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Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence

Yannick De Smet UGent, Paul Goetghebeur UGent, Stefan Wanke, Pieter Asselman UGent and Marie-Stéphanie Samain UGent (2012) PLANT ECOLOGY AND EVOLUTION. 145(1). p.73-87
abstract
Background - The genus Epimedium is well known for its ornamental representatives. However, species boundaries and evolutionary relationships within the genus remain uncertain due to several difficulties. First, potentially diagnostic characters are generally not described with enough detail, hampering linkage of specimens to recognized taxa. Second, previous molecular studies failed to gain resolution, especially within the Chinese distribution area of the genus. Nevertheless, growing scientific interest in the medicinal properties of Epimedium has prompted the need for reliable identification of species. Aims and methods - This study aims at: (1) assessing genetic diversity within Epimedium, using nuclear and chloroplast DNA sequences in combination with AFLP fingerprinting, (2) delivering a detailed description for one potentially diagnostic character, pubescence of leaflets. Key results - The DNA sequences and AFLP fingerprints resulted in an unresolved polytomy for the Chinese representatives of Epimedium. Furthermore, this study provided detailed scanning electron microscope images of four clearly distinguishable types of leaflet pubescence. Conclusions - As AFLP is considered capable of detecting rare genetic differences in groups with low sequence variation, we suggest the lack of resolution in the Chinese clade to represent a hard polytomy. This is interpreted as additional evidence for the hypothesis of a recent origin for these taxa. As this implies that several recognized species are still in the process of differentiation, these difficulties in resolving evolutionary relationships are linked to difficulties in species delimitation. Furthermore, the lack of details in some species descriptions has led to the description of new taxa based on small variations. To tackle these problems, we propose a change in view on Epimedium taxonomy. In this view, broader taxonomic entities are recognized, characterized by clearly defined characters. The four types of pubescence described in this study can serve as a first step towards this new interpretation of Epimedium taxonomy.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PALAEOVEGETATION, INFERENCE, MATK, ICARIIN, ITS, leaflet pubescence, SEM, PHYLOGENETIC ANALYSIS, TEMPERATE PLANTS, MORPHOLOGICAL DATA, SPECIES-DIVERSITY, EAST-ASIA, SEQUENCES, trnK-matK, AFLP, recent divergence, Epimedium
journal title
PLANT ECOLOGY AND EVOLUTION
Plant Ecol. Evol.
volume
145
issue
1
pages
73 - 87
Web of Science type
Article
Web of Science id
000303074000010
JCR category
PLANT SCIENCES
JCR impact factor
1.192 (2012)
JCR rank
104/193 (2012)
JCR quartile
3 (2012)
ISSN
2032-3913
DOI
10.5091/plecevo.2012.646
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2134701
handle
http://hdl.handle.net/1854/LU-2134701
date created
2012-06-06 13:54:38
date last changed
2012-06-12 10:01:18
@article{2134701,
  abstract     = {Background - The genus Epimedium is well known for its ornamental representatives. However, species boundaries and evolutionary relationships within the genus remain uncertain due to several difficulties. First, potentially diagnostic characters are generally not described with enough detail, hampering linkage of specimens to recognized taxa. Second, previous molecular studies failed to gain resolution, especially within the Chinese distribution area of the genus. Nevertheless, growing scientific interest in the medicinal properties of Epimedium has prompted the need for reliable identification of species. 
Aims and methods - This study aims at: (1) assessing genetic diversity within Epimedium, using nuclear and chloroplast DNA sequences in combination with AFLP fingerprinting, (2) delivering a detailed description for one potentially diagnostic character, pubescence of leaflets. 
Key results - The DNA sequences and AFLP fingerprints resulted in an unresolved polytomy for the Chinese representatives of Epimedium. Furthermore, this study provided detailed scanning electron microscope images of four clearly distinguishable types of leaflet pubescence. 
Conclusions - As AFLP is considered capable of detecting rare genetic differences in groups with low sequence variation, we suggest the lack of resolution in the Chinese clade to represent a hard polytomy. This is interpreted as additional evidence for the hypothesis of a recent origin for these taxa. As this implies that several recognized species are still in the process of differentiation, these difficulties in resolving evolutionary relationships are linked to difficulties in species delimitation. Furthermore, the lack of details in some species descriptions has led to the description of new taxa based on small variations. To tackle these problems, we propose a change in view on Epimedium taxonomy. In this view, broader taxonomic entities are recognized, characterized by clearly defined characters. The four types of pubescence described in this study can serve as a first step towards this new interpretation of Epimedium taxonomy.},
  author       = {De Smet, Yannick and Goetghebeur, Paul and Wanke, Stefan and Asselman, Pieter and Samain, Marie-St{\'e}phanie},
  issn         = {2032-3913},
  journal      = {PLANT ECOLOGY AND EVOLUTION},
  keyword      = {PALAEOVEGETATION,INFERENCE,MATK,ICARIIN,ITS,leaflet pubescence,SEM,PHYLOGENETIC ANALYSIS,TEMPERATE PLANTS,MORPHOLOGICAL DATA,SPECIES-DIVERSITY,EAST-ASIA,SEQUENCES,trnK-matK,AFLP,recent divergence,Epimedium},
  language     = {eng},
  number       = {1},
  pages        = {73--87},
  title        = {Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence},
  url          = {http://dx.doi.org/10.5091/plecevo.2012.646},
  volume       = {145},
  year         = {2012},
}

Chicago
De Smet, Yannick, Paul Goetghebeur, Stefan Wanke, Pieter Asselman, and Marie-Stéphanie Samain. 2012. “Additional Evidence for Recent Divergence of Chinese Epimedium (Berberidaceae) Derived from AFLP, Chloroplast and Nuclear Data Supplemented with Characterisation of Leaflet Pubescence.” Plant Ecology and Evolution 145 (1): 73–87.
APA
De Smet, Y., Goetghebeur, P., Wanke, S., Asselman, P., & Samain, M.-S. (2012). Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence. PLANT ECOLOGY AND EVOLUTION, 145(1), 73–87.
Vancouver
1.
De Smet Y, Goetghebeur P, Wanke S, Asselman P, Samain M-S. Additional evidence for recent divergence of Chinese Epimedium (Berberidaceae) derived from AFLP, chloroplast and nuclear data supplemented with characterisation of leaflet pubescence. PLANT ECOLOGY AND EVOLUTION. 2012;145(1):73–87.
MLA
De Smet, Yannick, Paul Goetghebeur, Stefan Wanke, et al. “Additional Evidence for Recent Divergence of Chinese Epimedium (Berberidaceae) Derived from AFLP, Chloroplast and Nuclear Data Supplemented with Characterisation of Leaflet Pubescence.” PLANT ECOLOGY AND EVOLUTION 145.1 (2012): 73–87. Print.