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Detection of allergenic ingredients using real-time PCR: a case study on hazelnut (Corylus avellena) and soy (Glycine max)

Céline Platteau, Marc De Loose, Bruno De Meulenaer UGent and Isabel Taverniers (2011) JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 59(20). p.10803-10814
abstract
Compliance with the European allergen labeling legislation (Directive 2007/68/EC) is only possible when coupled with appropriate methods to detect allergens in food. The aim of the current study was to develop new real-time PCR assays for the detection of hazelnut and soy and evaluate these assays via comparison with commercially available kits. Although the new assays were not as sensitive as the commercial qualitative assays, they proved to be more specific. Moreover, the cross-reactivity study indicated contamination of some of the food products used with either hazelnut or soy, which presents a risk for the allergic consumer. The assays were able to quantify as few as 5-15 genome copies. This unit, used to express analytical results for allergen detection by means of PCR, needs to be converted to a unit expressing the amount of allergenic ingredient in order to be informative. This study emphasizes that the use of real-time PCR for allergen quantification is complicated by the lack of appropriate reference materials for allergens.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VALIDATION, PRODUCTS, ANGIOSPERMS, Glycine max, soy, Corylus avellana, hazelnut, PCR, food allergy, allergen detection, POLYMERASE-CHAIN-REACTION, SESAME, ASSAYS, TRACES, PEANUT, PROCESSED FOODS, NUCLEAR-DNA AMOUNTS
journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
J. Agric. Food Chem.
volume
59
issue
20
pages
10803 - 10814
Web of Science type
Article
Web of Science id
000295915000005
JCR category
AGRICULTURE, MULTIDISCIPLINARY
JCR impact factor
2.823 (2011)
JCR rank
3/57 (2011)
JCR quartile
1 (2011)
ISSN
0021-8561
DOI
10.1021/jf202110f
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2136283
handle
http://hdl.handle.net/1854/LU-2136283
date created
2012-06-07 15:07:30
date last changed
2012-06-22 16:00:36
@article{2136283,
  abstract     = {Compliance with the European allergen labeling legislation (Directive 2007/68/EC) is only possible when coupled with appropriate methods to detect allergens in food. The aim of the current study was to develop new real-time PCR assays for the detection of hazelnut and soy and evaluate these assays via comparison with commercially available kits. Although the new assays were not as sensitive as the commercial qualitative assays, they proved to be more specific. Moreover, the cross-reactivity study indicated contamination of some of the food products used with either hazelnut or soy, which presents a risk for the allergic consumer. The assays were able to quantify as few as 5-15 genome copies. This unit, used to express analytical results for allergen detection by means of PCR, needs to be converted to a unit expressing the amount of allergenic ingredient in order to be informative. This study emphasizes that the use of real-time PCR for allergen quantification is complicated by the lack of appropriate reference materials for allergens.},
  author       = {Platteau, C{\'e}line and De Loose, Marc and De Meulenaer, Bruno and Taverniers, Isabel},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {VALIDATION,PRODUCTS,ANGIOSPERMS,Glycine max,soy,Corylus avellana,hazelnut,PCR,food allergy,allergen detection,POLYMERASE-CHAIN-REACTION,SESAME,ASSAYS,TRACES,PEANUT,PROCESSED FOODS,NUCLEAR-DNA AMOUNTS},
  language     = {eng},
  number       = {20},
  pages        = {10803--10814},
  title        = {Detection of allergenic ingredients using real-time PCR: a case study on hazelnut (Corylus avellena) and soy (Glycine max)},
  url          = {http://dx.doi.org/10.1021/jf202110f},
  volume       = {59},
  year         = {2011},
}

Chicago
Platteau, Céline, Marc De Loose, Bruno De Meulenaer, and Isabel Taverniers. 2011. “Detection of Allergenic Ingredients Using Real-time PCR: a Case Study on Hazelnut (Corylus Avellena) and Soy (Glycine Max).” Journal of Agricultural and Food Chemistry 59 (20): 10803–10814.
APA
Platteau, C., De Loose, M., De Meulenaer, B., & Taverniers, I. (2011). Detection of allergenic ingredients using real-time PCR: a case study on hazelnut (Corylus avellena) and soy (Glycine max). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(20), 10803–10814.
Vancouver
1.
Platteau C, De Loose M, De Meulenaer B, Taverniers I. Detection of allergenic ingredients using real-time PCR: a case study on hazelnut (Corylus avellena) and soy (Glycine max). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2011;59(20):10803–14.
MLA
Platteau, Céline, Marc De Loose, Bruno De Meulenaer, et al. “Detection of Allergenic Ingredients Using Real-time PCR: a Case Study on Hazelnut (Corylus Avellena) and Soy (Glycine Max).” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.20 (2011): 10803–10814. Print.