. equiperdum the situation is more complex: 8 out of 10 T. equiperdum clustered together with the T. evansi group, while 2 T. equiperdum strains were more related to T. b. brucei. Hence, 2 hypotheses can be formulated: (1) only 2 T. equiperdum strains are genuine T. equiperdum causing dourine; all other T. equiperdum strains actually are T. evansi causing surra or (2) T. equiperdum does not exist at all. In that case, the different clinical outcome of horse infections with T. evansi or T. b. brucei is primarily related to the host immune response.},
  author       = {Claes, F and Agbo, EC and Radwanska, Magdalena and Te Pas, MFW and Baltz, T and De Waal, DT and Goddeeris, Bruno and Classen, E and Buscher, P},
  issn         = {0031-1820},
  journal      = {PARASITOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {425--431},
  title        = {How does Trypanosoma equiperdum fit into the Trypanozoon group? : a cluster analysis by RAPD and multiplex-endonuclease genotyping approach},
  url          = {http://dx.doi.org/10.1017/S0031182003002968},
  volume       = {126},
  year         = {2003},
}

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