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An intrinsic mechanism of corticogenesis from embryonic stem cells

Nicolas Gaspard, Tristan Bouschet, Raphael Hourez, Jordane Dimidschstein, Gilles Naeije, Jelle van den Ameele UGent, Ira Espuny-Camacho, Adèle Herpoel, Lara Passante and Serge N Schiffmann, et al. (2008) NATURE. 455(7211). p.351-357
abstract
The cerebral cortex develops through the coordinated generation of dozens of neuronal subtypes, but the mechanisms involved remain unclear. Here we show that mouse embryonic stem cells, cultured without any morphogen but in the presence of a sonic hedgehog inhibitor, recapitulate in vitro the major milestones of cortical development, leading to the sequential generation of a diverse repertoire of neurons that display most salient features of genuine cortical pyramidal neurons. When grafted into the cerebral cortex, these neurons develop patterns of axonal projections corresponding to a wide range of cortical layers, but also to highly specific cortical areas, in particular visual and limbic areas, thereby demonstrating that the identity of a cortical area can be specified without any influence from the brain. The discovery of intrinsic corticogenesis sheds new light on the mechanisms of neuronal specification, and opens new avenues for the modelling and treatment of brain diseases.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROJECTION NEURON, CORTICAL DEVELOPMENT, DIRECTED DIFFERENTIATION, DEVELOPING CEREBRAL-CORTEX, RADIAL GLIA, SPECIFICATION, FATE, NEOCORTEX, TRANSPLANTATION, PRECURSORS
journal title
NATURE
Nature
volume
455
issue
7211
pages
351 - 357
Web of Science type
Article
Web of Science id
000259265200036
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
31.434 (2008)
JCR rank
1/41 (2008)
JCR quartile
1 (2008)
ISSN
0028-0836
DOI
10.1038/nature07287
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2126027
handle
http://hdl.handle.net/1854/LU-2126027
date created
2012-05-31 21:47:01
date last changed
2012-06-13 15:09:36
@article{2126027,
  abstract     = {The cerebral cortex develops through the coordinated generation of dozens of neuronal subtypes, but the mechanisms involved remain unclear. Here we show that mouse embryonic stem cells, cultured without any morphogen but in the presence of a sonic hedgehog inhibitor, recapitulate in vitro the major milestones of cortical development, leading to the sequential generation of a diverse repertoire of neurons that display most salient features of genuine cortical pyramidal neurons. When grafted into the cerebral cortex, these neurons develop patterns of axonal projections corresponding to a wide range of cortical layers, but also to highly specific cortical areas, in particular visual and limbic areas, thereby demonstrating that the identity of a cortical area can be specified without any influence from the brain. The discovery of intrinsic corticogenesis sheds new light on the mechanisms of neuronal specification, and opens new avenues for the modelling and treatment of brain diseases.},
  author       = {Gaspard, Nicolas and Bouschet, Tristan and Hourez, Raphael and Dimidschstein, Jordane and Naeije, Gilles and van den Ameele, Jelle and Espuny-Camacho, Ira and Herpoel, Ad{\`e}le and Passante, Lara and Schiffmann, Serge N and Gaillard, Afsaneh and Vanderhaeghen, Pierre},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {PROJECTION NEURON,CORTICAL DEVELOPMENT,DIRECTED DIFFERENTIATION,DEVELOPING CEREBRAL-CORTEX,RADIAL GLIA,SPECIFICATION,FATE,NEOCORTEX,TRANSPLANTATION,PRECURSORS},
  language     = {eng},
  number       = {7211},
  pages        = {351--357},
  title        = {An intrinsic mechanism of corticogenesis from embryonic stem cells},
  url          = {http://dx.doi.org/10.1038/nature07287},
  volume       = {455},
  year         = {2008},
}

Chicago
Gaspard, Nicolas, Tristan Bouschet, Raphael Hourez, Jordane Dimidschstein, Gilles Naeije, Jelle van den Ameele, Ira Espuny-Camacho, et al. 2008. “An Intrinsic Mechanism of Corticogenesis from Embryonic Stem Cells.” Nature 455 (7211): 351–357.
APA
Gaspard, N., Bouschet, T., Hourez, R., Dimidschstein, J., Naeije, G., van den Ameele, J., Espuny-Camacho, I., et al. (2008). An intrinsic mechanism of corticogenesis from embryonic stem cells. NATURE, 455(7211), 351–357.
Vancouver
1.
Gaspard N, Bouschet T, Hourez R, Dimidschstein J, Naeije G, van den Ameele J, et al. An intrinsic mechanism of corticogenesis from embryonic stem cells. NATURE. 2008;455(7211):351–7.
MLA
Gaspard, Nicolas, Tristan Bouschet, Raphael Hourez, et al. “An Intrinsic Mechanism of Corticogenesis from Embryonic Stem Cells.” NATURE 455.7211 (2008): 351–357. Print.