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A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco

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Abstract
Hydrogen peroxide plays a central role in launching the defense response during stress in plants. To establish a molecular profile provoked by a sustained increase in hydrogen peroxide levels, catalase-deficient tobacco plants (CAT1AS) were exposed to high light (HL) intensities over a detailed time course. The expression kinetics of >14,000 genes were monitored by using transcript profiling technology based on cDNA-amplified fragment length polymorphism. Clustering and sequence analysis of 713 differentially expressed transcript fragments revealed a transcriptional response that mimicked that reported during both biotic and abiotic stresses, including the up-regulation of genes involved in the hypersensitive response, vesicular transport, posttranscriptional processes, biosynthesis of ethylene and jasmonic acid, proteolysis, mitochondrial metabolism, and cell death, and was accompanied by a very rapid up-regulation of several signal transduction components. Expression profiling corroborated by functional experiments showed that HL induced photoinhibition in CAT1AS plants and that a short-term HL exposure of CAT1AS plants triggered an increased tolerance against a subsequent severe oxidative stress.
Keywords
PROTEIN, ARABIDOPSIS, NITRIC-OXIDE, RECEPTOR KINASE, PLANTS, MEMBRANE, SACCHAROMYCES-CEREVISIAE, PROGRAMMED CELL-DEATH, OXIDATIVE STRESS, GENOME-WIDE EXPRESSION

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Chicago
Vandenabeele, Steven, Katrien Van Der Kelen, James F Dat, Ilya Gadjev, Tom Boonefaes, Stijn Morsa, Pieter Rottiers, et al. 2003. “A Comprehensive Analysis of Hydrogen Peroxide-induced Gene Expression in Tobacco.” Proceedings of the National Academy of Sciences of the United States of America 100 (26): 16113–16118.
APA
Vandenabeele, Steven, Van Der Kelen, K., Dat, J. F., Gadjev, I., Boonefaes, T., Morsa, S., Rottiers, P., et al. (2003). A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100(26), 16113–16118.
Vancouver
1.
Vandenabeele S, Van Der Kelen K, Dat JF, Gadjev I, Boonefaes T, Morsa S, et al. A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2003;100(26):16113–8.
MLA
Vandenabeele, Steven, Katrien Van Der Kelen, James F Dat, et al. “A Comprehensive Analysis of Hydrogen Peroxide-induced Gene Expression in Tobacco.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 100.26 (2003): 16113–16118. Print.
@article{211967,
  abstract     = {Hydrogen peroxide plays a central role in launching the defense response during stress in plants. To establish a molecular profile provoked by a sustained increase in hydrogen peroxide levels, catalase-deficient tobacco plants (CAT1AS) were exposed to high light (HL) intensities over a detailed time course. The expression kinetics of {\textrangle}14,000 genes were monitored by using transcript profiling technology based on cDNA-amplified fragment length polymorphism. Clustering and sequence analysis of 713 differentially expressed transcript fragments revealed a transcriptional response that mimicked that reported during both biotic and abiotic stresses, including the up-regulation of genes involved in the hypersensitive response, vesicular transport, posttranscriptional processes, biosynthesis of ethylene and jasmonic acid, proteolysis, mitochondrial metabolism, and cell death, and was accompanied by a very rapid up-regulation of several signal transduction components. Expression profiling corroborated by functional experiments showed that HL induced photoinhibition in CAT1AS plants and that a short-term HL exposure of CAT1AS plants triggered an increased tolerance against a subsequent severe oxidative stress.},
  author       = {Vandenabeele, Steven and Van Der Kelen, Katrien and Dat, James F and Gadjev, Ilya and Boonefaes, Tom and Morsa, Stijn and Rottiers, Pieter and Slooten, Luit and Van Montagu, Marc and Zabeau, Marc and Inz{\'e}, Dirk and Van Breusegem, Frank},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {PROTEIN,ARABIDOPSIS,NITRIC-OXIDE,RECEPTOR KINASE,PLANTS,MEMBRANE,SACCHAROMYCES-CEREVISIAE,PROGRAMMED CELL-DEATH,OXIDATIVE STRESS,GENOME-WIDE EXPRESSION},
  language     = {eng},
  number       = {26},
  pages        = {16113--16118},
  title        = {A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco},
  url          = {http://dx.doi.org/10.1073/pnas.2136610100},
  volume       = {100},
  year         = {2003},
}

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