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Generation of cortical neurons from mouse embryonic stem cells

(2009) NATURE PROTOCOLS. 4(10). p.1454-1463
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Abstract
Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains no morphogen, but in the presence of the sonic hedgehog inhibitor cyclopamine. Corticogenesis from ESCs recapitulates the most important steps of cortical development, leading to the generation of multipotent cortical progenitors that sequentially produce cortical pyramidal neurons displaying distinct layer-specific identities. The protocol provides a most reductionist cellular model to tackle the complex mechanisms of cortical development and function, thereby opening new perspectives for the modeling of cortical diseases and the design of novel neurological treatments, while offering an alternative to animal use. In this protocol, we describe a method by which millions of cortical neurons can be generated in 2-3 weeks, starting from a single frozen vial of ESCs.
Keywords
ES CELLS, IN-VITRO DIFFERENTIATION, DIRECTED DIFFERENTIATION, NEURAL DIFFERENTIATION, CEREBRAL-CORTEX, PRECURSORS, INDUCTION, TELENCEPHALON, SPECIFICATION, CONVERSION

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Citation

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Chicago
Gaspard, Nicolas, Tristan Bouschet, Adele Herpoel, Gilles Naeije, Jelle van den Ameele, and Pierre Vanderhaeghen. 2009. “Generation of Cortical Neurons from Mouse Embryonic Stem Cells.” Nature Protocols 4 (10): 1454–1463.
APA
Gaspard, N., Bouschet, T., Herpoel, A., Naeije, G., van den Ameele, J., & Vanderhaeghen, P. (2009). Generation of cortical neurons from mouse embryonic stem cells. NATURE PROTOCOLS, 4(10), 1454–1463.
Vancouver
1.
Gaspard N, Bouschet T, Herpoel A, Naeije G, van den Ameele J, Vanderhaeghen P. Generation of cortical neurons from mouse embryonic stem cells. NATURE PROTOCOLS. 2009;4(10):1454–63.
MLA
Gaspard, Nicolas, Tristan Bouschet, Adele Herpoel, et al. “Generation of Cortical Neurons from Mouse Embryonic Stem Cells.” NATURE PROTOCOLS 4.10 (2009): 1454–1463. Print.
@article{2126014,
  abstract     = {Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains no morphogen, but in the presence of the sonic hedgehog inhibitor cyclopamine. Corticogenesis from ESCs recapitulates the most important steps of cortical development, leading to the generation of multipotent cortical progenitors that sequentially produce cortical pyramidal neurons displaying distinct layer-specific identities. The protocol provides a most reductionist cellular model to tackle the complex mechanisms of cortical development and function, thereby opening new perspectives for the modeling of cortical diseases and the design of novel neurological treatments, while offering an alternative to animal use. In this protocol, we describe a method by which millions of cortical neurons can be generated in 2-3 weeks, starting from a single frozen vial of ESCs.},
  author       = {Gaspard, Nicolas and Bouschet, Tristan and Herpoel, Adele and Naeije, Gilles and van den Ameele, Jelle and Vanderhaeghen, Pierre},
  issn         = {1754-2189},
  journal      = {NATURE PROTOCOLS},
  keyword      = {ES CELLS,IN-VITRO DIFFERENTIATION,DIRECTED DIFFERENTIATION,NEURAL DIFFERENTIATION,CEREBRAL-CORTEX,PRECURSORS,INDUCTION,TELENCEPHALON,SPECIFICATION,CONVERSION},
  language     = {eng},
  number       = {10},
  pages        = {1454--1463},
  title        = {Generation of cortical neurons from mouse embryonic stem cells},
  url          = {http://dx.doi.org/10.1038/nprot.2009.157},
  volume       = {4},
  year         = {2009},
}

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