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Physiological and morphological evidence of brassinosteroid-biosynthesis inhibition by the fungicide imazalil

(2003) PHYSIOLOGIA PLANTARUM. 119(1). p.69-77
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Abstract
Dark germinated Arabidopsis thaliana Ler seedlings grown on medium with the commonly used imidazole-type fungicide imazalil (IMA) resemble de-etiolated (det) brassinosteroid-deficient mutants. IMA hampers cell elongation in the hypocotyl, but stimulates radial expansion during dark growth. This phenotype could partially be restored by simultaneous addition of 24-epibrassinolide (EBR). A complete restoration of the hypocotyl length could only be achieved by combining EBR and gibberellic acid. As Arabidopsis thaliana etr1-3 de-etiolates on IMA containing medium in the dark, in the same dose-dependent manner as the wild type, its effects cannot be attributed to the induction of extra ethylene production. Studies with A. thaliana seedlings expressing CPD::GUS show that IMA up-regulates the expression of CPD, which encodes a key cytochrome P450 enzyme in the brassinosteroid (BR)-biosynthesis pathway. This effect is reverted by EBR, indicating that the up-regulation of CPD by IMA might result from the lack of end product brassinolide. Together these data suggest that, in Arabidopsis, one of the effects of IMA is an inhibition of BR-biosynthesis. IMA is an available and cheap agrochemical that might be a valuable tool for future brassinosteroid research.
Keywords
GIBBERELLINS, GENE, PACLOBUTRAZOL, CELL ELONGATION, CYTOCHROME-P450, ETHYLENE, ARABIDOPSIS-THALIANA, IMIDAZOLE FUNGICIDES, TRACHEARY-ELEMENT DIFFERENTIATION, GROWTH

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Chicago
Werbrouck, Stefaan, Nelson JM Saibo, Hilde Dhuyvetter, Sandra De Schepper, Dominique Van Der Straeten, and Pierre Debergh. 2003. “Physiological and Morphological Evidence of Brassinosteroid-biosynthesis Inhibition by the Fungicide Imazalil.” Physiologia Plantarum 119 (1): 69–77.
APA
Werbrouck, Stefaan, Saibo, N. J., Dhuyvetter, H., De Schepper, S., Van Der Straeten, D., & Debergh, P. (2003). Physiological and morphological evidence of brassinosteroid-biosynthesis inhibition by the fungicide imazalil. PHYSIOLOGIA PLANTARUM, 119(1), 69–77.
Vancouver
1.
Werbrouck S, Saibo NJ, Dhuyvetter H, De Schepper S, Van Der Straeten D, Debergh P. Physiological and morphological evidence of brassinosteroid-biosynthesis inhibition by the fungicide imazalil. PHYSIOLOGIA PLANTARUM. 2003;119(1):69–77.
MLA
Werbrouck, Stefaan, Nelson JM Saibo, Hilde Dhuyvetter, et al. “Physiological and Morphological Evidence of Brassinosteroid-biosynthesis Inhibition by the Fungicide Imazalil.” PHYSIOLOGIA PLANTARUM 119.1 (2003): 69–77. Print.
@article{212212,
  abstract     = {Dark germinated Arabidopsis thaliana Ler seedlings grown on medium with the commonly used imidazole-type fungicide imazalil (IMA) resemble de-etiolated (det) brassinosteroid-deficient mutants. IMA hampers cell elongation in the hypocotyl, but stimulates radial expansion during dark growth. This phenotype could partially be restored by simultaneous addition of 24-epibrassinolide (EBR). A complete restoration of the hypocotyl length could only be achieved by combining EBR and gibberellic acid. As Arabidopsis thaliana etr1-3 de-etiolates on IMA containing medium in the dark, in the same dose-dependent manner as the wild type, its effects cannot be attributed to the induction of extra ethylene production. Studies with A. thaliana seedlings expressing CPD::GUS show that IMA up-regulates the expression of CPD, which encodes a key cytochrome P450 enzyme in the brassinosteroid (BR)-biosynthesis pathway. This effect is reverted by EBR, indicating that the up-regulation of CPD by IMA might result from the lack of end product brassinolide. Together these data suggest that, in Arabidopsis, one of the effects of IMA is an inhibition of BR-biosynthesis. IMA is an available and cheap agrochemical that might be a valuable tool for future brassinosteroid research.},
  author       = {Werbrouck, Stefaan and Saibo, Nelson JM and Dhuyvetter, Hilde and De Schepper, Sandra and Van Der Straeten, Dominique and Debergh, Pierre},
  issn         = {0031-9317},
  journal      = {PHYSIOLOGIA PLANTARUM},
  keyword      = {GIBBERELLINS,GENE,PACLOBUTRAZOL,CELL ELONGATION,CYTOCHROME-P450,ETHYLENE,ARABIDOPSIS-THALIANA,IMIDAZOLE FUNGICIDES,TRACHEARY-ELEMENT DIFFERENTIATION,GROWTH},
  language     = {eng},
  number       = {1},
  pages        = {69--77},
  title        = {Physiological and morphological evidence of brassinosteroid-biosynthesis inhibition by the fungicide imazalil},
  url          = {http://dx.doi.org/10.1034/j.1399-3054.2003.00155.x},
  volume       = {119},
  year         = {2003},
}

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