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Qualitative and event-specific PCR real-time detection methods for StarLink maize

Pieter Windels, Sophie Bertrand UGent, Anna Depicker UGent, William Moens, Erik Van Bockstaele and Marc De Loose UGent (2003) EUROPEAN FOOD RESEARCH AND TECHNOLOGY. 216(3). p.259-263
abstract
In this paper we present qualitative detection and identification methods for StarLink maize (event CBH-351). The methodology proposed envisages detection of an internal target site in the cry9c coding region, as well as two event-specific target sites at the junction between the CBH-351 insert DNA and the genomic plant DNA. The cry9c-specific primer pair, generating a 180 bp amplicon, has been tested and optimised for conventional end-point PCR amplification. The event-specific primer pairs, generating amplicons of 138 bp and 100 bp respectively, give good performance in a conventional end-point PCR and in a real-time PCR assay. Our results clearly demonstrate that the primer pairs proposed can be used in an unambiguous and specific PCR identification assay for StarLink maize.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
identification, GMO, PCR, StarLink maize, junction region
journal title
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Eur. Food Res. Technol.
volume
216
issue
3
pages
259 - 263
Web of Science type
Article
Web of Science id
000182204500013
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
1.22 (2003)
JCR rank
17/94 (2003)
JCR quartile
1 (2003)
ISSN
1438-2377
DOI
10.1007/s00217-002-0652-5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
214326
handle
http://hdl.handle.net/1854/LU-214326
date created
2004-05-04 14:44:00
date last changed
2016-12-19 15:39:03
@article{214326,
  abstract     = {In this paper we present qualitative detection and identification methods for StarLink maize (event CBH-351). The methodology proposed envisages detection of an internal target site in the cry9c coding region, as well as two event-specific target sites at the junction between the CBH-351 insert DNA and the genomic plant DNA. The cry9c-specific primer pair, generating a 180 bp amplicon, has been tested and optimised for conventional end-point PCR amplification. The event-specific primer pairs, generating amplicons of 138 bp and 100 bp respectively, give good performance in a conventional end-point PCR and in a real-time PCR assay. Our results clearly demonstrate that the primer pairs proposed can be used in an unambiguous and specific PCR identification assay for StarLink maize.},
  author       = {Windels, Pieter and Bertrand, Sophie and Depicker, Anna and Moens, William and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {1438-2377},
  journal      = {EUROPEAN FOOD RESEARCH AND TECHNOLOGY},
  keyword      = {identification,GMO,PCR,StarLink maize,junction region},
  language     = {eng},
  number       = {3},
  pages        = {259--263},
  title        = {Qualitative and event-specific PCR real-time detection methods for StarLink maize},
  url          = {http://dx.doi.org/10.1007/s00217-002-0652-5},
  volume       = {216},
  year         = {2003},
}

Chicago
Windels, Pieter, Sophie Bertrand, Anna Depicker, William Moens, Erik Van Bockstaele, and Marc De Loose. 2003. “Qualitative and Event-specific PCR Real-time Detection Methods for StarLink Maize.” European Food Research and Technology 216 (3): 259–263.
APA
Windels, Pieter, Bertrand, S., Depicker, A., Moens, W., Van Bockstaele, E., & De Loose, M. (2003). Qualitative and event-specific PCR real-time detection methods for StarLink maize. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 216(3), 259–263.
Vancouver
1.
Windels P, Bertrand S, Depicker A, Moens W, Van Bockstaele E, De Loose M. Qualitative and event-specific PCR real-time detection methods for StarLink maize. EUROPEAN FOOD RESEARCH AND TECHNOLOGY. 2003;216(3):259–63.
MLA
Windels, Pieter, Sophie Bertrand, Anna Depicker, et al. “Qualitative and Event-specific PCR Real-time Detection Methods for StarLink Maize.” EUROPEAN FOOD RESEARCH AND TECHNOLOGY 216.3 (2003): 259–263. Print.