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Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity

Lieven Van Waeyenberghe UGent, Frank Pasmans UGent, Katharina D'Herde UGent, Richard Ducatelle UGent, Herman Favoreel UGent, Shaoji Li UGent, Freddy Haesebrouck UGent and An Martel UGent (2012) VETERINARY RESEARCH. 43.
abstract
Although aspergillosis is one of the most common diseases in captive birds, the pathogenesis of avian aspergillosis is poorly known. We studied the role of avian respiratory macrophages as a first line of defense against avian aspergillosis. The phagocytic and killing capacities of avian respiratory macrophages were evaluated using pigeon respiratory macrophages that were inoculated with Aspergillus fumigatus conidia. On average, 25% of macrophage-associated conidia were phagocytosed after one hour. Sixteen percents of these cell-associated conidia were killed after 4 h and conidial germination was inhibited in more than 95% of the conidia. A. fumigatus conidia were shown to be cytotoxic to the macrophages. Intracellularly germinating conidia were located free in the cytoplasm of necrotic cells, as shown using transmission electron microscopy. These results suggest that avian respiratory macrophages may prevent early establishment of infection, unless the number of A. fumigatus conidia exceeds the macrophage killing capacity, leading to intracellular germination and colonization of the respiratory tract.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
VETERINARY RESEARCH
Vet. Res.
volume
43
article_number
32
pages
5 pages
Web of Science type
Article
Web of Science id
000307292000001
JCR category
VETERINARY SCIENCES
JCR impact factor
3.426 (2012)
JCR rank
1/142 (2012)
JCR quartile
1 (2012)
ISSN
0928-4249
DOI
10.1186/1297-9716-43-32
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2131311
handle
http://hdl.handle.net/1854/LU-2131311
date created
2012-06-04 14:51:58
date last changed
2012-09-11 11:32:15
@article{2131311,
  abstract     = {Although aspergillosis is one of the most common diseases in captive birds, the pathogenesis of avian aspergillosis is poorly known. We studied the role of avian respiratory macrophages as a first line of defense against avian aspergillosis. The phagocytic and killing capacities of avian respiratory macrophages were evaluated using pigeon respiratory macrophages that were inoculated with Aspergillus fumigatus conidia. On average, 25\% of macrophage-associated conidia were phagocytosed after one hour. Sixteen percents of these cell-associated conidia were killed after 4 h and conidial germination was inhibited in more than 95\% of the conidia. A. fumigatus conidia were shown to be cytotoxic to the macrophages. Intracellularly germinating conidia were located free in the cytoplasm of necrotic cells, as shown using transmission electron microscopy. These results suggest that avian respiratory macrophages may prevent early establishment of infection, unless the number of A. fumigatus conidia exceeds the macrophage killing capacity, leading to intracellular germination and colonization of the respiratory tract.},
  articleno    = {32},
  author       = {Van Waeyenberghe, Lieven and Pasmans, Frank and D'Herde, Katharina and Ducatelle, Richard and Favoreel, Herman and Li, Shaoji and Haesebrouck, Freddy and Martel, An},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {5},
  title        = {Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-32},
  volume       = {43},
  year         = {2012},
}

Chicago
Van Waeyenberghe, Lieven, Frank Pasmans, Katharina D’Herde, Richard Ducatelle, Herman Favoreel, Shaoji Li, Freddy Haesebrouck, and An Martel. 2012. “Germination of Aspergillus Fumigatus Inside Avian Respiratory Macrophages Is Associated with Cytotoxicity.” Veterinary Research 43.
APA
Van Waeyenberghe, L., Pasmans, F., D’Herde, K., Ducatelle, R., Favoreel, H., Li, S., Haesebrouck, F., et al. (2012). Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity. VETERINARY RESEARCH, 43.
Vancouver
1.
Van Waeyenberghe L, Pasmans F, D’Herde K, Ducatelle R, Favoreel H, Li S, et al. Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity. VETERINARY RESEARCH. 2012;43.
MLA
Van Waeyenberghe, Lieven, Frank Pasmans, Katharina D’Herde, et al. “Germination of Aspergillus Fumigatus Inside Avian Respiratory Macrophages Is Associated with Cytotoxicity.” VETERINARY RESEARCH 43 (2012): n. pag. Print.