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The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake

Maricel Alvarez, Dries Huygens UGent, Leila Milena Diaz, Claudia Anazco Villanueva, Wolfgang Heyser and Pascal Boeckx UGent (2012) PLANT CELL AND ENVIRONMENT. 35(1). p.126-135
abstract
Acid phosphatase (ACP) enzymes are involved in the mobilization of soil phosphorus (P) and polyphosphate accumulated in the fungal tissues of ectomycorrhizal roots, thereby influencing the amounts of P that are stored in the fungus and transferred to the host plant. This study evaluated the effects of ectomycorrhizal morphotype and soil fertility on ACP activity in the extraradical mycelium (ACPmyc), the mantle (ACPmantle) and the Hartig net region (ACPHartig) of ectomycorrhizal Nothofagus obliqua seedlings. ACP activity was quantified in vivo using enzyme-labelled fluorescence-97 (ELF-97) substrate, confocal laser microscopy and digital image processing routines. There was a significant effect of ectomycorrhizal morphotype on ACPmyc, ACPmantle and ACPHartig, while soil fertility had a significant effect on ACPmyc and ACPHartig. The relative contribution of the mantle and the Hartig net region to the ACP activity on the ectomycorrhizal root was significantly affected by ectomycorrhizal morphotype and soil fertility. A positive correlation between ACPHartig and the shoot P concentration was found, providing evidence that ACP activity at the fungus:root interface is involved in P transfer from the fungus to the host. It is concluded that the spatial distribution of ACP in ectomycorrhizas varies as a function of soil fertility and colonizing fungus.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXPLORATION TYPES, MYCORRHIZAL FUNGI, NOTHOFAGUS-OBLIQUA, NONMYCORRHIZAL ROOTS, enzyme-labelled fluorescence (ELF-97), ectomycorrhiza, digital image processing, Nothofagus obliqua, confocal laser scanning microscopy, SUILLUS BOVINUS, SEEDLINGS, PINE, HEBELOMA, ACQUISITION, NITROGEN
journal title
PLANT CELL AND ENVIRONMENT
Plant Cell Environ.
volume
35
issue
1
pages
126 - 135
Web of Science type
Article
Web of Science id
000298260400010
JCR category
PLANT SCIENCES
JCR impact factor
5.135 (2012)
JCR rank
14/193 (2012)
JCR quartile
1 (2012)
ISSN
0140-7791
DOI
10.1111/j.1365-3040.2011.02422.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2120374
handle
http://hdl.handle.net/1854/LU-2120374
date created
2012-05-30 10:07:07
date last changed
2014-05-26 10:03:18
@article{2120374,
  abstract     = {Acid phosphatase (ACP) enzymes are involved in the mobilization of soil phosphorus (P) and polyphosphate accumulated in the fungal tissues of ectomycorrhizal roots, thereby influencing the amounts of P that are stored in the fungus and transferred to the host plant. This study evaluated the effects of ectomycorrhizal morphotype and soil fertility on ACP activity in the extraradical mycelium (ACPmyc), the mantle (ACPmantle) and the Hartig net region (ACPHartig) of ectomycorrhizal Nothofagus obliqua seedlings. ACP activity was quantified in vivo using enzyme-labelled fluorescence-97 (ELF-97) substrate, confocal laser microscopy and digital image processing routines. There was a significant effect of ectomycorrhizal morphotype on ACPmyc, ACPmantle and ACPHartig, while soil fertility had a significant effect on ACPmyc and ACPHartig. The relative contribution of the mantle and the Hartig net region to the ACP activity on the ectomycorrhizal root was significantly affected by ectomycorrhizal morphotype and soil fertility. A positive correlation between ACPHartig and the shoot P concentration was found, providing evidence that ACP activity at the fungus:root interface is involved in P transfer from the fungus to the host. It is concluded that the spatial distribution of ACP in ectomycorrhizas varies as a function of soil fertility and colonizing fungus.},
  author       = {Alvarez, Maricel and Huygens, Dries and Diaz, Leila Milena and Villanueva, Claudia Anazco and Heyser, Wolfgang and Boeckx, Pascal},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keyword      = {EXPLORATION TYPES,MYCORRHIZAL FUNGI,NOTHOFAGUS-OBLIQUA,NONMYCORRHIZAL ROOTS,enzyme-labelled fluorescence (ELF-97),ectomycorrhiza,digital image processing,Nothofagus obliqua,confocal laser scanning microscopy,SUILLUS BOVINUS,SEEDLINGS,PINE,HEBELOMA,ACQUISITION,NITROGEN},
  language     = {eng},
  number       = {1},
  pages        = {126--135},
  title        = {The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake},
  url          = {http://dx.doi.org/10.1111/j.1365-3040.2011.02422.x},
  volume       = {35},
  year         = {2012},
}

Chicago
Alvarez, Maricel, Dries Huygens, Leila Milena Diaz, Claudia Anazco Villanueva, Wolfgang Heyser, and Pascal Boeckx. 2012. “The Spatial Distribution of Acid Phosphatase Activity in Ectomycorrhizal Tissues Depends on Soil Fertility and Morphotype, and Relates to Host Plant Phosphorus Uptake.” Plant Cell and Environment 35 (1): 126–135.
APA
Alvarez, M., Huygens, D., Diaz, L. M., Villanueva, C. A., Heyser, W., & Boeckx, P. (2012). The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake. PLANT CELL AND ENVIRONMENT, 35(1), 126–135.
Vancouver
1.
Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P. The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake. PLANT CELL AND ENVIRONMENT. 2012;35(1):126–35.
MLA
Alvarez, Maricel, Dries Huygens, Leila Milena Diaz, et al. “The Spatial Distribution of Acid Phosphatase Activity in Ectomycorrhizal Tissues Depends on Soil Fertility and Morphotype, and Relates to Host Plant Phosphorus Uptake.” PLANT CELL AND ENVIRONMENT 35.1 (2012): 126–135. Print.