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Cytotoxic effects of gold nanoparticles: a multiparametric study

Stefaan Soenen UGent, Bella Manshian, José María Montenegro, Faheem Amin, Björn Meermann UGent, Toke Thiron UGent, Maria Cornelissen UGent, Frank Vanhaecke UGent, Shareen Doak and Wolfgang J Parak, et al. (2012) ACS NANO. 6(7). p.5767-5783
abstract
The in vitro labeling of therapeutic cells with nanoparticles (NPs) is becoming more and more common, but concerns about the possible effects of the NPs on the cultured cells are also increasing. In the present work, we evaluate the effects of poly(methacrylic acid)-coated 4 nm diameter Au NPs on a variety of sensitive and therapeutically interesting cell types (C17.2 neural progenitor cells, human umbilical vein endothelial cells, and PC12 rat pheochromocytoma cells) using a multiparametric approach. Using various NP concentrations and incubation times, we performed a stepwise analysis of the NP effects on cell viability, reactive oxygen species, cell morphology, cytoskeleton architecture, and cell functionality. The data show that higher NP concentrations (209 nM) reduce cell viability mostly through induction of reactive oxygen species, which was significantly induced at concentrations of 50 nM Au NPs or higher. At these concentrations, both actin and tubulin cytoskeleton were deformed and resulted in reduced cell proliferation and cellular differentiation. In terms of cell functionality, the NPs significantly impeded neurite outgrowth of PC12 cells up to 20 nM concentrations. At 10 nM, no significant effects on any cellular parameter could be observed. These data highlight the importance of using multiple assays to cover the broad spectrum of cell-NP interactions and to determine safe NP concentrations and put forward the described protocol as a possible template for future cell-NP interaction studies under comparable and standardized conditions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
gold nanoparticles, nanotoxicology, cytotoxicity, biocompatibility, nanoparticle-cell interaction, FOCAL ADHESION KINASE, CELLULAR UPTAKE, IN-VIVO, INORGANIC NANOPARTICLES, OXIDATIVE STRESS, NANOTECHNOLOGY, NANOMEDICINE, TOXICITY, SIZE, NANORODS
journal title
ACS NANO
ACS Nano
volume
6
issue
7
pages
5767 - 5783
Web of Science type
Article
Web of Science id
000306673800005
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
12.062 (2012)
JCR rank
9/239 (2012)
JCR quartile
1 (2012)
ISSN
1936-0851
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2137388
handle
http://hdl.handle.net/1854/LU-2137388
date created
2012-06-08 13:51:55
date last changed
2014-05-26 09:44:56
@article{2137388,
  abstract     = {The in vitro labeling of therapeutic cells with nanoparticles (NPs) is becoming more and more common, but concerns about the possible effects of the NPs on the cultured cells are also increasing. In the present work, we evaluate the effects of poly(methacrylic acid)-coated 4 nm diameter Au NPs on a variety of sensitive and therapeutically interesting cell types (C17.2 neural progenitor cells, human umbilical vein endothelial cells, and PC12 rat pheochromocytoma cells) using a multiparametric approach. Using various NP concentrations and incubation times, we performed a stepwise analysis of the NP effects on cell viability, reactive oxygen species, cell morphology, cytoskeleton architecture, and cell functionality. The data show that higher NP concentrations (209 nM) reduce cell viability mostly through induction of reactive oxygen species, which was significantly induced at concentrations of 50 nM Au NPs or higher. At these concentrations, both actin and tubulin cytoskeleton were deformed and resulted in reduced cell proliferation and cellular differentiation. In terms of cell functionality, the NPs significantly impeded neurite outgrowth of PC12 cells up to 20 nM concentrations. At 10 nM, no significant effects on any cellular parameter could be observed. These data highlight the importance of using multiple assays to cover the broad spectrum of cell-NP interactions and to determine safe NP concentrations and put forward the described protocol as a possible template for future cell-NP interaction studies under comparable and standardized conditions.},
  author       = {Soenen, Stefaan and Manshian, Bella and Montenegro, Jos{\'e} Mar{\'i}a and Amin, Faheem and Meermann, Bj{\"o}rn and Thiron, Toke and Cornelissen, Maria and Vanhaecke, Frank and Doak, Shareen  and Parak, Wolfgang J and De Smedt, Stefaan and Braeckmans, Kevin},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  keyword      = {gold nanoparticles,nanotoxicology,cytotoxicity,biocompatibility,nanoparticle-cell interaction,FOCAL ADHESION KINASE,CELLULAR UPTAKE,IN-VIVO,INORGANIC NANOPARTICLES,OXIDATIVE STRESS,NANOTECHNOLOGY,NANOMEDICINE,TOXICITY,SIZE,NANORODS},
  language     = {eng},
  number       = {7},
  pages        = {5767--5783},
  title        = {Cytotoxic effects of gold nanoparticles: a multiparametric study},
  volume       = {6},
  year         = {2012},
}

Chicago
Soenen, Stefaan, Bella Manshian, José María Montenegro, Faheem Amin, Björn Meermann, Toke Thiron, Maria Cornelissen, et al. 2012. “Cytotoxic Effects of Gold Nanoparticles: a Multiparametric Study.” Acs Nano 6 (7): 5767–5783.
APA
Soenen, S., Manshian, B., Montenegro, J. M., Amin, F., Meermann, B., Thiron, T., Cornelissen, M., et al. (2012). Cytotoxic effects of gold nanoparticles: a multiparametric study. ACS NANO, 6(7), 5767–5783.
Vancouver
1.
Soenen S, Manshian B, Montenegro JM, Amin F, Meermann B, Thiron T, et al. Cytotoxic effects of gold nanoparticles: a multiparametric study. ACS NANO. 2012;6(7):5767–83.
MLA
Soenen, Stefaan, Bella Manshian, José María Montenegro, et al. “Cytotoxic Effects of Gold Nanoparticles: a Multiparametric Study.” ACS NANO 6.7 (2012): 5767–5783. Print.