Ghent University Academic Bibliography

Advanced

The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling

Jan Smalle UGent, Jasmina Kurepa UGent, Peizhen Yang, Thomas J Emborg, Elena Babiychuk UGent, Sergei Kushnir UGent and Richard D Vierstra (2003) PLANT CELL. 15(4). p.965-980
abstract
The 26S proteasome is an essential protease complex responsible for removing most short-lived intracellular proteins, especially those modified with polyubiquitin chains. We show here that an Arabidopsis mutant expressing an altered RPN10 subunit exhibited a pleiotropic phenotype consistent with specific changes in 26S proteasome function. rpn10-1 plants displayed reduced seed germination, growth rate, stamen number, genetic transmission through the male gamete, and hormone-induced cell division, which can be explained partially by a constitutive downregulation of the key cell cycle gene CDKA;I. rpn10-1 also was more sensitive to abscisic acid (ABA), salt, and sucrose stress and to DNA-damaging agents and had decreased sensitivity to cytokinin and auxin. Most of the phenotypes can be explained by a hypersensitivity to ABA, which is reflected at the molecular level by the selective stabilization of the short-lived ABA-signaling protein AB15. Collectively, these results indicate that RPN10 affects a number of regulatory processes in Arabidopsis likely by directing specific proteins to the 26S proteasome for degradation. A particularly important role may be in regulating the responses to signals promulgated by ABA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
S PROTEASOME, KINASE-ACTIVITY, TRANSCRIPTION FACTOR, SACCHAROMYCES-CEREVISIAE, CELL-DIVISION, REGULATORY PARTICLE, PROTEIN-DEGRADATION, NON-ATPASE SUBUNIT, MULTIUBIQUITIN-CHAIN-BINDING, UBIQUITIN-DEPENDENT PROTEOLYSIS
journal title
PLANT CELL
Plant Cell
volume
15
issue
4
pages
965 - 980
Web of Science type
Article
Web of Science id
000185078100015
JCR category
PLANT SCIENCES
JCR impact factor
10.679 (2003)
JCR rank
3/136 (2003)
JCR quartile
1 (2003)
ISSN
1040-4651
DOI
10.1105/tpc.009217
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
212374
handle
http://hdl.handle.net/1854/LU-212374
date created
2004-04-22 18:16:00
date last changed
2013-10-11 15:52:18
@article{212374,
  abstract     = {The 26S proteasome is an essential protease complex responsible for removing most short-lived intracellular proteins, especially those modified with polyubiquitin chains. We show here that an Arabidopsis mutant expressing an altered RPN10 subunit exhibited a pleiotropic phenotype consistent with specific changes in 26S proteasome function. rpn10-1 plants displayed reduced seed germination, growth rate, stamen number, genetic transmission through the male gamete, and hormone-induced cell division, which can be explained partially by a constitutive downregulation of the key cell cycle gene CDKA;I. rpn10-1 also was more sensitive to abscisic acid (ABA), salt, and sucrose stress and to DNA-damaging agents and had decreased sensitivity to cytokinin and auxin. Most of the phenotypes can be explained by a hypersensitivity to ABA, which is reflected at the molecular level by the selective stabilization of the short-lived ABA-signaling protein AB15. Collectively, these results indicate that RPN10 affects a number of regulatory processes in Arabidopsis likely by directing specific proteins to the 26S proteasome for degradation. A particularly important role may be in regulating the responses to signals promulgated by ABA.},
  author       = {Smalle, Jan and Kurepa, Jasmina and Yang, Peizhen and Emborg, Thomas J and Babiychuk, Elena and Kushnir, Sergei and Vierstra, Richard D},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {S PROTEASOME,KINASE-ACTIVITY,TRANSCRIPTION FACTOR,SACCHAROMYCES-CEREVISIAE,CELL-DIVISION,REGULATORY PARTICLE,PROTEIN-DEGRADATION,NON-ATPASE SUBUNIT,MULTIUBIQUITIN-CHAIN-BINDING,UBIQUITIN-DEPENDENT PROTEOLYSIS},
  language     = {eng},
  number       = {4},
  pages        = {965--980},
  title        = {The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling},
  url          = {http://dx.doi.org/10.1105/tpc.009217},
  volume       = {15},
  year         = {2003},
}

Chicago
Smalle, Jan, Jasmina Kurepa, Peizhen Yang, Thomas J Emborg, Elena Babiychuk, Sergei Kushnir, and Richard D Vierstra. 2003. “The Pleiotropic Role of the 26S Proteasome Subunit RPN10 in Arabidopsis Growth and Development Supports a Substrate-specific Function in Abscisic Acid Signaling.” Plant Cell 15 (4): 965–980.
APA
Smalle, J., Kurepa, J., Yang, P., Emborg, T. J., Babiychuk, E., Kushnir, S., & Vierstra, R. D. (2003). The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. PLANT CELL, 15(4), 965–980.
Vancouver
1.
Smalle J, Kurepa J, Yang P, Emborg TJ, Babiychuk E, Kushnir S, et al. The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. PLANT CELL. 2003;15(4):965–80.
MLA
Smalle, Jan, Jasmina Kurepa, Peizhen Yang, et al. “The Pleiotropic Role of the 26S Proteasome Subunit RPN10 in Arabidopsis Growth and Development Supports a Substrate-specific Function in Abscisic Acid Signaling.” PLANT CELL 15.4 (2003): 965–980. Print.