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Association between CXCR1 gene polymorphisms and udder health in dairy heifers

Joren Verbeke UGent, Sofie Piepers UGent, Luc Peelman UGent, Mario Van Poucke UGent and Sarne De Vliegher UGent (2012) National Mastitis Council 51st Annual Meeting, Abstracts. p.157-158
abstract
Introduction : Mastitis is highly prevalent in periparturient heifers, causing substantial economic losses when major pathogens such as Staphylococcus aureus and Streptococcus uberis are involved (Huijps et al., 2008; Piepers et al., 2010). Effective prevention of heifer mastitis relies on reducing the infection pressure via management as well as on optimizing the host resistance. Part of the variation in host resistance is genetically determined (Nash et al., 2003). Thus, marker-assisted selection against certain disadvantageous genotypes might offer a durable and sustainable preventive measurement against heifer mastitis without the usage of antimicrobials. The main objective of this study was to screen CXCR1 for polymorphisms and to analyze their association with udder health during first lactation. This potential candidate gene encodes the homonymous receptor binding interleukin 8, a crucial chemokine in the innate immunity of the mammary gland (Barber and Yang, 1998). Materials and Methods : In total, 140 heifers originating from 20 Belgian farms were enrolled in the study. Intramammary infection (IMI) status in early lactation at quarter level was determined by bacteriological culture according to NMC procedures. Quarter and composite SCC was measured in early lactation and during lactation at test-day, respectively. Heifers were genotyped for the whole coding region of CXCR1. Sequences were compared to detect polymorphisms. Associations between the detected polymorphisms and quarter IMI status in early lactation, quarter SCC in early lactation and composite SCC during first lactation, respectively, were tested using logistic and linear mixed regression models. Results and Discussion : Fifteen polymorphisms were detected of which 10 have not been described before. The reported association (Galvao et al., 2011; Youngerman et al., 2004) between the polymorphism at position +735 compared to the start codon (c.735C>G) and mastitis resistance could not be confirmed. Instead, the novel polymorphism c.980A>G was found to be associated with pathogen-group specific IMI in early lactation. Heifers expressing genotype AG at this position had significantly less quarters infected with major pathogens compared to heifers expressing genotype GG (P<0.05). Remarkably, these differences were not observed for coagulase-negative staphylococci (CNS) infections (P=0.98). Differences in SCC in early lactation as well as during lactation by CXCR1 genotype were observed, although they were not statistically significant. Conclusion : Results in this study are promising and stimulate to further elucidate the role of CXCR1 in mastitis susceptibility. Selection against certain CXCR1 genotypes might be an interesting tool for prevention of heifer mastitis. References Barber, M.R., and T.J. Yang. 1998. Chemotactic activities in nonmastitic and mastitic mammary secretions: Presence of interleukin-8 in mastitic but not nonmastitic secretions. Clin. Diagn. Lab. Immunol. 5:82-86. Galvao, K.N., G.M. Pighetti, S.H. Cheong, D.V. Nydam, and R.O. Gilbert. 2011. Association between interleukin-8 receptor-alpha (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. J. Dairy Sci. 94:2083-2091. Huijps, K., S. De Vliegher, T. Lam, and H. Hogeveen. 2008. Cost estimation of heifer mastitis in early lactation by stochastic modelling. Vet Microbiol. 134:121-127. Nash, D.L., G.W. Rogers, J.B. Cooper, G.L. Hargrove, and J.F. Keown. 2003. Heritability of intramammary infections at first parturition and relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. J Dairy Sci. 86:2684-2695. Piepers, S., G. Opsomer, H. W. Barkema, A. de Kruif, and S. De Vliegher. 2010. Heifers infected with coagulase-negative staphylococci in early lactation have fewer cases of clinical mastitis and higher milk production in their first lactation than noninfected heifers. J. Dairy Sci. 93:2014-2024. Youngerman, S.M., A.M. Saxton, S.P. Oliver, and G.M. Pighetti. 2004. Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci. 87:2442-2448.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
National Mastitis Council 51st Annual Meeting, Abstracts
pages
157 - 158
publisher
National Mastitis Council (NMC)
conference name
National Mastitis Council 51st Annual Meeting
conference location
St Pete Beach, FL, USA
conference start
2012-01-22
conference end
2012-01-24
language
English
UGent publication?
yes
classification
C3
id
2125834
handle
http://hdl.handle.net/1854/LU-2125834
date created
2012-05-31 17:14:14
date last changed
2012-06-12 16:08:14
@inproceedings{2125834,
  abstract     = {Introduction : Mastitis is highly prevalent in periparturient heifers, causing substantial economic losses when major pathogens such as Staphylococcus aureus and Streptococcus uberis are involved (Huijps et al., 2008; Piepers et al., 2010). Effective prevention of heifer mastitis relies on reducing the infection pressure via management as well as on optimizing the host resistance. Part of the variation in host resistance is genetically determined (Nash et al., 2003). Thus, marker-assisted selection against certain disadvantageous genotypes might offer a durable and sustainable preventive measurement against heifer mastitis without the usage of antimicrobials. The main objective of this study was to screen CXCR1 for polymorphisms and to analyze their association with udder health during first lactation. This potential candidate gene encodes the homonymous receptor binding interleukin 8, a crucial chemokine in the innate immunity of the mammary gland (Barber and Yang, 1998).
