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Platelet gel supernatant as a potential tool to repopulate acellular heart valves

Pamela Somers UGent, Lieselot Robyns UGent, Evelien Nollet UGent, Filip De Somer UGent, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2012) CELL PROLIFERATION. 45(4). p.378-385
abstract
Objective : The aim of this study was to repopulate decellularized heart valve matrices with ovine mesenchymal stem cells (oMSCs) by the use of platelet gel (PG) supernatant, a storage vehicle for growth factors. Methods : oMSCs were exposed to different concentrations of PG-released supernatant and cell proliferation was evaluated using the MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. A quantitative sandwich enzyme immunoassay was used to examine amounts of bFGF and TGF-beta 1 in the PG supernatant. Repopulation of acellular heart valve matrices was stimulated by seeding matrices with oMSCs supplemented with the PG supernatant. Results : The most significant increase in proliferation induced by PG supernatant appeared at 1x105plts/ml concentration. Higher concentrations evoked reduction of the stimulatory process. oMSC motility was most significantly stimulated at 1x106plts/ml. Stimulating invasiveness of oMSCs needed the much higher concentration of 2x106plts/ml. Immunoassays revealed that sheep PG supernatant contains 184.8pg/ml bFGF and 60.5ng/ml TGF-beta 1. Moreover, repopulation of acellular heart valve matrices was significantly enhanced by PG supernatant addition and resulted in upregulation of the myofibroblast marker alpha-smooth muscle actin. Conclusions : Growth factors released from platelets had the potential to induce cell repopulation in a heart valve tissue engineering procedure, through stimulation of mesenchymal stem-cell migration and invasion.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HUMAN ENDOTHELIAL-CELLS, MESENCHYMAL STEM-CELLS, GROWTH-FACTOR LEVELS, RICH PLASMA, CLINICAL-APPLICATIONS, PROLIFERATION, DIFFERENTIATION, IDENTIFICATION, BLOOD
journal title
CELL PROLIFERATION
Cell Prolif.
volume
45
issue
4
pages
378 - 385
Web of Science type
Article
Web of Science id
000305693000009
JCR category
CELL BIOLOGY
JCR impact factor
2.265 (2012)
JCR rank
129/181 (2012)
JCR quartile
3 (2012)
ISSN
0960-7722
DOI
10.1111/j.1365-2184.2012.00830.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2129176
handle
http://hdl.handle.net/1854/LU-2129176
date created
2012-06-01 15:00:44
date last changed
2012-09-20 14:04:22
@article{2129176,
  abstract     = {Objective : The aim of this study was to repopulate decellularized heart valve matrices with ovine mesenchymal stem cells (oMSCs) by the use of platelet gel (PG) supernatant, a storage vehicle for growth factors.
Methods : oMSCs were exposed to different concentrations of PG-released supernatant and cell proliferation was evaluated using the MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. A quantitative sandwich enzyme immunoassay was used to examine amounts of bFGF and TGF-beta 1 in the PG supernatant. Repopulation of acellular heart valve matrices was stimulated by seeding matrices with oMSCs supplemented with the PG supernatant.
Results : The most significant increase in proliferation induced by PG supernatant appeared at 1x105plts/ml concentration. Higher concentrations evoked reduction of the stimulatory process. oMSC motility was most significantly stimulated at 1x106plts/ml. Stimulating invasiveness of oMSCs needed the much higher concentration of 2x106plts/ml. Immunoassays revealed that sheep PG supernatant contains 184.8pg/ml bFGF and 60.5ng/ml TGF-beta 1. Moreover, repopulation of acellular heart valve matrices was significantly enhanced by PG supernatant addition and resulted in upregulation of the myofibroblast marker alpha-smooth muscle actin.
Conclusions : Growth factors released from platelets had the potential to induce cell repopulation in a heart valve tissue engineering procedure, through stimulation of mesenchymal stem-cell migration and invasion.},
  author       = {Somers, Pamela and Robyns, Lieselot and Nollet, Evelien and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  issn         = {0960-7722},
  journal      = {CELL PROLIFERATION},
  keyword      = {HUMAN ENDOTHELIAL-CELLS,MESENCHYMAL STEM-CELLS,GROWTH-FACTOR LEVELS,RICH PLASMA,CLINICAL-APPLICATIONS,PROLIFERATION,DIFFERENTIATION,IDENTIFICATION,BLOOD},
  language     = {eng},
  number       = {4},
  pages        = {378--385},
  title        = {Platelet gel supernatant as a potential tool to repopulate acellular heart valves},
  url          = {http://dx.doi.org/10.1111/j.1365-2184.2012.00830.x},
  volume       = {45},
  year         = {2012},
}

Chicago
Somers, Pamela, Lieselot Robyns, Evelien Nollet, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Platelet Gel Supernatant as a Potential Tool to Repopulate Acellular Heart Valves.” Cell Proliferation 45 (4): 378–385.
APA
Somers, P., Robyns, L., Nollet, E., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). Platelet gel supernatant as a potential tool to repopulate acellular heart valves. CELL PROLIFERATION, 45(4), 378–385.
Vancouver
1.
Somers P, Robyns L, Nollet E, De Somer F, Cornelissen M, Thierens H, et al. Platelet gel supernatant as a potential tool to repopulate acellular heart valves. CELL PROLIFERATION. 2012;45(4):378–85.
MLA
Somers, Pamela, Lieselot Robyns, Evelien Nollet, et al. “Platelet Gel Supernatant as a Potential Tool to Repopulate Acellular Heart Valves.” CELL PROLIFERATION 45.4 (2012): 378–385. Print.