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Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum

Evi Debruyne UGent, MARIJN SPEECKAERT UGent, Yannick Vande Weygaerde UGent and Joris Delanghe UGent (2011) CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 49(11). p.1855-1860
abstract
Background: Gc-globulin is a polymorphic protein with three phenotypes: Gc 1-1, Gc 2-1 and Gc 2-2. Deglycosylation of Gc-globulin results in a Gc-macrophage activating factor (Gc-MAF). This study investigated the potential of MAF as a tumour marker and the influence of Gc-phenotypes on serum MAF-concentrations. Methods: Gc-phenotype of 98 healthy individuals and 60 cancer patients was determined. MAF-levels of healthy individuals and cancer patients were analysed according to their Gc-phenotype using a Helix pomatia agglutinin-based ELISA. ROC curves analysed the efficiency of MAF as a tumour marker. Results: MAF-levels between controls and patients were significantly different (p<0.001). No phenotypic differences were found in the patients. In comparison with the controls, MAF-values were significantly lower in cancer patients carrying Gc 1-1 (p<0.01) and Gc 2-1 (p<0.001). No difference was observed in Gc 2-2 phenotype. Diagnostic accuracy of MAF as a tumour marker also demonstrated pronounced differences between Gc-phenotypes. Conclusions: Gc-phenotype is a confounding factor when interpreting MAF-data. The value of MAF as a tumour marker varies according to phenotype. Future studies on MAF will have to consider the effect of Gc-phenotype.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
tumour marker, phenotype, D-BINDING PROTEIN, GROUP-SPECIFIC COMPONENT, CANCER-PATIENTS, TUMOR-GROWTH, DEGLYCOSYLATION, IMMUNOSUPPRESSION, GALACTOSIDASE, PRECURSOR, SYSTEM, CELLS, Helix pomatia agglutinin, Gc-MAF, lectin-based ELISA, Gc-globulin
journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Clin. Chem. Lab. Med.
volume
49
issue
11
pages
1855 - 1860
Web of Science type
Article
Web of Science id
000296772300013
JCR category
MEDICAL LABORATORY TECHNOLOGY
JCR impact factor
2.15 (2011)
JCR rank
10/32 (2011)
JCR quartile
2 (2011)
ISSN
1434-6621
DOI
10.1515/CCLM.2011.676
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2127347
handle
http://hdl.handle.net/1854/LU-2127347
date created
2012-05-31 22:12:05
date last changed
2012-06-04 13:13:21
@article{2127347,
  abstract     = {Background: Gc-globulin is a polymorphic protein with three phenotypes: Gc 1-1, Gc 2-1 and Gc 2-2. Deglycosylation of Gc-globulin results in a Gc-macrophage activating factor (Gc-MAF). This study investigated the potential of MAF as a tumour marker and the influence of Gc-phenotypes on serum MAF-concentrations. 
Methods: Gc-phenotype of 98 healthy individuals and 60 cancer patients was determined. MAF-levels of healthy individuals and cancer patients were analysed according to their Gc-phenotype using a Helix pomatia agglutinin-based ELISA. ROC curves analysed the efficiency of MAF as a tumour marker. 
Results: MAF-levels between controls and patients were significantly different (p{\textlangle}0.001). No phenotypic differences were found in the patients. In comparison with the controls, MAF-values were significantly lower in cancer patients carrying Gc 1-1 (p{\textlangle}0.01) and Gc 2-1 (p{\textlangle}0.001). No difference was observed in Gc 2-2 phenotype. Diagnostic accuracy of MAF as a tumour marker also demonstrated pronounced differences between Gc-phenotypes. 
Conclusions: Gc-phenotype is a confounding factor when interpreting MAF-data. The value of MAF as a tumour marker varies according to phenotype. Future studies on MAF will have to consider the effect of Gc-phenotype.},
  author       = {Debruyne, Evi and SPEECKAERT, MARIJN and Vande Weygaerde, Yannick and Delanghe, Joris},
  issn         = {1434-6621},
  journal      = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
  keyword      = {tumour marker,phenotype,D-BINDING PROTEIN,GROUP-SPECIFIC COMPONENT,CANCER-PATIENTS,TUMOR-GROWTH,DEGLYCOSYLATION,IMMUNOSUPPRESSION,GALACTOSIDASE,PRECURSOR,SYSTEM,CELLS,Helix pomatia agglutinin,Gc-MAF,lectin-based ELISA,Gc-globulin},
  language     = {eng},
  number       = {11},
  pages        = {1855--1860},
  title        = {Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum},
  url          = {http://dx.doi.org/10.1515/CCLM.2011.676},
  volume       = {49},
  year         = {2011},
}

Chicago
Debruyne, Evi, MARIJN SPEECKAERT, Yannick Vande Weygaerde, and Joris Delanghe. 2011. “Phenotype of Gc-globulin Influences the Macrophage Activating Factor (MAF) Levels in Serum.” Clinical Chemistry and Laboratory Medicine 49 (11): 1855–1860.
APA
Debruyne, E., SPEECKAERT, M., Vande Weygaerde, Y., & Delanghe, J. (2011). Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 49(11), 1855–1860.
Vancouver
1.
Debruyne E, SPEECKAERT M, Vande Weygaerde Y, Delanghe J. Phenotype of Gc-globulin influences the macrophage activating factor (MAF) levels in serum. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2011;49(11):1855–60.
MLA
Debruyne, Evi, MARIJN SPEECKAERT, Yannick Vande Weygaerde, et al. “Phenotype of Gc-globulin Influences the Macrophage Activating Factor (MAF) Levels in Serum.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 49.11 (2011): 1855–1860. Print.