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Development and validation of an LC-MS/MS method for the simultaneous determination of deoxynivalenol, zearalenone, T-2 toxin and some masked metabolites in different cereals and cereal-derived food

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MYCOMASK
Abstract
An LC-MS/MS method was developed and validated for the simultaneous determination of deoxynivalenol, zearalenone, T-2-toxin, HT-2-toxin and metabolites, including 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol-3-glucoside, alpha-zearalenol, beta-zearalenol, zearalenone-4-glucoside, alpha-zearalenol-4-glucoside, beta-zearalenol-4-glucoside and zearalenone-4-sulfate in maize, wheat, oats, cornflakes and bread. Extraction was performed with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. After filtration, the extract was evaporated and the residue was redissolved in mobile phase for injection. The mobile phase, which consisted of a mixture of methanol and water with 10 mM ammonium acetate, was adjusted to pH 3 with glacial acetic acid. A sample clean-up procedure was not included because of the low recoveries of free and masked mycotoxins and their differences in polarity. The method allowed the simultaneous determination of 13 Fusarium mycotoxins in a one-step chromatographic run using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated for several parameters such as linearity, apparent recovery, limit of detection, limit of quantification, precision, expanded measurement uncertainty and specificity. The limits of detection varied from 5 to 13 ng g(-1); those for the limit of quantification from 10 to 26 ng g(-1). The results of the performance characteristics of the developed LC-MS/MS method were in good agreement with the criteria mentioned in Commission Regulation (EC) No. 401/2006. Thirty samples of a variety of food and feed matrices were sampled and analysed between July 2010 and January 2011.
Keywords
mycotoxins, zearalenone, mycotoxins, other, mycotoxins, trichothecenes, mycotoxins, Fusarium, survey, chromatography, LC/MS, animal feed, bread, cereals, cereals and grain, TANDEM MASS-SPECTROMETRY, ARABIDOPSIS-THALIANA, MYCOTOXINS, WHEAT, MAIZE, PHASE, HT-2, BEER

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Citation

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Chicago
De Boevre, Marthe, José Diana Di Mavungu, Peter Maene, Kris Audenaert, Dieter Deforce, Geert Haesaert, Mia Eeckhout, et al. 2012. “Development and Validation of an LC-MS/MS Method for the Simultaneous Determination of Deoxynivalenol, Zearalenone, T-2 Toxin and Some Masked Metabolites in Different Cereals and Cereal-derived Food.” Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment 29 (5): 819–835.
APA
De Boevre, M., Diana Di Mavungu, J., Maene, P., Audenaert, K., Deforce, D., Haesaert, G., Eeckhout, M., et al. (2012). Development and validation of an LC-MS/MS method for the simultaneous determination of deoxynivalenol, zearalenone, T-2 toxin and some masked metabolites in different cereals and cereal-derived food. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 29(5), 819–835.
Vancouver
1.
De Boevre M, Diana Di Mavungu J, Maene P, Audenaert K, Deforce D, Haesaert G, et al. Development and validation of an LC-MS/MS method for the simultaneous determination of deoxynivalenol, zearalenone, T-2 toxin and some masked metabolites in different cereals and cereal-derived food. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT. 2012;29(5):819–35.
MLA
De Boevre, Marthe, José Diana Di Mavungu, Peter Maene, et al. “Development and Validation of an LC-MS/MS Method for the Simultaneous Determination of Deoxynivalenol, Zearalenone, T-2 Toxin and Some Masked Metabolites in Different Cereals and Cereal-derived Food.” FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 29.5 (2012): 819–835. Print.
@article{2118803,
  abstract     = {An LC-MS/MS method was developed and validated for the simultaneous determination of deoxynivalenol, zearalenone, T-2-toxin, HT-2-toxin and metabolites, including 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol-3-glucoside, alpha-zearalenol, beta-zearalenol, zearalenone-4-glucoside, alpha-zearalenol-4-glucoside, beta-zearalenol-4-glucoside and zearalenone-4-sulfate in maize, wheat, oats, cornflakes and bread. Extraction was performed with acetonitrile/water/acetic acid (79/20/1, v/v/v) followed by a hexane defatting step. After filtration, the extract was evaporated and the residue was redissolved in mobile phase for injection. The mobile phase, which consisted of a mixture of methanol and water with 10 mM ammonium acetate, was adjusted to pH 3 with glacial acetic acid. A sample clean-up procedure was not included because of the low recoveries of free and masked mycotoxins and their differences in polarity. The method allowed the simultaneous determination of 13 Fusarium mycotoxins in a one-step chromatographic run using a Waters Acquity UPLC system coupled to a Quattro Premier XE mass spectrometer. The method was validated for several parameters such as linearity, apparent recovery, limit of detection, limit of quantification, precision, expanded measurement uncertainty and specificity. The limits of detection varied from 5 to 13 ng g(-1); those for the limit of quantification from 10 to 26 ng g(-1). The results of the performance characteristics of the developed LC-MS/MS method were in good agreement with the criteria mentioned in Commission Regulation (EC) No. 401/2006. Thirty samples of a variety of food and feed matrices were sampled and analysed between July 2010 and January 2011.},
  author       = {De Boevre, Marthe and Diana Di Mavungu, Jos{\'e} and Maene, Peter and Audenaert, Kris and Deforce, Dieter and Haesaert, Geert and Eeckhout, Mia and Callebaut, Alfons and Berthiller, Franz and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {1944-0049},
  journal      = {FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE \& RISK ASSESSMENT},
  keyword      = {mycotoxins,zearalenone,mycotoxins,other,mycotoxins,trichothecenes,mycotoxins,Fusarium,survey,chromatography,LC/MS,animal feed,bread,cereals,cereals and grain,TANDEM MASS-SPECTROMETRY,ARABIDOPSIS-THALIANA,MYCOTOXINS,WHEAT,MAIZE,PHASE,HT-2,BEER},
  language     = {eng},
  number       = {5},
  pages        = {819--835},
  title        = {Development and validation of an LC-MS/MS method for the simultaneous determination of deoxynivalenol, zearalenone, T-2 toxin and some masked metabolites in different cereals and cereal-derived food},
  url          = {http://dx.doi.org/10.1080/19440049.2012.656707},
  volume       = {29},
  year         = {2012},
}

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