An intrinsic mechanism of corticogenesis from embryonic stem cells
- Author
- Nicolas Gaspard, Tristan Bouschet, Raphael Hourez, Jordane Dimidschstein, Gilles Naeije, Jelle van den Ameele (UGent) , Ira Espuny-Camacho, Adèle Herpoel, Lara Passante, Serge N Schiffmann, Afsaneh Gaillard and Pierre Vanderhaeghen
- Organization
- Abstract
- The cerebral cortex develops through the coordinated generation of dozens of neuronal subtypes, but the mechanisms involved remain unclear. Here we show that mouse embryonic stem cells, cultured without any morphogen but in the presence of a sonic hedgehog inhibitor, recapitulate in vitro the major milestones of cortical development, leading to the sequential generation of a diverse repertoire of neurons that display most salient features of genuine cortical pyramidal neurons. When grafted into the cerebral cortex, these neurons develop patterns of axonal projections corresponding to a wide range of cortical layers, but also to highly specific cortical areas, in particular visual and limbic areas, thereby demonstrating that the identity of a cortical area can be specified without any influence from the brain. The discovery of intrinsic corticogenesis sheds new light on the mechanisms of neuronal specification, and opens new avenues for the modelling and treatment of brain diseases.
- Keywords
- PROJECTION NEURON, CORTICAL DEVELOPMENT, DIRECTED DIFFERENTIATION, DEVELOPING CEREBRAL-CORTEX, RADIAL GLIA, SPECIFICATION, FATE, NEOCORTEX, TRANSPLANTATION, PRECURSORS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-2126027
- MLA
- Gaspard, Nicolas, et al. “An Intrinsic Mechanism of Corticogenesis from Embryonic Stem Cells.” NATURE, vol. 455, no. 7211, 2008, pp. 351–57, doi:10.1038/nature07287.
- APA
- Gaspard, N., Bouschet, T., Hourez, R., Dimidschstein, J., Naeije, G., van den Ameele, J., … Vanderhaeghen, P. (2008). An intrinsic mechanism of corticogenesis from embryonic stem cells. NATURE, 455(7211), 351–357. https://doi.org/10.1038/nature07287
- Chicago author-date
- Gaspard, Nicolas, Tristan Bouschet, Raphael Hourez, Jordane Dimidschstein, Gilles Naeije, Jelle van den Ameele, Ira Espuny-Camacho, et al. 2008. “An Intrinsic Mechanism of Corticogenesis from Embryonic Stem Cells.” NATURE 455 (7211): 351–57. https://doi.org/10.1038/nature07287.
- Chicago author-date (all authors)
- Gaspard, Nicolas, Tristan Bouschet, Raphael Hourez, Jordane Dimidschstein, Gilles Naeije, Jelle van den Ameele, Ira Espuny-Camacho, Adèle Herpoel, Lara Passante, Serge N Schiffmann, Afsaneh Gaillard, and Pierre Vanderhaeghen. 2008. “An Intrinsic Mechanism of Corticogenesis from Embryonic Stem Cells.” NATURE 455 (7211): 351–357. doi:10.1038/nature07287.
- Vancouver
- 1.Gaspard N, Bouschet T, Hourez R, Dimidschstein J, Naeije G, van den Ameele J, et al. An intrinsic mechanism of corticogenesis from embryonic stem cells. NATURE. 2008;455(7211):351–7.
- IEEE
- [1]N. Gaspard et al., “An intrinsic mechanism of corticogenesis from embryonic stem cells,” NATURE, vol. 455, no. 7211, pp. 351–357, 2008.
@article{2126027, abstract = {{The cerebral cortex develops through the coordinated generation of dozens of neuronal subtypes, but the mechanisms involved remain unclear. Here we show that mouse embryonic stem cells, cultured without any morphogen but in the presence of a sonic hedgehog inhibitor, recapitulate in vitro the major milestones of cortical development, leading to the sequential generation of a diverse repertoire of neurons that display most salient features of genuine cortical pyramidal neurons. When grafted into the cerebral cortex, these neurons develop patterns of axonal projections corresponding to a wide range of cortical layers, but also to highly specific cortical areas, in particular visual and limbic areas, thereby demonstrating that the identity of a cortical area can be specified without any influence from the brain. The discovery of intrinsic corticogenesis sheds new light on the mechanisms of neuronal specification, and opens new avenues for the modelling and treatment of brain diseases.}}, author = {{Gaspard, Nicolas and Bouschet, Tristan and Hourez, Raphael and Dimidschstein, Jordane and Naeije, Gilles and van den Ameele, Jelle and Espuny-Camacho, Ira and Herpoel, Adèle and Passante, Lara and Schiffmann, Serge N and Gaillard, Afsaneh and Vanderhaeghen, Pierre}}, issn = {{0028-0836}}, journal = {{NATURE}}, keywords = {{PROJECTION NEURON,CORTICAL DEVELOPMENT,DIRECTED DIFFERENTIATION,DEVELOPING CEREBRAL-CORTEX,RADIAL GLIA,SPECIFICATION,FATE,NEOCORTEX,TRANSPLANTATION,PRECURSORS}}, language = {{eng}}, number = {{7211}}, pages = {{351--357}}, title = {{An intrinsic mechanism of corticogenesis from embryonic stem cells}}, url = {{http://doi.org/10.1038/nature07287}}, volume = {{455}}, year = {{2008}}, }
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