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Preparation and use of sucrose phosphorylase as cross-linked enzyme aggregate (CLEA)

Karel De Winter (UGent) , Tom Desmet (UGent) , An Cerdobbel (UGent) and Wim Soetaert (UGent)
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Abstract
Sucrose phosphorylase (SP) can be used for the production of α-D-glucose-1-phosphate (α-G1P) by the phosphorolysis of sucrose into α-G1P and fructose. Moreover, a variety of glycosylated compounds can be produced with this enzyme, thanks to its broad acceptor specificity. Unfortunately, the use of SP at industrial scale is hampered by the lack of thermostable enzyme variants. We have recently found, however, that the stability of the SP from Bifidobacterium adolescentis can be dramatically improved by immobilization, either by covalent coupling to a Sepabeads carrier or by forming cross-linked enzyme aggregates (CLEAs).
Keywords
Sucrose phosphorylase, α-D-glucose-1-phosphate, Production, CLEAs

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Chicago
De Winter, Karel, Tom Desmet, An Cerdobbel, and Wim Soetaert. 2012. “Preparation and Use of Sucrose Phosphorylase as Cross-linked Enzyme Aggregate (CLEA).” In Practical Methods for Biocatalysis and Biotransformations 2, ed. John Whittall and Peter W Sutton, 240–244. Chichester, UK: Wiley.
APA
De Winter, K., Desmet, T., Cerdobbel, A., & Soetaert, W. (2012). Preparation and use of sucrose phosphorylase as cross-linked enzyme aggregate (CLEA). In J. Whittall & P. W. Sutton (Eds.), Practical methods for biocatalysis and biotransformations 2 (pp. 240–244). Chichester, UK: Wiley.
Vancouver
1.
De Winter K, Desmet T, Cerdobbel A, Soetaert W. Preparation and use of sucrose phosphorylase as cross-linked enzyme aggregate (CLEA). In: Whittall J, Sutton PW, editors. Practical methods for biocatalysis and biotransformations 2. Chichester, UK: Wiley; 2012. p. 240–4.
MLA
De Winter, Karel, Tom Desmet, An Cerdobbel, et al. “Preparation and Use of Sucrose Phosphorylase as Cross-linked Enzyme Aggregate (CLEA).” Practical Methods for Biocatalysis and Biotransformations 2. Ed. John Whittall & Peter W Sutton. Chichester, UK: Wiley, 2012. 240–244. Print.
@incollection{2119158,
  abstract     = {Sucrose phosphorylase (SP) can be used for the production of \ensuremath{\alpha}-D-glucose-1-phosphate (\ensuremath{\alpha}-G1P) by the phosphorolysis of sucrose into \ensuremath{\alpha}-G1P and fructose. Moreover, a variety of glycosylated compounds can be produced with this enzyme, thanks to its broad acceptor specificity. Unfortunately, the use of SP at industrial scale is hampered by the lack of thermostable enzyme variants. We have recently found, however, that the stability of the SP from Bifidobacterium adolescentis can be dramatically improved by immobilization, either by covalent coupling to a Sepabeads carrier or by forming cross-linked enzyme aggregates (CLEAs).},
  author       = {De Winter, Karel and Desmet, Tom and Cerdobbel, An and Soetaert, Wim},
  booktitle    = {Practical methods for biocatalysis and biotransformations 2},
  editor       = {Whittall, John and Sutton, Peter W},
  isbn         = {9781119943426},
  keyword      = {Sucrose phosphorylase,\ensuremath{\alpha}-D-glucose-1-phosphate,Production,CLEAs},
  language     = {eng},
  pages        = {240--244},
  publisher    = {Wiley},
  title        = {Preparation and use of sucrose phosphorylase as cross-linked enzyme aggregate (CLEA)},
  url          = {http://dx.doi.org/10.1002/9781119943426.ch9},
  year         = {2012},
}

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