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Oocyte quality determines bovine embryo development after fertilisation with hydrogen peroxide-stressed spermatozoa

Mohammad Bozlur Rahman UGent, Leen Vandaele UGent, Tom Rijsselaere UGent, Mahdi Zhandi, Dominiek Maes UGent, Mohammed Shamsuddin and Ann Van Soom UGent (2012) REPRODUCTION FERTILITY AND DEVELOPMENT. 24(4). p.608-618
abstract
Exposure of gametes to specific stressors at sublethal levels can enhance the gametes' subsequent performance in processes such as cryopreservation. In the present study, bull spermatozoa were subjected to H2O2 for 4 h at 100-, 200- and 500-mu M levels; computer-assisted sperm analysis (CASA) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay were used for evaluation of subsequent sperm motility and DNA integrity, respectively. Exposure of spermatozoa to H2O2 did not affect sperm motility but DNA integrity was negatively affected by 500 mu M H2O2 compared with mock-exposed spermatozoa, whereas both motility and DNA integrity were affected compared with untreated spermatozoa. Nevertheless, insemination of oocytes with spermatozoa exposed to 200 mu M H2O2 increased fertilisation, cleavage and blastocyst rates (P < 0.05). Furthermore, the higher blastocyst yield after fertilisation of oocytes with spermatozoa exposed to 200 mu M H2O2 was related to oocyte diameter, with large-medium oocytes yielding higher blastocyst rates, while small-diameter oocytes consistently failed to develop into blastocysts. In conclusion, the results indicate that exposure of spermatozoa to 200 mu M H2O2 before sperm-oocyte interaction may enhance in vitro embryo production in cattle. However, this increased embryo production is largely dependent on the intrinsic quality of the oocytes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
QUANTITATIVE-ANALYSIS, SUPEROXIDE-DISMUTASE, SPERM MOTILITY, OXIDATIVE STRESS, LIPID-PEROXIDATION, BULL SPERMATOZOA, HIGH HYDROSTATIC-PRESSURE, OXYGEN SPECIES PRODUCTION, total cell number, oxidative stress, DNA-DAMAGE, VITRO PRODUCED EMBRYOS, DNA damage
journal title
REPRODUCTION FERTILITY AND DEVELOPMENT
Reprod. Fertil. Dev.
volume
24
issue
4
pages
608 - 618
Web of Science type
Article
Web of Science id
000303123800010
JCR category
ZOOLOGY
JCR impact factor
2.583 (2012)
JCR rank
18/149 (2012)
JCR quartile
1 (2012)
ISSN
1031-3613
DOI
10.1071/RD11237
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2132355
handle
http://hdl.handle.net/1854/LU-2132355
date created
2012-06-05 10:19:38
date last changed
2012-06-15 13:31:01
@article{2132355,
  abstract     = {Exposure of gametes to specific stressors at sublethal levels can enhance the gametes' subsequent performance in processes such as cryopreservation. In the present study, bull spermatozoa were subjected to H2O2 for 4 h at 100-, 200- and 500-mu M levels; computer-assisted sperm analysis (CASA) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay were used for evaluation of subsequent sperm motility and DNA integrity, respectively. Exposure of spermatozoa to H2O2 did not affect sperm motility but DNA integrity was negatively affected by 500 mu M H2O2 compared with mock-exposed spermatozoa, whereas both motility and DNA integrity were affected compared with untreated spermatozoa. Nevertheless, insemination of oocytes with spermatozoa exposed to 200 mu M H2O2 increased fertilisation, cleavage and blastocyst rates (P {\textlangle} 0.05). Furthermore, the higher blastocyst yield after fertilisation of oocytes with spermatozoa exposed to 200 mu M H2O2 was related to oocyte diameter, with large-medium oocytes yielding higher blastocyst rates, while small-diameter oocytes consistently failed to develop into blastocysts. In conclusion, the results indicate that exposure of spermatozoa to 200 mu M H2O2 before sperm-oocyte interaction may enhance in vitro embryo production in cattle. However, this increased embryo production is largely dependent on the intrinsic quality of the oocytes.},
  author       = {Rahman, Mohammad Bozlur and Vandaele, Leen and Rijsselaere, Tom and Zhandi, Mahdi and Maes, Dominiek and Shamsuddin, Mohammed and Van Soom, Ann},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {QUANTITATIVE-ANALYSIS,SUPEROXIDE-DISMUTASE,SPERM MOTILITY,OXIDATIVE STRESS,LIPID-PEROXIDATION,BULL SPERMATOZOA,HIGH HYDROSTATIC-PRESSURE,OXYGEN SPECIES PRODUCTION,total cell number,oxidative stress,DNA-DAMAGE,VITRO PRODUCED EMBRYOS,DNA damage},
  language     = {eng},
  number       = {4},
  pages        = {608--618},
  title        = {Oocyte quality determines bovine embryo development after fertilisation with hydrogen peroxide-stressed spermatozoa},
  url          = {http://dx.doi.org/10.1071/RD11237},
  volume       = {24},
  year         = {2012},
}

Chicago
Rahman, Mohammad Bozlur, Leen Vandaele, Tom Rijsselaere, Mahdi Zhandi, Dominiek Maes, Mohammed Shamsuddin, and Ann Van Soom. 2012. “Oocyte Quality Determines Bovine Embryo Development After Fertilisation with Hydrogen Peroxide-stressed Spermatozoa.” Reproduction Fertility and Development 24 (4): 608–618.
APA
Rahman, M. B., Vandaele, L., Rijsselaere, T., Zhandi, M., Maes, D., Shamsuddin, M., & Van Soom, A. (2012). Oocyte quality determines bovine embryo development after fertilisation with hydrogen peroxide-stressed spermatozoa. REPRODUCTION FERTILITY AND DEVELOPMENT, 24(4), 608–618.
Vancouver
1.
Rahman MB, Vandaele L, Rijsselaere T, Zhandi M, Maes D, Shamsuddin M, et al. Oocyte quality determines bovine embryo development after fertilisation with hydrogen peroxide-stressed spermatozoa. REPRODUCTION FERTILITY AND DEVELOPMENT. 2012;24(4):608–18.
MLA
Rahman, Mohammad Bozlur, Leen Vandaele, Tom Rijsselaere, et al. “Oocyte Quality Determines Bovine Embryo Development After Fertilisation with Hydrogen Peroxide-stressed Spermatozoa.” REPRODUCTION FERTILITY AND DEVELOPMENT 24.4 (2012): 608–618. Print.