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Bioactive porcine matrices in heart valve tissue engineering

Pamela Somers UGent, Filip De Somer UGent, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2012) JOURNAL OF HEART VALVE DISEASE. 21(4). p.535-543
abstract
Background and aim of the study: Platelet gel (PG), a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation. The study aim was to load acellular porcine aortic valve matrices with the PG-rich growth factors and to evaluate the effect on MSC repopulation. Methods: Ovine mesenchymal stem cells (oMSCs) were isolated from sheep bone marrow. Acellular porcine heart valve matrices (n = 3) were preloaded with heparin and incubated with the PG for 2 h. A quantitative sandwich enzyme immunoassay was used to examine the release of basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) from the matrices. oMSC repopulation was stimulated by static and dynamic culture. Results: The immunoassays revealed that heparin-preloaded PG-incubated matrices showed a sustained release of 56.28 pg/ml bFGF and 30.66 ng/ml TGF-beta 1 after 24 h. Dynamic culture induced oMSC invasion in growth factor-loaded matrices. Cell density results showed that dynamic culture significantly enhanced the repopulation of growth factor-loaded matrices (75 +/- 21 cells/mm(2)) when compared to static culture (26 +/- 10 cells/mm(2)). Conclusion: The incubation of a porcine aortic valve matrix with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction between cells and the extracellular matrix in order to optimize cellular repopulation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
FIBROBLAST-GROWTH-FACTOR, PLATELET-RICH PLASMA, MESENCHYMAL STEM-CELLS, CLINICAL-APPLICATIONS, SUSTAINED-RELEASE, FACTOR DELIVERY, HEPARIN, PROLIFERATION, SCAFFOLDS, DIFFERENTIATION
journal title
JOURNAL OF HEART VALVE DISEASE
J. Heart Valve Dis.
volume
21
issue
4
pages
535 - 543
conference name
6th Biennial meeting of the Society for Heart Valve Disease
conference location
Barcelona, Spain
conference start
2011-06-25
conference end
2011-06-28
Web of Science type
Article; Proceedings Paper
Web of Science id
000308573900021
JCR category
CARDIAC & CARDIOVASCULAR SYSTEMS
JCR impact factor
1.071 (2012)
JCR rank
96/120 (2012)
JCR quartile
4 (2012)
ISSN
0966-8519
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2129265
handle
http://hdl.handle.net/1854/LU-2129265
date created
2012-06-01 15:08:10
date last changed
2013-02-27 16:57:28
@article{2129265,
  abstract     = {Background and aim of the study: Platelet gel (PG), a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation. The study aim was to load acellular porcine aortic valve matrices with the PG-rich growth factors and to evaluate the effect on MSC repopulation. 
Methods: Ovine mesenchymal stem cells (oMSCs) were isolated from sheep bone marrow. Acellular porcine heart valve matrices (n = 3) were preloaded with heparin and incubated with the PG for 2 h. A quantitative sandwich enzyme immunoassay was used to examine the release of basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) from the matrices. oMSC repopulation was stimulated by static and dynamic culture. 
Results: The immunoassays revealed that heparin-preloaded PG-incubated matrices showed a sustained release of 56.28 pg/ml bFGF and 30.66 ng/ml TGF-beta 1 after 24 h. Dynamic culture induced oMSC invasion in growth factor-loaded matrices. Cell density results showed that dynamic culture significantly enhanced the repopulation of growth factor-loaded matrices (75 +/- 21 cells/mm(2)) when compared to static culture (26 +/- 10 cells/mm(2)). 
Conclusion: The incubation of a porcine aortic valve matrix with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction between cells and the extracellular matrix in order to optimize cellular repopulation.},
  author       = {Somers, Pamela and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  issn         = {0966-8519},
  journal      = {JOURNAL OF HEART VALVE DISEASE},
  keyword      = {FIBROBLAST-GROWTH-FACTOR,PLATELET-RICH PLASMA,MESENCHYMAL STEM-CELLS,CLINICAL-APPLICATIONS,SUSTAINED-RELEASE,FACTOR DELIVERY,HEPARIN,PROLIFERATION,SCAFFOLDS,DIFFERENTIATION},
  language     = {eng},
  location     = {Barcelona, Spain},
  number       = {4},
  pages        = {535--543},
  title        = {Bioactive porcine matrices in heart valve tissue engineering},
  volume       = {21},
  year         = {2012},
}

Chicago
Somers, Pamela, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Bioactive Porcine Matrices in Heart Valve Tissue Engineering.” Journal of Heart Valve Disease 21 (4): 535–543.
APA
Somers, P., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). Bioactive porcine matrices in heart valve tissue engineering. JOURNAL OF HEART VALVE DISEASE, 21(4), 535–543. Presented at the 6th Biennial meeting of the Society for Heart Valve Disease.
Vancouver
1.
Somers P, De Somer F, Cornelissen M, Thierens H, Van Nooten G. Bioactive porcine matrices in heart valve tissue engineering. JOURNAL OF HEART VALVE DISEASE. 2012;21(4):535–43.
MLA
Somers, Pamela, Filip De Somer, Maria Cornelissen, et al. “Bioactive Porcine Matrices in Heart Valve Tissue Engineering.” JOURNAL OF HEART VALVE DISEASE 21.4 (2012): 535–543. Print.