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Fermentation of animal components in strict carnivores: a comparitive study with cheetah fecal inoculum

Sarah Depauw, Guido Bosch, Myriam Hesta UGent, Katherine Whitehouse-Tedd, Wouter Hendriks, Jacques Kaandorp and Geert Janssens UGent (2012) JOURNAL OF ANIMAL SCIENCE. 90(8). p.2540-2548
abstract
The natural diet of felids contains highly digestible animal tissues but also fractions resistant to small intestinal digestion, which enter the large intestine where they may be fermented by the resident microbial population. Little information exists on the microbial degradability of animal tissues in the large intestine of felids consuming a natural diet. This study aimed to rank animal substrates in their microbial degradability by means of an in vitro study using captive cheetahs fed a strict carnivorous diet as fecal donors. Fresh cheetah fecal samples were collected, pooled, and incubated with various raw animal substrates (chicken cartilage, collagen, glucosamine-chondroitin, glucosamine, rabbit bone, rabbit hair, and rabbit skin; 4 replicates per substrate) for cumulative gas production measurement in a batch culture technique. Negative (cellulose) and positive (casein and fructo-oligosaccharides; FOS) controls were incorporated in the study. Additionally, after 72 h of incubation, short-chain fatty acids (SCFA), including branched-chain fatty acids (BCFA), and ammonia concentrations were determined for each substrate. Glucosamine and glucosamine-chondroitin yielded the greatest organic matter cumulative gas volume (OMCV) among animal substrates (P < 0.05), whereas total SCFA production was greatest for collagen (P < 0.05). Collagen induced an acetate production comparable with FOS and a markedly high acetate-to-propionate ratio (8.41: 1) compared with all other substrates (1.67: 1 to 2.97: 1). Chicken cartilage was rapidly fermentable, indicated by a greater maximal rate of gas production (R-max) compared with all other substrates (P < 0.05). In general, animal substrates showed an earlier occurrence for maximal gas production rate compared with FOS. Rabbit hair, skin, and bone were poorly fermentable substrates, indicated by the least amount of OMCV and total SCFA among animal substrates (P < 0.05). The greatest amount of ammonia production among animal substrates was measured after incubation of collagen and rabbit bone (P < 0.05). This study provides the first insight into the potential of animal tissues to influence large intestinal fermentation in a strict carnivore, and indicates that animal tissues have potentially similar functions as soluble or insoluble plant fibers in vitro. Further research is warranted to assess the impact of fermentation of each type of animal tissue on gastro-intestinal function and health in the cheetah and other felid species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
animal tissue, Acinonyx jubatus, felids, fermentation characteristics, in vitro, microbial degradation, IN-VITRO FERMENTATION, FATTY-ACID PRODUCTION, GAS-PRODUCTION, DIETARY FIBER, GASTROINTESTINAL-TRACT, LARGE-INTESTINE, BOVINE TENDON, CATS, METABOLISM, PROTEIN
