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Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women

Katrien Koppo UGent, Michaela Siklová-Vitková, Eva Klimčáková, Jan Polák, Marie A Marques, Michel Berlan, Johan Van de Voorde UGent, Jens Bulow, Dominique Langin and Isabelle de Glisezinski, et al. (2012) AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 302(2). p.E226-E232
abstract
The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the alpha(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FAT-CELLS, IN-VIVO, RESISTANCE, SENSITIVITY, EXPRESSION, EXERCISE, BODY, EPINEPHRINE, CALORIE RESTRICTION, HYPOCALORIC DIET, dietary intervention, microdialysis, glycerol, adrenaline, alpha(2)-adrenergic responsiveness
journal title
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Am. J. Physiol.-Endocrinol. Metab.
volume
302
issue
2
pages
E226 - E232
Web of Science type
Article
Web of Science id
000299112700008
JCR category
PHYSIOLOGY
JCR impact factor
4.514 (2012)
JCR rank
9/79 (2012)
JCR quartile
1 (2012)
ISSN
0193-1849
DOI
10.1152/ajpendo.00240.2011
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2138410
handle
http://hdl.handle.net/1854/LU-2138410
date created
2012-06-11 11:45:19
date last changed
2015-06-17 10:04:57
@article{2138410,
  abstract     = {The aim of this study was to investigate the evolution of the adrenergic and insulin-mediated regulation of lipolysis during different phases of a 6-mo dietary intervention. Eight obese women underwent a 6-mo dietary intervention consisting of a 1-mo very low-calorie diet (VLCD) followed by a 2-mo low-calorie diet (LCD) and 3-mo weight maintenance (WM) diet. At each phase of the dietary intervention, microdialysis of subcutaneous adipose tissue (SCAT) was performed at rest and during a 3-h hyperinsulinemic euglycemic clamp. Responses of dialysate glycerol concentration (DGC) were determined at baseline and during local perfusions with adrenaline or adrenaline and phentolamine before and during the last 30 min of the clamp. Dietary intervention induced a body weight reduction and an improved insulin sensitivity. DGC progressively decreased during the clamp, and this decrease was similar during the different phases of the diet. The adrenaline-induced increase in DGC was higher at VLCD and LCD compared with baseline condition and returned to prediet levels at WM. In the probe with adrenaline and phentolamine, the increase in DGC was higher than that in the adrenaline probe at baseline and WM, but it was not different at VLCD and LCD. The results suggest that the responsiveness of SCAT to adrenaline-stimulated lipolysis increases during the calorie-restricted phases due to a reduction of the alpha(2)-adrenoceptor-mediated antilipolytic action of adrenaline. At WM, adrenaline-stimulated lipolysis returned to the prediet levels. Furthermore, no direct relationship between insulin sensitivity and the diet-induced changes in the regulation of lipolysis was found.},
  author       = {Koppo, Katrien and Siklov{\'a}-Vitkov{\'a}, Michaela and Klim\v{c}{\'a}kov{\'a}, Eva and Pol{\'a}k, Jan and Marques, Marie A and Berlan, Michel and Van de Voorde, Johan and Bulow, Jens and Langin, Dominique and de Glisezinski, Isabelle and Stich, Vladimir},
  issn         = {0193-1849},
  journal      = {AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM},
  keyword      = {FAT-CELLS,IN-VIVO,RESISTANCE,SENSITIVITY,EXPRESSION,EXERCISE,BODY,EPINEPHRINE,CALORIE RESTRICTION,HYPOCALORIC DIET,dietary intervention,microdialysis,glycerol,adrenaline,alpha(2)-adrenergic responsiveness},
  language     = {eng},
  number       = {2},
  pages        = {E226--E232},
  title        = {Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women},
  url          = {http://dx.doi.org/10.1152/ajpendo.00240.2011},
  volume       = {302},
  year         = {2012},
}

Chicago
Koppo, Katrien, Michaela Siklová-Vitková, Eva Klimčáková, Jan Polák, Marie A Marques, Michel Berlan, Johan Van de Voorde, et al. 2012. “Catecholamine and Insulin Control of Lipolysis in Subcutaneous Adipose Tissue During Long-term Diet-induced Weight Loss in Obese Women.” American Journal of Physiology-endocrinology and Metabolism 302 (2): E226–E232.
APA
Koppo, K., Siklová-Vitková, M., Klimčáková, E., Polák, J., Marques, M. A., Berlan, M., Van de Voorde, J., et al. (2012). Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 302(2), E226–E232.
Vancouver
1.
Koppo K, Siklová-Vitková M, Klimčáková E, Polák J, Marques MA, Berlan M, et al. Catecholamine and insulin control of lipolysis in subcutaneous adipose tissue during long-term diet-induced weight loss in obese women. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 2012;302(2):E226–E232.
MLA
Koppo, Katrien, Michaela Siklová-Vitková, Eva Klimčáková, et al. “Catecholamine and Insulin Control of Lipolysis in Subcutaneous Adipose Tissue During Long-term Diet-induced Weight Loss in Obese Women.” AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 302.2 (2012): E226–E232. Print.