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Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro

(2010) JOURNAL OF DAIRY SCIENCE. 93(11). p.5286-5299
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Abstract
Rumen biohydrogenation kinetics of C18:3n-3 from several chemically or technologically treated linseed products and docosahexaenoic acid (DHA; C22:6n-3) addition to linseed oil were evaluated in vitro. Linseed products evaluated were linseed oil, crushed linseed, formaldehyde treated crushed linseed, sodium hydroxide/formaldehyde treated crushed linseed, extruded whole linseed (2 processing variants), extruded crushed linseed (2 processing variants), micronized crushed linseed, commercially available extruded linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil. Each product was incubated with rumen liquid using equal amounts of supplemented C18:3n-3 and fermentable substrate (freeze-dried total mixed ration) for 0, 0.5, 1, 2, 4, 6, 12, and 24 h using a batch culture technique. Disappearance of C18:3n-3 was measured to estimate the fractional biohydrogenation rate and lag time according to an exponential model and to calculate effective biohydrogenation of C18:3n-3, assuming a fractional passage rate of 0.060/h. Treatments showed no differences in rumen fermentation parameters, including gas production rate and volatile fatty acid concentration. Technological pretreatment (crushing) followed by chemical treatment applied as formaldehyde of linseed resulted in effective protection of C18:3n-3 against biohydrogenation. Additional chemical pretreatment (sodium hydroxide) before applying formaldehyde treatment did not further improve the effectiveness of protection. Extrusion of whole linseed compared with extrusion of crushed linseed was effective in reducing C18:3n-3 biohydrogenation, whereas the processing variants were not different in C18:3n-3 biohydrogenation. Crushed linseed, micronized crushed linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil did not reduce C18:3n-3 biohydrogenation. Compared with the other treatments, docosahexaenoic acid addition to linseed oil resulted in a comparable trans11, cis15-C18:2 biohydrogenation but a lesser trans10+11-C18:1 biohydrogenation. This suggests that addition of DHA in combination with linseed oil was effective only in inhibiting the last step of biohydrogenation from trans10+11-C18:1 to C18:0.
Keywords
linseed, fatty acid, in vitro, biohydrogenation, UNSATURATED FATTY-ACIDS, LINOLEIC-ACID, DAIRY-COWS, MILK-FAT, RUMINAL BIOHYDROGENATION, EXTRUDED LINSEED, HOLSTEIN COWS, CANOLA SEED, RUMEN, FLAXSEED

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Citation

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Chicago
Sterck, A, R Hovenier, Bruno Vlaeminck, AM van Vuuren, WH hendriks, and J Dijkstra. 2010. “Effects of Chemically or Technologically Treated Linseed Products and Docosahexaenoic Acid Addition to Linseed Oil on Biohydrogenation of C18:3n-3 in Vitro.” Journal of Dairy Science 93 (11): 5286–5299.
APA
Sterck, A., Hovenier, R., Vlaeminck, B., van Vuuren, A., hendriks, W., & Dijkstra, J. (2010). Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro. JOURNAL OF DAIRY SCIENCE, 93(11), 5286–5299.
Vancouver
1.
Sterck A, Hovenier R, Vlaeminck B, van Vuuren A, hendriks W, Dijkstra J. Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro. JOURNAL OF DAIRY SCIENCE. 2010;93(11):5286–99.
MLA
Sterck, A, R Hovenier, Bruno Vlaeminck, et al. “Effects of Chemically or Technologically Treated Linseed Products and Docosahexaenoic Acid Addition to Linseed Oil on Biohydrogenation of C18:3n-3 in Vitro.” JOURNAL OF DAIRY SCIENCE 93.11 (2010): 5286–5299. Print.
@article{2134725,
  abstract     = {Rumen biohydrogenation kinetics of C18:3n-3 from several chemically or technologically treated linseed products and docosahexaenoic acid (DHA; C22:6n-3) addition to linseed oil were evaluated in vitro. Linseed products evaluated were linseed oil, crushed linseed, formaldehyde treated crushed linseed, sodium hydroxide/formaldehyde treated crushed linseed, extruded whole linseed (2 processing variants), extruded crushed linseed (2 processing variants), micronized crushed linseed, commercially available extruded linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil. Each product was incubated with rumen liquid using equal amounts of supplemented C18:3n-3 and fermentable substrate (freeze-dried total mixed ration) for 0, 0.5, 1, 2, 4, 6, 12, and 24 h using a batch culture technique. Disappearance of C18:3n-3 was measured to estimate the fractional biohydrogenation rate and lag time according to an exponential model and to calculate effective biohydrogenation of C18:3n-3, assuming a fractional passage rate of 0.060/h. Treatments showed no differences in rumen fermentation parameters, including gas production rate and volatile fatty acid concentration. Technological pretreatment (crushing) followed by chemical treatment applied as formaldehyde of linseed resulted in effective protection of C18:3n-3 against biohydrogenation. Additional chemical pretreatment (sodium hydroxide) before applying formaldehyde treatment did not further improve the effectiveness of protection. Extrusion of whole linseed compared with extrusion of crushed linseed was effective in reducing C18:3n-3 biohydrogenation, whereas the processing variants were not different in C18:3n-3 biohydrogenation. Crushed linseed, micronized crushed linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil did not reduce C18:3n-3 biohydrogenation. Compared with the other treatments, docosahexaenoic acid addition to linseed oil resulted in a comparable trans11, cis15-C18:2 biohydrogenation but a lesser trans10+11-C18:1 biohydrogenation. This suggests that addition of DHA in combination with linseed oil was effective only in inhibiting the last step of biohydrogenation from trans10+11-C18:1 to C18:0.},
  author       = {Sterck, A and Hovenier, R and Vlaeminck, Bruno and van Vuuren, AM and hendriks, WH and Dijkstra, J},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {linseed,fatty acid,in vitro,biohydrogenation,UNSATURATED FATTY-ACIDS,LINOLEIC-ACID,DAIRY-COWS,MILK-FAT,RUMINAL BIOHYDROGENATION,EXTRUDED LINSEED,HOLSTEIN COWS,CANOLA SEED,RUMEN,FLAXSEED},
  language     = {eng},
  number       = {11},
  pages        = {5286--5299},
  title        = {Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro},
  url          = {http://dx.doi.org/10.3168/jds.2010-3144},
  volume       = {93},
  year         = {2010},
}

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