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Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity

Lieven Van Waeyenberghe (UGent) , Frank Pasmans (UGent) , Katharina D'Herde (UGent) , Richard Ducatelle (UGent) , Herman Favoreel (UGent) , Shaoji Li (UGent) , Freddy Haesebrouck (UGent) and An Martel (UGent)
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Abstract
Although aspergillosis is one of the most common diseases in captive birds, the pathogenesis of avian aspergillosis is poorly known. We studied the role of avian respiratory macrophages as a first line of defense against avian aspergillosis. The phagocytic and killing capacities of avian respiratory macrophages were evaluated using pigeon respiratory macrophages that were inoculated with Aspergillus fumigatus conidia. On average, 25% of macrophage-associated conidia were phagocytosed after one hour. Sixteen percents of these cell-associated conidia were killed after 4 h and conidial germination was inhibited in more than 95% of the conidia. A. fumigatus conidia were shown to be cytotoxic to the macrophages. Intracellularly germinating conidia were located free in the cytoplasm of necrotic cells, as shown using transmission electron microscopy. These results suggest that avian respiratory macrophages may prevent early establishment of infection, unless the number of A. fumigatus conidia exceeds the macrophage killing capacity, leading to intracellular germination and colonization of the respiratory tract.

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Chicago
Van Waeyenberghe, Lieven, Frank Pasmans, Katharina D’Herde, Richard Ducatelle, Herman Favoreel, Shaoji Li, Freddy Haesebrouck, and An Martel. 2012. “Germination of Aspergillus Fumigatus Inside Avian Respiratory Macrophages Is Associated with Cytotoxicity.” Veterinary Research 43.
APA
Van Waeyenberghe, L., Pasmans, F., D’Herde, K., Ducatelle, R., Favoreel, H., Li, S., Haesebrouck, F., et al. (2012). Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity. VETERINARY RESEARCH, 43.
Vancouver
1.
Van Waeyenberghe L, Pasmans F, D’Herde K, Ducatelle R, Favoreel H, Li S, et al. Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity. VETERINARY RESEARCH. 2012;43.
MLA
Van Waeyenberghe, Lieven, Frank Pasmans, Katharina D’Herde, et al. “Germination of Aspergillus Fumigatus Inside Avian Respiratory Macrophages Is Associated with Cytotoxicity.” VETERINARY RESEARCH 43 (2012): n. pag. Print.
@article{2131311,
  abstract     = {Although aspergillosis is one of the most common diseases in captive birds, the pathogenesis of avian aspergillosis is poorly known. We studied the role of avian respiratory macrophages as a first line of defense against avian aspergillosis. The phagocytic and killing capacities of avian respiratory macrophages were evaluated using pigeon respiratory macrophages that were inoculated with Aspergillus fumigatus conidia. On average, 25\% of macrophage-associated conidia were phagocytosed after one hour. Sixteen percents of these cell-associated conidia were killed after 4 h and conidial germination was inhibited in more than 95\% of the conidia. A. fumigatus conidia were shown to be cytotoxic to the macrophages. Intracellularly germinating conidia were located free in the cytoplasm of necrotic cells, as shown using transmission electron microscopy. These results suggest that avian respiratory macrophages may prevent early establishment of infection, unless the number of A. fumigatus conidia exceeds the macrophage killing capacity, leading to intracellular germination and colonization of the respiratory tract.},
  articleno    = {32},
  author       = {Van Waeyenberghe, Lieven and Pasmans, Frank and D'Herde, Katharina and Ducatelle, Richard and Favoreel, Herman and Li, Shaoji and Haesebrouck, Freddy and Martel, An},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {5},
  title        = {Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity},
  url          = {http://dx.doi.org/10.1186/1297-9716-43-32},
  volume       = {43},
  year         = {2012},
}

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