Ghent University Academic Bibliography

Advanced

Generation of cortical neurons from mouse embryonic stem cells

Nicolas Gaspard, Tristan Bouschet, Adele Herpoel, Gilles Naeije, Jelle van den Ameele UGent and Pierre Vanderhaeghen (2009) NATURE PROTOCOLS. 4(10). p.1454-1463
abstract
Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains no morphogen, but in the presence of the sonic hedgehog inhibitor cyclopamine. Corticogenesis from ESCs recapitulates the most important steps of cortical development, leading to the generation of multipotent cortical progenitors that sequentially produce cortical pyramidal neurons displaying distinct layer-specific identities. The protocol provides a most reductionist cellular model to tackle the complex mechanisms of cortical development and function, thereby opening new perspectives for the modeling of cortical diseases and the design of novel neurological treatments, while offering an alternative to animal use. In this protocol, we describe a method by which millions of cortical neurons can be generated in 2-3 weeks, starting from a single frozen vial of ESCs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ES CELLS, IN-VITRO DIFFERENTIATION, DIRECTED DIFFERENTIATION, NEURAL DIFFERENTIATION, CEREBRAL-CORTEX, PRECURSORS, INDUCTION, TELENCEPHALON, SPECIFICATION, CONVERSION
journal title
NATURE PROTOCOLS
Nat. Protoc.
volume
4
issue
10
pages
1454 - 1463
Web of Science type
Article
Web of Science id
000275367400006
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
6.335 (2009)
JCR rank
6/65 (2009)
JCR quartile
1 (2009)
ISSN
1754-2189
DOI
10.1038/nprot.2009.157
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2126014
handle
http://hdl.handle.net/1854/LU-2126014
date created
2012-05-31 21:47:01
date last changed
2016-12-19 15:42:56
@article{2126014,
  abstract     = {Embryonic stem cells (ESCs) constitute a tool of great potential in neurobiology, enabling the directed differentiation of specific neural cell types. We have shown recently that neurons of the cerebral cortex can be generated from mouse ESCs cultured in a chemically defined medium that contains no morphogen, but in the presence of the sonic hedgehog inhibitor cyclopamine. Corticogenesis from ESCs recapitulates the most important steps of cortical development, leading to the generation of multipotent cortical progenitors that sequentially produce cortical pyramidal neurons displaying distinct layer-specific identities. The protocol provides a most reductionist cellular model to tackle the complex mechanisms of cortical development and function, thereby opening new perspectives for the modeling of cortical diseases and the design of novel neurological treatments, while offering an alternative to animal use. In this protocol, we describe a method by which millions of cortical neurons can be generated in 2-3 weeks, starting from a single frozen vial of ESCs.},
  author       = {Gaspard, Nicolas and Bouschet, Tristan and Herpoel, Adele and Naeije, Gilles and van den Ameele, Jelle and Vanderhaeghen, Pierre},
  issn         = {1754-2189},
  journal      = {NATURE PROTOCOLS},
  keyword      = {ES CELLS,IN-VITRO DIFFERENTIATION,DIRECTED DIFFERENTIATION,NEURAL DIFFERENTIATION,CEREBRAL-CORTEX,PRECURSORS,INDUCTION,TELENCEPHALON,SPECIFICATION,CONVERSION},
  language     = {eng},
  number       = {10},
  pages        = {1454--1463},
  title        = {Generation of cortical neurons from mouse embryonic stem cells},
  url          = {http://dx.doi.org/10.1038/nprot.2009.157},
  volume       = {4},
  year         = {2009},
}

Chicago
Gaspard, Nicolas, Tristan Bouschet, Adele Herpoel, Gilles Naeije, Jelle van den Ameele, and Pierre Vanderhaeghen. 2009. “Generation of Cortical Neurons from Mouse Embryonic Stem Cells.” Nature Protocols 4 (10): 1454–1463.
APA
Gaspard, N., Bouschet, T., Herpoel, A., Naeije, G., van den Ameele, J., & Vanderhaeghen, P. (2009). Generation of cortical neurons from mouse embryonic stem cells. NATURE PROTOCOLS, 4(10), 1454–1463.
Vancouver
1.
Gaspard N, Bouschet T, Herpoel A, Naeije G, van den Ameele J, Vanderhaeghen P. Generation of cortical neurons from mouse embryonic stem cells. NATURE PROTOCOLS. 2009;4(10):1454–63.
MLA
Gaspard, Nicolas, Tristan Bouschet, Adele Herpoel, et al. “Generation of Cortical Neurons from Mouse Embryonic Stem Cells.” NATURE PROTOCOLS 4.10 (2009): 1454–1463. Print.