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Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure

Tim Verslycke, Sofie Poelmans, Katia De Wasch, Jordy Vercauteren, Christophe Devos, Luc Moens UGent, Patrick Sandra UGent, Hubert De Brabander UGent and Colin Janssen UGent (2003) ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. 22(9). p.2030-2036
abstract
Current evidence suggests that the biocide tributyltin (TBT) causes the development of imposex, a state of pseudohermaphrodism in which females exhibit functional secondary male characteristics, by altering the biotransformation or elimination of testosterone. Imposex in gastropods following TBT exposure is the most complete example of the effects of an endocrine disrupter on marine invertebrates. Previous studies have demonstrated that the estuarine mysid Neomysis integer converts testosterone into multiple polar and nonpolar metabolites resulting from both phase I and phase II biotransformations. In this study, the effects of TBT chloride (TBTCl) on the phase I and II testosterone metabolism of N. integer were evaluated. The TBTCl was highly toxic to N. integer (96-h median lethal concentration [LC50] of 164 ng/L). To assess the effects on testosterone metabolism, mysids were exposed for 96 h to different concentrations of TBTCl (control, 10, 100, and 1,000 ng/L), and testosterone elimination as polar hydroxylated, nonpolar oxido-reduced, and glucose- and sulfate-conjugated metabolites was examined. The TBTCl differentially affected testosterone metabolism. The effect of TBTCl on phase I metabolism was unclear and has been shown to vary among species, likely depending on the inducibility or presence of certain P450 isozyme families. Reductase activity and metabolic androgenization were induced in the 10-ng/L treatment, whereas higher concentrations resulted in a reduction of sulfate conjugation. The exact mechanisms underlying TBT-induced imposex and alterations in the steroid metabolism need to be further elucidated.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IN-VIVO, TOXICITY, MUD SNAIL, INDUCED IMPOSEX, NUCELLA-LAPILLUS, ILYANASSA-OBSOLETA, ENDOCRINE DISRUPTION, DAPHNIA-MAGNA, biomarker, endocrine disruption, tributyltin, testosterone metabolism, neomysis integer, BIOTRANSFORMATION, MARINE
journal title
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Environ. Toxicol. Chem.
volume
22
issue
9
pages
2030 - 2036
Web of Science type
Article
Web of Science id
000185639400010
JCR category
ENVIRONMENTAL SCIENCES
JCR impact factor
2.429 (2003)
JCR rank
10/131 (2003)
JCR quartile
1 (2003)
ISSN
0730-7268
DOI
10.1002/etc.5620220910
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
212718
handle
http://hdl.handle.net/1854/LU-212718
date created
2004-04-26 14:19:00
date last changed
2011-08-16 14:36:14
@article{212718,
  abstract     = {Current evidence suggests that the biocide tributyltin (TBT) causes the development of imposex, a state of pseudohermaphrodism in which females exhibit functional secondary male characteristics, by altering the biotransformation or elimination of testosterone. Imposex in gastropods following TBT exposure is the most complete example of the effects of an endocrine disrupter on marine invertebrates. Previous studies have demonstrated that the estuarine mysid Neomysis integer converts testosterone into multiple polar and nonpolar metabolites resulting from both phase I and phase II biotransformations. In this study, the effects of TBT chloride (TBTCl) on the phase I and II testosterone metabolism of N. integer were evaluated. The TBTCl was highly toxic to N. integer (96-h median lethal concentration [LC50] of 164 ng/L). To assess the effects on testosterone metabolism, mysids were exposed for 96 h to different concentrations of TBTCl (control, 10, 100, and 1,000 ng/L), and testosterone elimination as polar hydroxylated, nonpolar oxido-reduced, and glucose- and sulfate-conjugated metabolites was examined. The TBTCl differentially affected testosterone metabolism. The effect of TBTCl on phase I metabolism was unclear and has been shown to vary among species, likely depending on the inducibility or presence of certain P450 isozyme families. Reductase activity and metabolic androgenization were induced in the 10-ng/L treatment, whereas higher concentrations resulted in a reduction of sulfate conjugation. The exact mechanisms underlying TBT-induced imposex and alterations in the steroid metabolism need to be further elucidated.},
  author       = {Verslycke, Tim and Poelmans, Sofie and De Wasch, Katia and Vercauteren, Jordy and Devos, Christophe and Moens, Luc and Sandra, Patrick and De Brabander, Hubert and Janssen, Colin},
  issn         = {0730-7268},
  journal      = {ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY},
  keyword      = {IN-VIVO,TOXICITY,MUD SNAIL,INDUCED IMPOSEX,NUCELLA-LAPILLUS,ILYANASSA-OBSOLETA,ENDOCRINE DISRUPTION,DAPHNIA-MAGNA,biomarker,endocrine disruption,tributyltin,testosterone metabolism,neomysis integer,BIOTRANSFORMATION,MARINE},
  language     = {eng},
  number       = {9},
  pages        = {2030--2036},
  title        = {Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure},
  url          = {http://dx.doi.org/10.1002/etc.5620220910},
  volume       = {22},
  year         = {2003},
}

Chicago
Verslycke, Tim, Sofie Poelmans, Katia De Wasch, Jordy Vercauteren, Christophe Devos, Luc Moens, Patrick Sandra, Hubert De Brabander, and Colin Janssen. 2003. “Testosterone Metabolism in the Estuarine Mysid Neomysis Integer (Crustacea: Mysidacea) Following Tributyltin Exposure.” Environmental Toxicology and Chemistry 22 (9): 2030–2036.
APA
Verslycke, T., Poelmans, S., De Wasch, K., Vercauteren, J., Devos, C., Moens, L., Sandra, P., et al. (2003). Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 22(9), 2030–2036.
Vancouver
1.
Verslycke T, Poelmans S, De Wasch K, Vercauteren J, Devos C, Moens L, et al. Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following tributyltin exposure. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. 2003;22(9):2030–6.
MLA
Verslycke, Tim, Sofie Poelmans, Katia De Wasch, et al. “Testosterone Metabolism in the Estuarine Mysid Neomysis Integer (Crustacea: Mysidacea) Following Tributyltin Exposure.” ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 22.9 (2003): 2030–2036. Print.