Materials and Methods : In total, 140 heifers originating from 20 Belgian farms were enrolled in the study. Intramammary infection (IMI) status in early lactation at quarter level was determined by bacteriological culture according to NMC procedures. Quarter and composite SCC was measured in early lactation and during lactation at test-day, respectively. Heifers were genotyped for the whole coding region of CXCR1. Sequences were compared to detect polymorphisms. Associations between the detected polymorphisms and quarter IMI status in early lactation, quarter SCC in early lactation and composite SCC during first lactation, respectively, were tested using logistic and linear mixed regression models.
Results and Discussion : Fifteen polymorphisms were detected of which 10 have not been described before. The reported association (Galvao et al., 2011; Youngerman et al., 2004) between the polymorphism at position +735 compared to the start codon (c.735C{\textrangle}G) and mastitis resistance could not be confirmed. Instead, the novel polymorphism c.980A{\textrangle}G was found to be associated with pathogen-group specific IMI in early lactation. Heifers expressing genotype AG at this position had significantly less quarters infected with major pathogens compared to heifers expressing genotype GG (P{\textlangle}0.05). Remarkably, these differences were not observed for coagulase-negative staphylococci (CNS) infections (P=0.98). Differences in SCC in early lactation as well as during lactation by CXCR1 genotype were observed, although they were not statistically significant.
Conclusion : Results in this study are promising and stimulate to further elucidate the role of CXCR1 in mastitis susceptibility. Selection against certain CXCR1 genotypes might be an interesting tool for prevention of heifer mastitis.
References
Barber, M.R., and T.J. Yang. 1998. Chemotactic activities in nonmastitic and mastitic mammary secretions: Presence of interleukin-8 in mastitic but not nonmastitic secretions. Clin. Diagn. Lab. Immunol. 5:82-86.
Galvao, K.N., G.M. Pighetti, S.H. Cheong, D.V. Nydam, and R.O. Gilbert. 2011. Association between interleukin-8 receptor-alpha (CXCR1) polymorphism and disease incidence, production, reproduction, and survival in Holstein cows. J. Dairy Sci. 94:2083-2091.
Huijps, K., S. De Vliegher, T. Lam, and H. Hogeveen. 2008. Cost estimation of heifer mastitis in early lactation by stochastic modelling. Vet Microbiol. 134:121-127.
Nash, D.L., G.W. Rogers, J.B. Cooper, G.L. Hargrove, and J.F. Keown. 2003. Heritability of intramammary infections at first parturition and relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. J Dairy Sci. 86:2684-2695.
Piepers, S., G. Opsomer, H. W. Barkema, A. de Kruif, and S. De Vliegher. 2010. Heifers infected with coagulase-negative staphylococci in early lactation have fewer cases of clinical mastitis and higher milk production in their first lactation than noninfected heifers. J. Dairy Sci. 93:2014-2024.
Youngerman, S.M., A.M. Saxton, S.P. Oliver, and G.M. Pighetti. 2004. Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci. 87:2442-2448.},
  author       = {Verbeke, Joren and Piepers, Sofie and Peelman, Luc and Van Poucke, Mario and De Vliegher, Sarne},
  booktitle    = {National Mastitis Council 51st Annual Meeting, Abstracts},
  language     = {eng},
  location     = {St Pete Beach, FL, USA},
  pages        = {157--158},
  publisher    = {National Mastitis Council (NMC)},
  title        = {Association between CXCR1 gene polymorphisms and udder health in dairy heifers},
  year         = {2012},
}

Chicago
Verbeke, Joren, Sofie Piepers, Luc Peelman, Mario Van Poucke, and Sarne De Vliegher. 2012. “Association Between CXCR1 Gene Polymorphisms and Udder Health in Dairy Heifers.” In National Mastitis Council 51st Annual Meeting, Abstracts, 157–158. National Mastitis Council (NMC).
APA
Verbeke, J., Piepers, S., Peelman, L., Van Poucke, M., & De Vliegher, S. (2012). Association between CXCR1 gene polymorphisms and udder health in dairy heifers. National Mastitis Council 51st Annual Meeting, Abstracts (pp. 157–158). Presented at the National Mastitis Council 51st Annual Meeting, National Mastitis Council (NMC).
Vancouver
1.
Verbeke J, Piepers S, Peelman L, Van Poucke M, De Vliegher S. Association between CXCR1 gene polymorphisms and udder health in dairy heifers. National Mastitis Council 51st Annual Meeting, Abstracts. National Mastitis Council (NMC); 2012. p. 157–8.
MLA
Verbeke, Joren, Sofie Piepers, Luc Peelman, et al. “Association Between CXCR1 Gene Polymorphisms and Udder Health in Dairy Heifers.” National Mastitis Council 51st Annual Meeting, Abstracts. National Mastitis Council (NMC), 2012. 157–158. Print.