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A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine

Sarah Cooreman, Christa Deprez, Frank Martens, Jan Van Bocxlaer UGent and Kathleen Croes (2010) JOURNAL OF SEPARATION SCIENCE. 33(17-18). p.2654-2662
abstract
Fentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3-methylfentanyl are potent, short-acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl-type compounds in plasma and urine is presented, consisting of a liquid-liquid extraction followed by a LC-MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl-d(5) and norfentanyl-d(5) were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3-methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2-50 ng/mL for sufentanil and 0.1-50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GAS-CHROMATOGRAPHY, SOLID-PHASE MICROEXTRACTION, ULTRAVIOLET DETECTION, MAJOR METABOLITES, SUFENTANIL, ALFENTANIL, IDENTIFICATION, REMIFENTANIL, PERFORMANCE LIQUID-CHROMATOGRAPHY, Fentanyl, Quantification, Toxicology, CHROMATOGRAPHY-MASS SPECTROMETRY, LC-MS/MS, Fentanyl-like drugs
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
33
issue
17-18
pages
2654 - 2662
Web of Science type
Article
Web of Science id
000282612900013
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.631 (2010)
JCR rank
23/71 (2010)
JCR quartile
2 (2010)
ISSN
1615-9306
DOI
10.1002/jssc.201000330
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2128370
handle
http://hdl.handle.net/1854/LU-2128370
date created
2012-06-01 10:42:38
date last changed
2016-12-19 15:42:57
@article{2128370,
  abstract     = {Fentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3-methylfentanyl are potent, short-acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl-type compounds in plasma and urine is presented, consisting of a liquid-liquid extraction followed by a LC-MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl-d(5) and norfentanyl-d(5) were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3-methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2-50 ng/mL for sufentanil and 0.1-50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15\%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15\% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.},
  author       = {Cooreman, Sarah and Deprez, Christa and Martens, Frank and Van Bocxlaer, Jan and Croes, Kathleen},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {GAS-CHROMATOGRAPHY,SOLID-PHASE MICROEXTRACTION,ULTRAVIOLET DETECTION,MAJOR METABOLITES,SUFENTANIL,ALFENTANIL,IDENTIFICATION,REMIFENTANIL,PERFORMANCE LIQUID-CHROMATOGRAPHY,Fentanyl,Quantification,Toxicology,CHROMATOGRAPHY-MASS SPECTROMETRY,LC-MS/MS,Fentanyl-like drugs},
  language     = {eng},
  number       = {17-18},
  pages        = {2654--2662},
  title        = {A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine},
  url          = {http://dx.doi.org/10.1002/jssc.201000330},
  volume       = {33},
  year         = {2010},
}

Chicago
Cooreman, Sarah, Christa Deprez, Frank Martens, Jan Van Bocxlaer, and Kathleen Croes. 2010. “A Comprehensive LC-MS-based Quantitative Analysis of Fentanyl-like Drugs in Plasma and Urine.” Journal of Separation Science 33 (17-18): 2654–2662.
APA
Cooreman, S., Deprez, C., Martens, F., Van Bocxlaer, J., & Croes, K. (2010). A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine. JOURNAL OF SEPARATION SCIENCE, 33(17-18), 2654–2662.
Vancouver
1.
Cooreman S, Deprez C, Martens F, Van Bocxlaer J, Croes K. A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine. JOURNAL OF SEPARATION SCIENCE. 2010;33(17-18):2654–62.
MLA
Cooreman, Sarah, Christa Deprez, Frank Martens, et al. “A Comprehensive LC-MS-based Quantitative Analysis of Fentanyl-like Drugs in Plasma and Urine.” JOURNAL OF SEPARATION SCIENCE 33.17-18 (2010): 2654–2662. Print.