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Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines

Dries Vercauteren, Hendrik Deschout UGent, Katrien Remaut UGent, Johan FJ Engbersen, Arwyn T Jones, Jo Demeester UGent, Stefaan De Smedt UGent and Kevin Braeckmans UGent (2011) ACS NANO. 5(10). p.7874-7884
abstract
To gain a better understanding of intracellular processing of nanomedicines, we employed quantitative live-cell fluorescence colocalization microscopy to study endosomal trafficking of polyplexes in retinal pigment epithelium cells. A new, dynamic colocalization algorithm was developed, based on particle tracking and trajectory correlation, allowing for spatiotemporal characterization of internalized polyplexes in comparison with endosomal compartments labeled with EGFP constructs. This revealed early trafficking of the polyplexes specifically to Rab5- and flotillin-2-positive vesicles and subsequent delivery to Rab7 and LAMP1-labeled late endolysosomes where the major fraction of the polyplexes remains entrapped for days, suggesting the functional loss of these nanomedicines. Colocalization of polyplexes with the autophagy marker LC3 suggests for the first time that the process of xenophagy could play an important role in the persistent endosomal entrapment of nanomedicines.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
particle tracking, AUTOPHAGY, PLASMID DNA, DRUG, intracellular trafficking, nonviral ocular gene therapy, nanomedicines, colocalization, autophagy, GENE DELIVERY, FLUORESCENCE MICROSCOPY, SINGLE MOLECULES, LIVING CELLS, PARTICLE TRACKING, LYSOSOMES, TRANSPORT
journal title
ACS NANO
ACS Nano
volume
5
issue
10
pages
7874 - 7884
Web of Science type
Article
Web of Science id
000296208700035
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
10.774 (2011)
JCR rank
9/229 (2011)
JCR quartile
1 (2011)
ISSN
1936-0851
DOI
10.1021/nn2020858
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2134082
handle
http://hdl.handle.net/1854/LU-2134082
date created
2012-06-06 11:28:30
date last changed
2017-05-31 13:12:53
@article{2134082,
  abstract     = {To gain a better understanding of intracellular processing of nanomedicines, we employed quantitative live-cell fluorescence colocalization microscopy to study endosomal trafficking of polyplexes in retinal pigment epithelium cells. A new, dynamic colocalization algorithm was developed, based on particle tracking and trajectory correlation, allowing for spatiotemporal characterization of internalized polyplexes in comparison with endosomal compartments labeled with EGFP constructs. This revealed early trafficking of the polyplexes specifically to Rab5- and flotillin-2-positive vesicles and subsequent delivery to Rab7 and LAMP1-labeled late endolysosomes where the major fraction of the polyplexes remains entrapped for days, suggesting the functional loss of these nanomedicines. Colocalization of polyplexes with the autophagy marker LC3 suggests for the first time that the process of xenophagy could play an important role in the persistent endosomal entrapment of nanomedicines.},
  author       = {Vercauteren, Dries and Deschout, Hendrik and Remaut, Katrien and Engbersen, Johan FJ and Jones, Arwyn T and Demeester, Jo and De Smedt, Stefaan and Braeckmans, Kevin},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  keyword      = {particle tracking,AUTOPHAGY,PLASMID DNA,DRUG,intracellular trafficking,nonviral ocular gene therapy,nanomedicines,colocalization,autophagy,GENE DELIVERY,FLUORESCENCE MICROSCOPY,SINGLE MOLECULES,LIVING CELLS,PARTICLE TRACKING,LYSOSOMES,TRANSPORT},
  language     = {eng},
  number       = {10},
  pages        = {7874--7884},
  title        = {Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines},
  url          = {http://dx.doi.org/10.1021/nn2020858},
  volume       = {5},
  year         = {2011},
}

Chicago
Vercauteren, Dries, Hendrik Deschout, Katrien Remaut, Johan FJ Engbersen, Arwyn T Jones, Jo Demeester, Stefaan De Smedt, and Kevin Braeckmans. 2011. “Dynamic Colocalization Microscopy to Characterize Intracellular Trafficking of Nanomedicines.” Acs Nano 5 (10): 7874–7884.
APA
Vercauteren, Dries, Deschout, H., Remaut, K., Engbersen, J. F., Jones, A. T., Demeester, J., De Smedt, S., et al. (2011). Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines. ACS NANO, 5(10), 7874–7884.
Vancouver
1.
Vercauteren D, Deschout H, Remaut K, Engbersen JF, Jones AT, Demeester J, et al. Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines. ACS NANO. 2011;5(10):7874–84.
MLA
Vercauteren, Dries, Hendrik Deschout, Katrien Remaut, et al. “Dynamic Colocalization Microscopy to Characterize Intracellular Trafficking of Nanomedicines.” ACS NANO 5.10 (2011): 7874–7884. Print.