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Impact of corneal cross-linking on drug penetration in an ex vivo porcine eye model

Markus Tschopp, Johannes Stary, Beatrice E Frueh, Wolfgang Thormann, Julie De Smet UGent, Jan Van Bocxlaer UGent and Christoph Tappeiner (2012) CORNEA. 31(3). p.222-226
abstract
Purpose: To analyze the influence of corneal cross-linking (CXL) using ultraviolet-A and riboflavin on corneal drug penetration of topically applied drugs. Methods: In an ex vivo porcine eye model, eyes were randomly assigned to CXL or control treatment. Central corneal thickness and anterior chamber depth were measured with a Pentacam device. In the CXL group, eyes were treated with CXL using ultraviolet-A (370 nm) and riboflavin, whereas in the control group only riboflavin was applied without irradiation. Subsequently, 0.3% ofloxacin (n = 40 eyes) or 1% voriconazole (n = 40 eyes) eye drops were applied to the cornea every 5 minutes for 30 minutes. Aqueous humour samples were obtained performing an anterior chamber tap. The concentrations of ofloxacin and voriconazole were determined with high-pressure liquid chromatography. Groups were compared performing a Mann-Whitney test. Results: In the CXL group, the mean concentration of ofloxacin (13.33 +/- 4.67 mu g/mL) and voriconazole (52.70 +/- 8.76 mu g/mL) was significantly lower than in the untreated control group (ofloxacin: 18.51 +/- 6.08 mu g/mL, P = 0.005; voriconazole: 62.43 +/- 13.5 mu g/mL, P = 0.01). This corresponds to a reduction in permeability of 27.98% for ofloxacin and 15.59% for voriconazole. Central corneal thickness and anterior chamber depth were comparable in the CXL and control groups (P. 0.05, each). Conclusions: CXL reduces the corneal permeability of ofloxacin and voriconazole. This may be of clinical significance, for example, in keratitis treatment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cross-linking, AQUEOUS-HUMOR, corneal penetration, ofloxacin, voriconazole, PROGRESSIVE KERATOCONUS, RABBIT CORNEA, COLLAGEN, ULTRAVIOLET, OFLOXACIN, FLUOROQUINOLONES, RIBOFLAVIN/UVA, CIPROFLOXACIN, LEVOFLOXACIN
journal title
CORNEA
Cornea
volume
31
issue
3
pages
222 - 226
Web of Science type
Article
Web of Science id
000300455800003
JCR category
OPHTHALMOLOGY
JCR impact factor
1.746 (2012)
JCR rank
24/58 (2012)
JCR quartile
2 (2012)
ISSN
0277-3740
DOI
10.1097/ICO.0B013E31823E29D5
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2137694
handle
http://hdl.handle.net/1854/LU-2137694
date created
2012-06-08 16:21:42
date last changed
2012-06-26 10:30:07
@article{2137694,
  abstract     = {Purpose: To analyze the influence of corneal cross-linking (CXL) using ultraviolet-A and riboflavin on corneal drug penetration of topically applied drugs. 
Methods: In an ex vivo porcine eye model, eyes were randomly assigned to CXL or control treatment. Central corneal thickness and anterior chamber depth were measured with a Pentacam device. In the CXL group, eyes were treated with CXL using ultraviolet-A (370 nm) and riboflavin, whereas in the control group only riboflavin was applied without irradiation. Subsequently, 0.3\% ofloxacin (n = 40 eyes) or 1\% voriconazole (n = 40 eyes) eye drops were applied to the cornea every 5 minutes for 30 minutes. Aqueous humour samples were obtained performing an anterior chamber tap. The concentrations of ofloxacin and voriconazole were determined with high-pressure liquid chromatography. Groups were compared performing a Mann-Whitney test. 
Results: In the CXL group, the mean concentration of ofloxacin (13.33 +/- 4.67 mu g/mL) and voriconazole (52.70 +/- 8.76 mu g/mL) was significantly lower than in the untreated control group (ofloxacin: 18.51 +/- 6.08 mu g/mL, P = 0.005; voriconazole: 62.43 +/- 13.5 mu g/mL, P = 0.01). This corresponds to a reduction in permeability of 27.98\% for ofloxacin and 15.59\% for voriconazole. Central corneal thickness and anterior chamber depth were comparable in the CXL and control groups (P. 0.05, each). 
Conclusions: CXL reduces the corneal permeability of ofloxacin and voriconazole. This may be of clinical significance, for example, in keratitis treatment.},
  author       = {Tschopp, Markus and Stary, Johannes and Frueh, Beatrice E and Thormann, Wolfgang and De Smet, Julie and Van Bocxlaer, Jan and Tappeiner, Christoph},
  issn         = {0277-3740},
  journal      = {CORNEA},
  keyword      = {cross-linking,AQUEOUS-HUMOR,corneal penetration,ofloxacin,voriconazole,PROGRESSIVE KERATOCONUS,RABBIT CORNEA,COLLAGEN,ULTRAVIOLET,OFLOXACIN,FLUOROQUINOLONES,RIBOFLAVIN/UVA,CIPROFLOXACIN,LEVOFLOXACIN},
  language     = {eng},
  number       = {3},
  pages        = {222--226},
  title        = {Impact of corneal cross-linking on drug penetration in an ex vivo porcine eye model},
  url          = {http://dx.doi.org/10.1097/ICO.0B013E31823E29D5},
  volume       = {31},
  year         = {2012},
}

Chicago
Tschopp, Markus, Johannes Stary, Beatrice E Frueh, Wolfgang Thormann, Julie De Smet, Jan Van Bocxlaer, and Christoph Tappeiner. 2012. “Impact of Corneal Cross-linking on Drug Penetration in an Ex Vivo Porcine Eye Model.” Cornea 31 (3): 222–226.
APA
Tschopp, M., Stary, J., Frueh, B. E., Thormann, W., De Smet, J., Van Bocxlaer, J., & Tappeiner, C. (2012). Impact of corneal cross-linking on drug penetration in an ex vivo porcine eye model. CORNEA, 31(3), 222–226.
Vancouver
1.
Tschopp M, Stary J, Frueh BE, Thormann W, De Smet J, Van Bocxlaer J, et al. Impact of corneal cross-linking on drug penetration in an ex vivo porcine eye model. CORNEA. 2012;31(3):222–6.
MLA
Tschopp, Markus, Johannes Stary, Beatrice E Frueh, et al. “Impact of Corneal Cross-linking on Drug Penetration in an Ex Vivo Porcine Eye Model.” CORNEA 31.3 (2012): 222–226. Print.