Advanced search

Sticky platelet gel matrices in heart valve tissue engineering

Pamela Somers (UGent) , Filip De Somer (UGent) , Maria Cornelissen (UGent) , Hubert Thierens (UGent) and Guido Van Nooten (UGent)
Author
Organization
Abstract
Objectives: Platelet gel (PG), a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation. The aim of this study was to load porcine matrices with the PG-rich growth factors and to evaluate the effect on MSC repopulation. Materials and Methods: Ovine MSCs were isolated from bone marrow, expanded and differentiated to adipose tissue, cartilage and bone. Porcine matrices were incubated with the PG. A quantitative sandwich enzyme immunoassay was used to examine the release of growth factors from the matrices. oMSC repopulation was stimulated by static and dynamic culture. Results: Ovine MSCs were shown to display the trilineage differentiation potential. The immunoassays revealed that PG-incubated matrices showed a sustained release of 56.28 pg/ml bFGF and 30.66 ng/ml TGF-β1. Dynamic culture induced ovine MSC invasion in growth factor loaded matrices. Conclusion: Incubating a porcine aortic valve matrix with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction within the matrix in order to optimize cellular repopulation.

Citation

Please use this url to cite or link to this publication:

Chicago
Somers, Pamela, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2011. “Sticky Platelet Gel Matrices in Heart Valve Tissue Engineering.” In Biofuture 2011, Abstracts.
APA
Somers, P., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2011). Sticky platelet gel matrices in heart valve tissue engineering. Biofuture 2011, Abstracts. Presented at the Biofuture 2011 : Young European biomaterial scientists designing a view for the future.
Vancouver
1.
Somers P, De Somer F, Cornelissen M, Thierens H, Van Nooten G. Sticky platelet gel matrices in heart valve tissue engineering. Biofuture 2011, Abstracts. 2011.
MLA
Somers, Pamela, Filip De Somer, Maria Cornelissen, et al. “Sticky Platelet Gel Matrices in Heart Valve Tissue Engineering.” Biofuture 2011, Abstracts. 2011. Print.
@inproceedings{2128973,
  abstract     = {Objectives: Platelet gel (PG), a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation. The aim of this study was to load porcine matrices with the PG-rich growth factors and to evaluate the effect on MSC repopulation.
Materials and Methods: Ovine MSCs were isolated from bone marrow, expanded and differentiated to adipose tissue, cartilage and bone. Porcine matrices were incubated with the PG. A quantitative sandwich enzyme immunoassay was used to examine the release of growth factors from the matrices. oMSC repopulation was stimulated by static and dynamic culture.
Results: Ovine MSCs were shown to display the trilineage differentiation potential. The immunoassays revealed that PG-incubated matrices showed a sustained release of 56.28 pg/ml bFGF and 30.66 ng/ml TGF-\ensuremath{\beta}1. Dynamic culture induced ovine MSC invasion in growth factor loaded matrices.
Conclusion: Incubating a porcine aortic valve matrix with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction within the matrix in order to optimize cellular repopulation.},
  author       = {Somers, Pamela and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  booktitle    = {Biofuture 2011, Abstracts},
  language     = {eng},
  location     = {Ghent, Belgium},
  title        = {Sticky platelet gel matrices in heart valve tissue engineering},
  year         = {2011},
}