journal title
JOURNAL OF ANIMAL SCIENCE
J. Anim. Sci.
volume
90
issue
8
pages
2540 - 2548
Web of Science type
Article
Web of Science id
000308040400012
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.093 (2012)
JCR rank
5/54 (2012)
JCR quartile
1 (2012)
ISSN
0021-8812
DOI
10.2527/jas.2011-4377
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2141687
handle
http://hdl.handle.net/1854/LU-2141687
date created
2012-06-13 11:06:49
date last changed
2016-12-19 15:42:06
@article{2141687,
  abstract     = {The natural diet of felids contains highly digestible animal tissues but also fractions resistant to small intestinal digestion, which enter the large intestine where they may be fermented by the resident microbial population. Little information exists on the microbial degradability of animal tissues in the large intestine of felids consuming a natural diet. This study aimed to rank animal substrates in their microbial degradability by means of an in vitro study using captive cheetahs fed a strict carnivorous diet as fecal donors. Fresh cheetah fecal samples were collected, pooled, and incubated with various raw animal substrates (chicken cartilage, collagen, glucosamine-chondroitin, glucosamine, rabbit bone, rabbit hair, and rabbit skin; 4 replicates per substrate) for cumulative gas production measurement in a batch culture technique. Negative (cellulose) and positive (casein and fructo-oligosaccharides; FOS) controls were incorporated in the study. Additionally, after 72 h of incubation, short-chain fatty acids (SCFA), including branched-chain fatty acids (BCFA), and ammonia concentrations were determined for each substrate. Glucosamine and glucosamine-chondroitin yielded the greatest organic matter cumulative gas volume (OMCV) among animal substrates (P {\textlangle} 0.05), whereas total SCFA production was greatest for collagen (P {\textlangle} 0.05). Collagen induced an acetate production comparable with FOS and a markedly high acetate-to-propionate ratio (8.41: 1) compared with all other substrates (1.67: 1 to 2.97: 1). Chicken cartilage was rapidly fermentable, indicated by a greater maximal rate of gas production (R-max) compared with all other substrates (P {\textlangle} 0.05). In general, animal substrates showed an earlier occurrence for maximal gas production rate compared with FOS. Rabbit hair, skin, and bone were poorly fermentable substrates, indicated by the least amount of OMCV and total SCFA among animal substrates (P {\textlangle} 0.05). The greatest amount of ammonia production among animal substrates was measured after incubation of collagen and rabbit bone (P {\textlangle} 0.05). This study provides the first insight into the potential of animal tissues to influence large intestinal fermentation in a strict carnivore, and indicates that animal tissues have potentially similar functions as soluble or insoluble plant fibers in vitro. Further research is warranted to assess the impact of fermentation of each type of animal tissue on gastro-intestinal function and health in the cheetah and other felid species.},
  author       = {Depauw, Sarah and Bosch, Guido and Hesta, Myriam and Whitehouse-Tedd, Katherine and Hendriks, Wouter and Kaandorp, Jacques and Janssens, Geert},
  issn         = {0021-8812},
  journal      = {JOURNAL OF ANIMAL SCIENCE},
  keyword      = {animal tissue,Acinonyx jubatus,felids,fermentation characteristics,in vitro,microbial degradation,IN-VITRO FERMENTATION,FATTY-ACID PRODUCTION,GAS-PRODUCTION,DIETARY FIBER,GASTROINTESTINAL-TRACT,LARGE-INTESTINE,BOVINE TENDON,CATS,METABOLISM,PROTEIN},
  language     = {eng},
  number       = {8},
  pages        = {2540--2548},
  title        = {Fermentation of animal components in strict carnivores: a comparitive study with cheetah fecal inoculum},
  url          = {http://dx.doi.org/10.2527/jas.2011-4377},
  volume       = {90},
  year         = {2012},
}

Chicago
Depauw, Sarah, Guido Bosch, Myriam Hesta, Katherine Whitehouse-Tedd, Wouter Hendriks, Jacques Kaandorp, and Geert Janssens. 2012. “Fermentation of Animal Components in Strict Carnivores: a Comparitive Study with Cheetah Fecal Inoculum.” Journal of Animal Science 90 (8): 2540–2548.
APA
Depauw, Sarah, Bosch, G., Hesta, M., Whitehouse-Tedd, K., Hendriks, W., Kaandorp, J., & Janssens, G. (2012). Fermentation of animal components in strict carnivores: a comparitive study with cheetah fecal inoculum. JOURNAL OF ANIMAL SCIENCE, 90(8), 2540–2548.
Vancouver
1.
Depauw S, Bosch G, Hesta M, Whitehouse-Tedd K, Hendriks W, Kaandorp J, et al. Fermentation of animal components in strict carnivores: a comparitive study with cheetah fecal inoculum. JOURNAL OF ANIMAL SCIENCE. 2012;90(8):2540–8.
MLA
Depauw, Sarah, Guido Bosch, Myriam Hesta, et al. “Fermentation of Animal Components in Strict Carnivores: a Comparitive Study with Cheetah Fecal Inoculum.” JOURNAL OF ANIMAL SCIENCE 90.8 (2012): 2540–2548. Print.