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Encapsulation performance of layer-by-layer microcapsules for proteins

Marie-Luce De Temmerman UGent, Jo Demeester UGent, Filip De Vos UGent and Stefaan De Smedt UGent (2011) BIOMACROMOLECULES. 12(4). p.1283-1289
abstract
This study reports on the encapsulation efficiency of proteins in dextran sulfate/poly-l-arginine-based microcapsules, fabricated via layer-by-layer assembly (LbL). For this purpose, radiolabeled proteins are entrapped in CaCO3 microparticles, followed by LbL coating of the CaCO3 cores and subsequent dissolving of the CaCO3 using EDTA. To allow to improve protein encapsulation in LbL microcapsules, we studied all steps in the preparation of the microcapsules where loss of protein load might occur. The encapsulation efficiency of proteins in LbL microcapsules turns out to be strongly dependent on both the charge and molecular weight of the protein as well as on the number of polyelectrolyte bilayers the microcapsules consist of.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PERMEABILITY, COLLOIDAL PARTICLES, MACROMOLECULES, MICROPARTICLES, DENDRITIC CELLS, NANOCAPSULES, RELEASE, CROSS-PRESENTATION, POLYELECTROLYTE MICROCAPSULES, MULTILAYER CAPSULES
journal title
BIOMACROMOLECULES
Biomacromolecules
volume
12
issue
4
pages
1283 - 1289
Web of Science type
Article
Web of Science id
000289223500049
JCR category
POLYMER SCIENCE
JCR impact factor
5.479 (2011)
JCR rank
3/78 (2011)
JCR quartile
1 (2011)
ISSN
1525-7797
DOI
10.1021/bm101559w
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2131349
handle
http://hdl.handle.net/1854/LU-2131349
date created
2012-06-04 15:06:49
date last changed
2012-09-07 10:50:25
@article{2131349,
  abstract     = {This study reports on the encapsulation efficiency of proteins in dextran sulfate/poly-l-arginine-based microcapsules, fabricated via layer-by-layer assembly (LbL). For this purpose, radiolabeled proteins are entrapped in CaCO3 microparticles, followed by LbL coating of the CaCO3 cores and subsequent dissolving of the CaCO3 using EDTA. To allow to improve protein encapsulation in LbL microcapsules, we studied all steps in the preparation of the microcapsules where loss of protein load might occur. The encapsulation efficiency of proteins in LbL microcapsules turns out to be strongly dependent on both the charge and molecular weight of the protein as well as on the number of polyelectrolyte bilayers the microcapsules consist of.},
  author       = {De Temmerman, Marie-Luce and Demeester, Jo and De Vos, Filip and De Smedt, Stefaan},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {PERMEABILITY,COLLOIDAL PARTICLES,MACROMOLECULES,MICROPARTICLES,DENDRITIC CELLS,NANOCAPSULES,RELEASE,CROSS-PRESENTATION,POLYELECTROLYTE MICROCAPSULES,MULTILAYER CAPSULES},
  language     = {eng},
  number       = {4},
  pages        = {1283--1289},
  title        = {Encapsulation performance of layer-by-layer microcapsules for proteins},
  url          = {http://dx.doi.org/10.1021/bm101559w},
  volume       = {12},
  year         = {2011},
}

Chicago
De Temmerman, Marie-Luce, Jo Demeester, Filip De Vos, and Stefaan De Smedt. 2011. “Encapsulation Performance of Layer-by-layer Microcapsules for Proteins.” Biomacromolecules 12 (4): 1283–1289.
APA
De Temmerman, M.-L., Demeester, J., De Vos, F., & De Smedt, S. (2011). Encapsulation performance of layer-by-layer microcapsules for proteins. BIOMACROMOLECULES, 12(4), 1283–1289.
Vancouver
1.
De Temmerman M-L, Demeester J, De Vos F, De Smedt S. Encapsulation performance of layer-by-layer microcapsules for proteins. BIOMACROMOLECULES. 2011;12(4):1283–9.
MLA
De Temmerman, Marie-Luce, Jo Demeester, Filip De Vos, et al. “Encapsulation Performance of Layer-by-layer Microcapsules for Proteins.” BIOMACROMOLECULES 12.4 (2011): 1283–1289. Print.