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Bioactive porcine matrices in heart valve tissue engineering

Pamela Somers UGent, Filip De Somer UGent, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2011) Society for Heart Valve Disease and the Heart Valve Society of America, Joint meeting, Abstracts.
abstract
Background: Platelet-gel (PG) as a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration and repair. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation and matrix synthesis in vitro and, in turn, optimize scaffold incorporation in vivo. The aim of this study was to evaluate the influence of different PG concentrations on in vitro ovine mesenchymal stem cell (oMSC) proliferation, migration and invasion. Moreover, the effect of PG on the repopulation of porcine aortic matrices was investigated. Methods: oMSCs were isolated from bone marrow, expanded and differentiated to adipose tissue, cartilage and bone. oMSCs were exposed to different concentrations of supernatant (0-2x106 plt/mL). After a culture period of 72 hours, cellular proliferation was evaluated by an MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. Decellularized porcine aortic valve matrices were incubated with the PG. Release of growth factors from the matrices was evaluated by ELISA. MSC repopulation in vitro was evaluated after 3 to 7 days by light microscopy. Results: oMSCs were shown to display the trilineage differentiation potential. The supernatant was able to stimulate cell proliferation already at a minimum concentration of 8x104plt/mL. Increasing platelet concentrations (<5x105 plt/mL) significantly increased oMSC proliferation, motility and invasiveness in a dose-dependent matter. Higher platelet concentrations (5x105 – 1x106 plt/mL) did not stimulate these processes any further since a plateau was reached. At the highest concentrations, there was a tendency to an inversion in the effect on proliferation, migration and invasion with values similar to those of untreated control cells. PG-incubated matrices showed a release of growth factors. oMSC density on these scaffolds increased significantly after 3-7 days compared to non-incubated control matrices. Conclusion: Incubating a decellularized porcine aortic valve with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction within the aortic valve matrix in order to optimize cellular repopulation.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
unpublished
subject
in
Society for Heart Valve Disease and the Heart Valve Society of America, Joint meeting, Abstracts
conference name
Joint meeting of the Society for Heart Valve Disease and the Heart Valve Society of America
conference location
Barcelona, Spain
conference start
2011-06-25
conference end
2011-06-28
language
English
UGent publication?
yes
classification
C3
id
2128598
handle
http://hdl.handle.net/1854/LU-2128598
date created
2012-06-01 11:19:16
date last changed
2012-06-15 09:28:04
@inproceedings{2128598,
  abstract     = {Background: Platelet-gel (PG) as a storage vehicle of growth factors, can be considered for the application of growth factors in combination with mesenchymal stem cells (MSCs) to accelerate tissue regeneration and repair. Moreover, the addition of bioactive factors to porcine aortic valves could result in a more rapid repopulation and matrix synthesis in vitro and, in turn, optimize scaffold incorporation in vivo. The aim of this study was to evaluate the influence of different PG concentrations on in vitro ovine mesenchymal stem cell (oMSC) proliferation, migration and invasion. Moreover, the effect of PG on the repopulation of porcine aortic matrices was investigated.
Methods: oMSCs were isolated from bone marrow, expanded and differentiated to adipose tissue, cartilage and bone. oMSCs were exposed to different concentrations of supernatant (0-2x106 plt/mL). After a culture period of 72 hours, cellular proliferation was evaluated by an MTS assay. oMSC motility and invasiveness were assayed using a Boyden chamber. Decellularized porcine aortic valve matrices were incubated with the PG. Release of growth factors from the matrices was evaluated by ELISA.  MSC repopulation in vitro was evaluated after 3 to 7 days by light microscopy.
Results: oMSCs were shown to display the trilineage differentiation potential. The supernatant was able to stimulate cell proliferation already at a minimum concentration of 8x104plt/mL. Increasing platelet concentrations ({\textlangle}5x105 plt/mL) significantly increased oMSC proliferation, motility and invasiveness in a dose-dependent matter. Higher platelet concentrations (5x105 -- 1x106 plt/mL) did not stimulate these processes any further since a plateau was reached. At the highest concentrations, there was a tendency to an inversion in the effect on proliferation, migration and invasion with values similar to those of untreated control cells. PG-incubated matrices showed a release of growth factors. oMSC density on these scaffolds increased significantly after 3-7 days compared to non-incubated control matrices. 
Conclusion:  Incubating a decellularized porcine aortic valve with a PG concentrate creates a bioactive matrix. However, further fine-tuning of the PG concentration is necessary to take full advantage of platelet growth factor interaction within the aortic valve matrix in order to optimize cellular repopulation.},
  author       = {Somers, Pamela and De Somer, Filip and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  booktitle    = {Society for Heart Valve Disease and the Heart Valve Society of America, Joint meeting, Abstracts},
  language     = {eng},
  location     = {Barcelona, Spain},
  title        = {Bioactive porcine matrices in heart valve tissue engineering},
  year         = {2011},
}

Chicago
Somers, Pamela, Filip De Somer, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2011. “Bioactive Porcine Matrices in Heart Valve Tissue Engineering.” In Society for Heart Valve Disease and the Heart Valve Society of America, Joint Meeting, Abstracts.
APA
Somers, P., De Somer, F., Cornelissen, M., Thierens, H., & Van Nooten, G. (2011). Bioactive porcine matrices in heart valve tissue engineering. Society for Heart Valve Disease and the Heart Valve Society of America, Joint meeting, Abstracts. Presented at the Joint meeting of the Society for Heart Valve Disease and the Heart Valve Society of America.
Vancouver
1.
Somers P, De Somer F, Cornelissen M, Thierens H, Van Nooten G. Bioactive porcine matrices in heart valve tissue engineering. Society for Heart Valve Disease and the Heart Valve Society of America, Joint meeting, Abstracts. 2011.
MLA
Somers, Pamela, Filip De Somer, Maria Cornelissen, et al. “Bioactive Porcine Matrices in Heart Valve Tissue Engineering.” Society for Heart Valve Disease and the Heart Valve Society of America, Joint Meeting, Abstracts. 2011. Print.