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Adipose derived-stem cells as source of endothelial cells for cardiac tissue engineering

Pamela Somers UGent, Francesca Colazzo, Ivan Carubelli, A Chester, Maria Cornelissen UGent, Hubert Thierens UGent and Guido Van Nooten UGent (2012) CARDIOLOGY. 121(2). p.138-138
abstract
Objectives: Tissue engineering approaches hold the promise to create non-thrombogenic biological substitutes for cardiovascular tissues and devices. The objective of this study was to investigate the endothelial differentiation potential of adipose-derived stem cells (ADSCs) and evaluate their phenotype after matrix interaction. Methods: ADSCs were isolated from human adipose tissue and differentiated into an endothelial phenotype by means of vascular endothelial growth factor (VEGF) and/or shear stress. Afterwards, endothelial cells were seeded onto collagen and acellular aortic valve matrices. Seeded matrices were exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF. After 7 days, endothelial phenotype of the cells on the matrices was investigated. Results: Endothelial differentiation was obtained after 7 days culture. Approximately 70% of the cells was positive for vWF and CD31. Moreover, the expression of the myofibroblastic marker α-SMA was significantly decreased. The only culture condition which preserved endothelial phenotype of the cells on the matrices after 7 days was the application of shear stress in combination with VEGF addition (shear stress+VEGF). Conclusions: These results suggest that ADSCs can differentiate into endothelial cells by use of VEGF and shear stress. Moreover, endothelial phenotype is maintained when seeded onto scaffolds, solely in the presence of shear stress+VEGF.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
CARDIOLOGY
Cardiology
volume
121
issue
2
pages
138 - 138
conference name
Valves in the Heart of the Big Apple VII : Evaluation and management of valvular heart diseases
conference location
New York, NY, USA
conference start
2012-04-12
conference end
2012-04-14
Web of Science type
Meeting Abstract
Web of Science id
000302133800109
JCR category
CARDIAC & CARDIOVASCULAR SYSTEMS
JCR impact factor
1.519 (2012)
JCR rank
70/120 (2012)
JCR quartile
3 (2012)
ISSN
0008-6312
language
English
UGent publication?
yes
classification
C3
id
2128282
handle
http://hdl.handle.net/1854/LU-2128282
date created
2012-06-01 10:29:44
date last changed
2012-06-15 14:44:13
@inproceedings{2128282,
  abstract     = {Objectives: Tissue engineering approaches hold the promise to create non-thrombogenic biological substitutes for cardiovascular tissues and devices. The objective of this study was to investigate the endothelial differentiation potential of adipose-derived stem cells (ADSCs) and evaluate their phenotype after matrix interaction.
Methods: ADSCs were isolated from human adipose tissue and differentiated into an endothelial phenotype by means of vascular endothelial growth factor (VEGF) and/or shear stress. Afterwards, endothelial cells were seeded onto collagen and acellular aortic valve matrices. Seeded matrices were exposed to 4 culture conditions: shear stress+VEGF; shear stress-VEGF; static+VEGF and static-VEGF.  After 7 days, endothelial phenotype of the cells on the matrices was investigated.
Results: Endothelial differentiation was obtained after 7 days culture. Approximately 70\% of the cells was positive for vWF and CD31. Moreover, the expression of the myofibroblastic marker \ensuremath{\alpha}-SMA was significantly decreased. The only culture condition which preserved endothelial phenotype of the cells on the matrices after 7 days was the application of shear stress in combination with VEGF addition (shear stress+VEGF).
Conclusions: These results suggest that ADSCs can differentiate into endothelial cells by use of  VEGF and shear stress. Moreover, endothelial phenotype is maintained when seeded onto scaffolds, solely in the presence of shear stress+VEGF.},
  author       = {Somers, Pamela and Colazzo, Francesca and Carubelli, Ivan  and Chester, A and Cornelissen, Maria and Thierens, Hubert and Van Nooten, Guido},
  booktitle    = {CARDIOLOGY},
  issn         = {0008-6312},
  language     = {eng},
  location     = {New York, NY, USA},
  number       = {2},
  pages        = {138--138},
  title        = {Adipose derived-stem cells as source of endothelial cells for cardiac tissue engineering},
  volume       = {121},
  year         = {2012},
}

Chicago
Somers, Pamela, Francesca Colazzo, Ivan Carubelli, A Chester, Maria Cornelissen, Hubert Thierens, and Guido Van Nooten. 2012. “Adipose Derived-stem Cells as Source of Endothelial Cells for Cardiac Tissue Engineering.” In Cardiology, 121:138–138.
APA
Somers, P., Colazzo, F., Carubelli, I., Chester, A., Cornelissen, M., Thierens, H., & Van Nooten, G. (2012). Adipose derived-stem cells as source of endothelial cells for cardiac tissue engineering. CARDIOLOGY (Vol. 121, pp. 138–138). Presented at the Valves in the Heart of the Big Apple VII : Evaluation and management of valvular heart diseases.
Vancouver
1.
Somers P, Colazzo F, Carubelli I, Chester A, Cornelissen M, Thierens H, et al. Adipose derived-stem cells as source of endothelial cells for cardiac tissue engineering. CARDIOLOGY. 2012. p. 138–138.
MLA
Somers, Pamela, Francesca Colazzo, Ivan Carubelli, et al. “Adipose Derived-stem Cells as Source of Endothelial Cells for Cardiac Tissue Engineering.” Cardiology. Vol. 121. 2012. 138–138. Print.