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Encapsulation performance of layer-by-layer microcapsules for proteins

Marie-Luce De Temmerman (UGent) , Jo Demeester (UGent) , Filip De Vos (UGent) and Stefaan De Smedt (UGent)
(2011) BIOMACROMOLECULES. 12(4). p.1283-1289
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Abstract
This study reports on the encapsulation efficiency of proteins in dextran sulfate/poly-l-arginine-based microcapsules, fabricated via layer-by-layer assembly (LbL). For this purpose, radiolabeled proteins are entrapped in CaCO3 microparticles, followed by LbL coating of the CaCO3 cores and subsequent dissolving of the CaCO3 using EDTA. To allow to improve protein encapsulation in LbL microcapsules, we studied all steps in the preparation of the microcapsules where loss of protein load might occur. The encapsulation efficiency of proteins in LbL microcapsules turns out to be strongly dependent on both the charge and molecular weight of the protein as well as on the number of polyelectrolyte bilayers the microcapsules consist of.
Keywords
PERMEABILITY, COLLOIDAL PARTICLES, MACROMOLECULES, MICROPARTICLES, DENDRITIC CELLS, NANOCAPSULES, RELEASE, CROSS-PRESENTATION, POLYELECTROLYTE MICROCAPSULES, MULTILAYER CAPSULES

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Chicago
De Temmerman, Marie-Luce, Jo Demeester, Filip De Vos, and Stefaan De Smedt. 2011. “Encapsulation Performance of Layer-by-layer Microcapsules for Proteins.” Biomacromolecules 12 (4): 1283–1289.
APA
De Temmerman, M.-L., Demeester, J., De Vos, F., & De Smedt, S. (2011). Encapsulation performance of layer-by-layer microcapsules for proteins. BIOMACROMOLECULES, 12(4), 1283–1289.
Vancouver
1.
De Temmerman M-L, Demeester J, De Vos F, De Smedt S. Encapsulation performance of layer-by-layer microcapsules for proteins. BIOMACROMOLECULES. 2011;12(4):1283–9.
MLA
De Temmerman, Marie-Luce, Jo Demeester, Filip De Vos, et al. “Encapsulation Performance of Layer-by-layer Microcapsules for Proteins.” BIOMACROMOLECULES 12.4 (2011): 1283–1289. Print.
@article{2131349,
  abstract     = {This study reports on the encapsulation efficiency of proteins in dextran sulfate/poly-l-arginine-based microcapsules, fabricated via layer-by-layer assembly (LbL). For this purpose, radiolabeled proteins are entrapped in CaCO3 microparticles, followed by LbL coating of the CaCO3 cores and subsequent dissolving of the CaCO3 using EDTA. To allow to improve protein encapsulation in LbL microcapsules, we studied all steps in the preparation of the microcapsules where loss of protein load might occur. The encapsulation efficiency of proteins in LbL microcapsules turns out to be strongly dependent on both the charge and molecular weight of the protein as well as on the number of polyelectrolyte bilayers the microcapsules consist of.},
  author       = {De Temmerman, Marie-Luce and Demeester, Jo and De Vos, Filip and De Smedt, Stefaan},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  language     = {eng},
  number       = {4},
  pages        = {1283--1289},
  title        = {Encapsulation performance of layer-by-layer microcapsules for proteins},
  url          = {http://dx.doi.org/10.1021/bm101559w},
  volume       = {12},
  year         = {2011},
}

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