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Measuring absolute number concentrations of nanoparticles using single-particle tracking

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Center for nano- and biophotonics (NB-Photonics)
Abstract
Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle number concentration in Brownian particle dispersions using SPT based on a model for the trajectory length distribution of particles to estimate the detection region volume. The resulting method is independent of precalibration reference measurements, and robust with respect to image processing settings. Experimentally estimated concentrations of different dilutions of 0.19- and 0.52-mu m polymer nanospheres are in excellent agreement with estimates computed from the concentrations of the stock solutions.
Keywords
DELIVERY, SIZE

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Citation

Please use this url to cite or link to this publication:

Chicago
Röding, Magnus, Hendrik Deschout, Kevin Braeckmans, and Mats Rudemo. 2011. “Measuring Absolute Number Concentrations of Nanoparticles Using Single-particle Tracking.” Physical Review E 84 (3).
APA
Röding, Magnus, Deschout, H., Braeckmans, K., & Rudemo, M. (2011). Measuring absolute number concentrations of nanoparticles using single-particle tracking. PHYSICAL REVIEW E, 84(3).
Vancouver
1.
Röding M, Deschout H, Braeckmans K, Rudemo M. Measuring absolute number concentrations of nanoparticles using single-particle tracking. PHYSICAL REVIEW E. 2011;84(3).
MLA
Röding, Magnus, Hendrik Deschout, Kevin Braeckmans, et al. “Measuring Absolute Number Concentrations of Nanoparticles Using Single-particle Tracking.” PHYSICAL REVIEW E 84.3 (2011): n. pag. Print.
@article{2128457,
  abstract     = {Single-particle tracking (SPT) microscopy is increasingly used to characterize nanoparticulate systems. We introduce a concept for estimation of particle number concentration in Brownian particle dispersions using SPT based on a model for the trajectory length distribution of particles to estimate the detection region volume. The resulting method is independent of precalibration reference measurements, and robust with respect to image processing settings. Experimentally estimated concentrations of different dilutions of 0.19- and 0.52-mu m polymer nanospheres are in excellent agreement with estimates computed from the concentrations of the stock solutions.},
  articleno    = {031920},
  author       = {R{\"o}ding, Magnus and Deschout, Hendrik and Braeckmans, Kevin and Rudemo, Mats},
  issn         = {1539-3755},
  journal      = {PHYSICAL REVIEW E},
  keyword      = {DELIVERY,SIZE},
  language     = {eng},
  number       = {3},
  pages        = {9},
  title        = {Measuring absolute number concentrations of nanoparticles using single-particle tracking},
  url          = {http://dx.doi.org/10.1103/PhysRevE.84.031920},
  volume       = {84},
  year         = {2011},
}

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