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Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots

Maricel Alvarez, Dries Huygens UGent, Carlos Fernandez, Yessy Gacitua, Erick Olivares, Isabel Saavedra, Miren Alberdi and Eduardo Valenzuela (2009) TREE PHYSIOLOGY. 29(8). p.1047-1057
abstract
Infection with ectomycorrhizal fungi can increase the ability of plants to resist drought stress through morphophysiological and biochemical mechanisms. However, the metabolism of antioxidative enzyme activities in the ectomycorrhizal symbiosis remains poorly understood. This study investigated biomass production, reactive oxygen metabolism (hydrogen peroxide and malondialdehyde concentration) and antioxidant enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in pure cultures of the ectomycorrhizal fungi Descolea antartica Sing. and Pisolithus tinctorius (Pers.) Coker & Couch, and non-mycorrhizal and mycorrhizal roots of Nothofagus dombeyi (Mirb.) roots under well-watered conditions and drought conditions (DC). The studied ectomycorrhizal fungi regulated their antioxidative enzyme metabolism differentially in response to drought, resulting in cellular damage in D. antartica but not in P. tinctorius. Ectomycorrhizal inoculation and water treatment had a significant effect on all parameters studied, including relative water content of the plant. As such, N. dombeyi plants in symbiosis experienced a lower oxidative stress effect than non-mycorrhizal plants under DC. Additionally, ectomycorrhizal N. dombeyi roots showed a greater antioxidant enzyme activity relative to non-mycorrhizal roots, an effect which was further expressed under DC. The association between the non-specific P. tinctorius and N. dombeyi had a more effective reactive oxygen species (ROS) metabolism than the specific D. antartica-N. dombeyi symbiosis. We conclude that the combination of effective ROS prevention and ROS detoxification by ectomycorrhizal plants resulted in reduced cellular damage and increased plant growth relative to non-mycorrhizal plants under drought.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SOYBEAN PLANTS, NITRIC-OXIDE, NODULE SENESCENCE, ANTIOXIDANT SYSTEMS, LYCOPERSICON-PENNELLII, HYDROGEN-PEROXIDE, OXIDATIVE STRESS, WATER-STRESS, ASCORBATE-GLUTATHIONE CYCLE, SUPEROXIDE-DISMUTASE ACTIVITY, volcanic soil, superoxide dismutase, pristine forest, glutathione reductase, ascorbate peroxidase, catalase
journal title
TREE PHYSIOLOGY
Tree Physiol.
volume
29
issue
8
pages
1047 - 1057
Web of Science type
Article
Web of Science id
000268117200008
JCR category
FORESTRY
JCR impact factor
2.292 (2009)
JCR rank
3/46 (2009)
JCR quartile
1 (2009)
ISSN
0829-318X
DOI
10.1093/treephys/tpp038
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2120390
handle
http://hdl.handle.net/1854/LU-2120390
date created
2012-05-30 10:07:07
date last changed
2016-12-19 15:42:56
@article{2120390,
  abstract     = {Infection with ectomycorrhizal fungi can increase the ability of plants to resist drought stress through morphophysiological and biochemical mechanisms. However, the metabolism of antioxidative enzyme activities in the ectomycorrhizal symbiosis remains poorly understood. This study investigated biomass production, reactive oxygen metabolism (hydrogen peroxide and malondialdehyde concentration) and antioxidant enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in pure cultures of the ectomycorrhizal fungi Descolea antartica Sing. and Pisolithus tinctorius (Pers.) Coker \& Couch, and non-mycorrhizal and mycorrhizal roots of Nothofagus dombeyi (Mirb.) roots under well-watered conditions and drought conditions (DC). The studied ectomycorrhizal fungi regulated their antioxidative enzyme metabolism differentially in response to drought, resulting in cellular damage in D. antartica but not in P. tinctorius. Ectomycorrhizal inoculation and water treatment had a significant effect on all parameters studied, including relative water content of the plant. As such, N. dombeyi plants in symbiosis experienced a lower oxidative stress effect than non-mycorrhizal plants under DC. Additionally, ectomycorrhizal N. dombeyi roots showed a greater antioxidant enzyme activity relative to non-mycorrhizal roots, an effect which was further expressed under DC. The association between the non-specific P. tinctorius and N. dombeyi had a more effective reactive oxygen species (ROS) metabolism than the specific D. antartica-N. dombeyi symbiosis. We conclude that the combination of effective ROS prevention and ROS detoxification by ectomycorrhizal plants resulted in reduced cellular damage and increased plant growth relative to non-mycorrhizal plants under drought.},
  author       = {Alvarez, Maricel and Huygens, Dries and Fernandez, Carlos and Gacitua, Yessy and Olivares, Erick and Saavedra, Isabel and Alberdi, Miren and Valenzuela, Eduardo},
  issn         = {0829-318X},
  journal      = {TREE PHYSIOLOGY},
  keyword      = {SOYBEAN PLANTS,NITRIC-OXIDE,NODULE SENESCENCE,ANTIOXIDANT SYSTEMS,LYCOPERSICON-PENNELLII,HYDROGEN-PEROXIDE,OXIDATIVE STRESS,WATER-STRESS,ASCORBATE-GLUTATHIONE CYCLE,SUPEROXIDE-DISMUTASE ACTIVITY,volcanic soil,superoxide dismutase,pristine forest,glutathione reductase,ascorbate peroxidase,catalase},
  language     = {eng},
  number       = {8},
  pages        = {1047--1057},
  title        = {Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots},
  url          = {http://dx.doi.org/10.1093/treephys/tpp038},
  volume       = {29},
  year         = {2009},
}

Chicago
Alvarez, Maricel, Dries Huygens, Carlos Fernandez, Yessy Gacitua, Erick Olivares, Isabel Saavedra, Miren Alberdi, and Eduardo Valenzuela. 2009. “Effect of Ectomycorrhizal Colonization and Drought on Reactive Oxygen Species Metabolism of Nothofagus Dombeyi Roots.” Tree Physiology 29 (8): 1047–1057.
APA
Alvarez, M., Huygens, D., Fernandez, C., Gacitua, Y., Olivares, E., Saavedra, I., Alberdi, M., et al. (2009). Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots. TREE PHYSIOLOGY, 29(8), 1047–1057.
Vancouver
1.
Alvarez M, Huygens D, Fernandez C, Gacitua Y, Olivares E, Saavedra I, et al. Effect of ectomycorrhizal colonization and drought on reactive oxygen species metabolism of Nothofagus dombeyi roots. TREE PHYSIOLOGY. 2009;29(8):1047–57.
MLA
Alvarez, Maricel, Dries Huygens, Carlos Fernandez, et al. “Effect of Ectomycorrhizal Colonization and Drought on Reactive Oxygen Species Metabolism of Nothofagus Dombeyi Roots.” TREE PHYSIOLOGY 29.8 (2009): 1047–1057. Print.