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A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine

(2010) JOURNAL OF SEPARATION SCIENCE. 33(17-18). p.2654-2662
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Abstract
Fentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3-methylfentanyl are potent, short-acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl-type compounds in plasma and urine is presented, consisting of a liquid-liquid extraction followed by a LC-MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl-d(5) and norfentanyl-d(5) were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3-methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2-50 ng/mL for sufentanil and 0.1-50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.
Keywords
GAS-CHROMATOGRAPHY, SOLID-PHASE MICROEXTRACTION, ULTRAVIOLET DETECTION, MAJOR METABOLITES, SUFENTANIL, ALFENTANIL, IDENTIFICATION, REMIFENTANIL, PERFORMANCE LIQUID-CHROMATOGRAPHY, Fentanyl, Quantification, Toxicology, CHROMATOGRAPHY-MASS SPECTROMETRY, LC-MS/MS, Fentanyl-like drugs

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Citation

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Chicago
Cooreman, Sarah, Christa Deprez, Frank Martens, Jan Van Bocxlaer, and Kathleen Croes. 2010. “A Comprehensive LC-MS-based Quantitative Analysis of Fentanyl-like Drugs in Plasma and Urine.” Journal of Separation Science 33 (17-18): 2654–2662.
APA
Cooreman, S., Deprez, C., Martens, F., Van Bocxlaer, J., & Croes, K. (2010). A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine. JOURNAL OF SEPARATION SCIENCE, 33(17-18), 2654–2662.
Vancouver
1.
Cooreman S, Deprez C, Martens F, Van Bocxlaer J, Croes K. A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine. JOURNAL OF SEPARATION SCIENCE. 2010;33(17-18):2654–62.
MLA
Cooreman, Sarah, Christa Deprez, Frank Martens, et al. “A Comprehensive LC-MS-based Quantitative Analysis of Fentanyl-like Drugs in Plasma and Urine.” JOURNAL OF SEPARATION SCIENCE 33.17-18 (2010): 2654–2662. Print.
@article{2128370,
  abstract     = {Fentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3-methylfentanyl are potent, short-acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl-type compounds in plasma and urine is presented, consisting of a liquid-liquid extraction followed by a LC-MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl-d(5) and norfentanyl-d(5) were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3-methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2-50 ng/mL for sufentanil and 0.1-50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15\%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15\% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.},
  author       = {Cooreman, Sarah and Deprez, Christa and Martens, Frank and Van Bocxlaer, Jan and Croes, Kathleen},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {GAS-CHROMATOGRAPHY,SOLID-PHASE MICROEXTRACTION,ULTRAVIOLET DETECTION,MAJOR METABOLITES,SUFENTANIL,ALFENTANIL,IDENTIFICATION,REMIFENTANIL,PERFORMANCE LIQUID-CHROMATOGRAPHY,Fentanyl,Quantification,Toxicology,CHROMATOGRAPHY-MASS SPECTROMETRY,LC-MS/MS,Fentanyl-like drugs},
  language     = {eng},
  number       = {17-18},
  pages        = {2654--2662},
  title        = {A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine},
  url          = {http://dx.doi.org/10.1002/jssc.201000330},
  volume       = {33},
  year         = {2010},
}

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