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Platelet gel supernatant as a potential tool to repopulate acellular heart valves

(2012) CELL PROLIFERATION. 45(4). p.378-385
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Abstract
Objective : The aim of this study was to repopulate decellularized heart valve matrices with ovine mesenchymal stem cells (oMSCs) by the use of platelet gel (PG) supernatant, a storage vehicle for growth factors. Methods : oMSCs were exposed to different concentrations of PG-released supernatant and cell proliferation was evaluated using the MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. A quantitative sandwich enzyme immunoassay was used to examine amounts of bFGF and TGF-beta 1 in the PG supernatant. Repopulation of acellular heart valve matrices was stimulated by seeding matrices with oMSCs supplemented with the PG supernatant. Results : The most significant increase in proliferation induced by PG supernatant appeared at 1x105plts/ml concentration. Higher concentrations evoked reduction of the stimulatory process. oMSC motility was most significantly stimulated at 1x106plts/ml. Stimulating invasiveness of oMSCs needed the much higher concentration of 2x106plts/ml. Immunoassays revealed that sheep PG supernatant contains 184.8pg/ml bFGF and 60.5ng/ml TGF-beta 1. Moreover, repopulation of acellular heart valve matrices was significantly enhanced by PG supernatant addition and resulted in upregulation of the myofibroblast marker alpha-smooth muscle actin. Conclusions : Growth factors released from platelets had the potential to induce cell repopulation in a heart valve tissue engineering procedure, through stimulation of mesenchymal stem-cell migration and invasion.
Keywords
HUMAN ENDOTHELIAL-CELLS, MESENCHYMAL STEM-CELLS, GROWTH-FACTOR LEVELS, RICH PLASMA, CLINICAL-APPLICATIONS, PROLIFERATION, DIFFERENTIATION, IDENTIFICATION, BLOOD

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MLA
Somers, Pamela, Lieselot Robyns, Evelien Nollet, et al. “Platelet Gel Supernatant as a Potential Tool to Repopulate Acellular Heart Valves.” CELL PROLIFERATION 45.4 (2012): 378–385. Print.
APA
Somers, P., Robyns, L., Nollet, E., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). Platelet gel supernatant as a potential tool to repopulate acellular heart valves. CELL PROLIFERATION, 45(4), 378–385.
Chicago author-date
Somers, Pamela, Lieselot Robyns, Evelien Nollet, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Platelet Gel Supernatant as a Potential Tool to Repopulate Acellular Heart Valves.” Cell Proliferation 45 (4): 378–385.
Chicago author-date (all authors)
Somers, Pamela, Lieselot Robyns, Evelien Nollet, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Platelet Gel Supernatant as a Potential Tool to Repopulate Acellular Heart Valves.” Cell Proliferation 45 (4): 378–385.
Vancouver
1.
Somers P, Robyns L, Nollet E, De Somer F, Cornelissen M, Thierens H, et al. Platelet gel supernatant as a potential tool to repopulate acellular heart valves. CELL PROLIFERATION. 2012;45(4):378–85.
IEEE
[1]
P. Somers et al., “Platelet gel supernatant as a potential tool to repopulate acellular heart valves,” CELL PROLIFERATION, vol. 45, no. 4, pp. 378–385, 2012.
@article{2129176,
  abstract     = {Objective : The aim of this study was to repopulate decellularized heart valve matrices with ovine mesenchymal stem cells (oMSCs) by the use of platelet gel (PG) supernatant, a storage vehicle for growth factors.
Methods : oMSCs were exposed to different concentrations of PG-released supernatant and cell proliferation was evaluated using the MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. A quantitative sandwich enzyme immunoassay was used to examine amounts of bFGF and TGF-beta 1 in the PG supernatant. Repopulation of acellular heart valve matrices was stimulated by seeding matrices with oMSCs supplemented with the PG supernatant.
Results : The most significant increase in proliferation induced by PG supernatant appeared at 1x105plts/ml concentration. Higher concentrations evoked reduction of the stimulatory process. oMSC motility was most significantly stimulated at 1x106plts/ml. Stimulating invasiveness of oMSCs needed the much higher concentration of 2x106plts/ml. Immunoassays revealed that sheep PG supernatant contains 184.8pg/ml bFGF and 60.5ng/ml TGF-beta 1. Moreover, repopulation of acellular heart valve matrices was significantly enhanced by PG supernatant addition and resulted in upregulation of the myofibroblast marker alpha-smooth muscle actin.
Conclusions : Growth factors released from platelets had the potential to induce cell repopulation in a heart valve tissue engineering procedure, through stimulation of mesenchymal stem-cell migration and invasion.},
  author       = {Somers, Pamela and Robyns, Lieselot and Nollet, Evelien and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  issn         = {0960-7722},
  journal      = {CELL PROLIFERATION},
  keywords     = {HUMAN ENDOTHELIAL-CELLS,MESENCHYMAL STEM-CELLS,GROWTH-FACTOR LEVELS,RICH PLASMA,CLINICAL-APPLICATIONS,PROLIFERATION,DIFFERENTIATION,IDENTIFICATION,BLOOD},
  language     = {eng},
  number       = {4},
  pages        = {378--385},
  title        = {Platelet gel supernatant as a potential tool to repopulate acellular heart valves},
  url          = {http://dx.doi.org/10.1111/j.1365-2184.2012.00830.x},
  volume       = {45},
  year         = {2012},
}

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