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Anti body-induced internalization of viral glycoproteins and gE-gl Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis

Gerlinde Van de Walle UGent, Herman Favoreel UGent, Hans Nauwynck UGent and Maurice Pensaert (2003) JOURNAL OF GENERAL VIROLOGY. 84(4). p.939-948
abstract
Pseudorabies virus (PRV)-infected blood monocytes are able to transport virus throughout the body of vaccination-immune pigs. PRV-infected monocytes express viral glycoproteins in their plasma membrane that can be recognized by virus-specific antibodies. Recently, it has been shown that addition of PRV-specific polyclonal immunoglobulins to PRV-infected monocytes at 37degreesC induces internalization of the majority of plasma membrane-expressed viral glycoproteins. This study investigated whether this process may interfere with efficient antibody-dependent complement-mediated lysis (ADCML) of infected monocytes. Therefore, an ADCML assay was set up in vitro. A significant decrease in the percentage of cells lysed by ADCML was observed when antibody-induced internalization of PRV glycoproteins occurred (P<0.005). Furthermore, it is shown (i) that the PRV gE-gl complex, which, like certain other alphaherpesvirus orthologues, possesses IgG-binding capacity, aids in avoiding efficient ADCML of PRV-infected monocytes and (ii) that the efficiency of PRV gE-gl-mediated evasion of ADCML can be decreased by the presence of gE-gl-specific antibodies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
HERPES-SIMPLEX VIRUS-1, AUJESZKYS-DISEASE VIRUS, IMMUNOGLOBULIN-G, CELLS, PIGS, DESTRUCTION, ACTIVATION, ANTIBODIES, COMPONENT, SPREAD
journal title
JOURNAL OF GENERAL VIROLOGY
J. Gen. Virol.
volume
84
issue
4
pages
939-948 pages
Web of Science type
Article
Web of Science id
000182053500021
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.036 (2003)
JCR rank
24/132 (2003)
JCR quartile
1 (2003)
ISSN
0022-1317
DOI
10.1099/vir.0.18663-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
211723
handle
http://hdl.handle.net/1854/LU-211723
date created
2004-04-19 17:38:00
date last changed
2017-04-20 14:19:43
@article{211723,
  abstract     = {Pseudorabies virus (PRV)-infected blood monocytes are able to transport virus throughout the body of vaccination-immune pigs. PRV-infected monocytes express viral glycoproteins in their plasma membrane that can be recognized by virus-specific antibodies. Recently, it has been shown that addition of PRV-specific polyclonal immunoglobulins to PRV-infected monocytes at 37degreesC induces internalization of the majority of plasma membrane-expressed viral glycoproteins. This study investigated whether this process may interfere with efficient antibody-dependent complement-mediated lysis (ADCML) of infected monocytes. Therefore, an ADCML assay was set up in vitro. A significant decrease in the percentage of cells lysed by ADCML was observed when antibody-induced internalization of PRV glycoproteins occurred (P{\textlangle}0.005). Furthermore, it is shown (i) that the PRV gE-gl complex, which, like certain other alphaherpesvirus orthologues, possesses IgG-binding capacity, aids in avoiding efficient ADCML of PRV-infected monocytes and (ii) that the efficiency of PRV gE-gl-mediated evasion of ADCML can be decreased by the presence of gE-gl-specific antibodies.},
  author       = {Van de Walle, Gerlinde and Favoreel, Herman and Nauwynck, Hans and Pensaert, Maurice},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  keyword      = {HERPES-SIMPLEX VIRUS-1,AUJESZKYS-DISEASE VIRUS,IMMUNOGLOBULIN-G,CELLS,PIGS,DESTRUCTION,ACTIVATION,ANTIBODIES,COMPONENT,SPREAD},
  language     = {eng},
  number       = {4},
  pages        = {939--948},
  title        = {Anti body-induced internalization of viral glycoproteins and gE-gl Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis},
  url          = {http://dx.doi.org/10.1099/vir.0.18663-0},
  volume       = {84},
  year         = {2003},
}

Chicago
Van de Walle, Gerlinde, Herman Favoreel, Hans Nauwynck, and Maurice Pensaert. 2003. “Anti Body-induced Internalization of Viral Glycoproteins and gE-gl Fc Receptor Activity Protect Pseudorabies Virus-infected Monocytes from Efficient Complement-mediated Lysis.” Journal of General Virology 84 (4): 939–948.
APA
Van de Walle, Gerlinde, Favoreel, H., Nauwynck, H., & Pensaert, M. (2003). Anti body-induced internalization of viral glycoproteins and gE-gl Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis. JOURNAL OF GENERAL VIROLOGY, 84(4), 939–948.
Vancouver
1.
Van de Walle G, Favoreel H, Nauwynck H, Pensaert M. Anti body-induced internalization of viral glycoproteins and gE-gl Fc receptor activity protect pseudorabies virus-infected monocytes from efficient complement-mediated lysis. JOURNAL OF GENERAL VIROLOGY. 2003;84(4):939–48.
MLA
Van de Walle, Gerlinde, Herman Favoreel, Hans Nauwynck, et al. “Anti Body-induced Internalization of Viral Glycoproteins and gE-gl Fc Receptor Activity Protect Pseudorabies Virus-infected Monocytes from Efficient Complement-mediated Lysis.” JOURNAL OF GENERAL VIROLOGY 84.4 (2003): 939–948. Print.